Article
Cell & Tissue Engineering
Kyungtae Lim, Alex P. A. Donovan, Walfred Tang, Dawei Sun, Peng He, J. Patrick Pett, Sarah A. Teichmann, John C. Marioni, Kerstin B. Meyer, Andrea H. Brand, Emma L. Rawlins
Summary: Variation in lung alveolar development is linked to disease susceptibility. Researchers have identified an alveolar-fated epithelial progenitor in human fetal lungs and have grown them as self-organizing organoids to study cell lineage commitment. Using this system, they have discovered the role of Wnt signaling and the Wnt-NKX2.1 axis in alveolar differentiation, as well as the effects of genetic variation on this process.
Article
Medicine, Research & Experimental
Zhenbo Han, Zihang Xu, Ying Yu, Yang Cao, Zhengyi Bao, Xinlu Gao, Danyu Ye, Gege Yan, Rui Gong, Juan Xu, Lai Zhang, Wenya Ma, Xiuxiu Wang, Fan Yang, Hong Lei, Ye Tian, Shijun Hu, Djibril Bamba, Ying Li, Desheng Li, Changzhu Li, Ning Wang, Ying Zhang, Zhenwei Pan, Baofeng Yang, Benzhi Cai
Summary: This study reveals a previously unidentified role of m6A demethylase ALKBH5 in determining the cardiac lineage commitment of human embryonic stem cells, by regulating the components of H3K4 modifying enzyme complexes and enhancing the transcription of GATA4. Loss of function of ALKBH5 alters the expression of KDM5B and RBBP5 through impairing the stability of their mRNAs, which promotes the transcription of GATA4 by enhancing histone H3 Lys4 trimethylation at the promoter region.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Review
Cell Biology
Janet Rossant, Patrick P. L. Tam
Summary: There has been a renewed interest in studying human early embryonic development, and significant progress has been made through improved culture conditions and stem-cell-based models. However, the mechanisms and molecular pathways involved in lineage development and embryonic patterning are still not well understood.
DEVELOPMENTAL CELL
(2022)
Article
Cell & Tissue Engineering
Jorge O. Munera, Daniel O. Kechele, Carine Bouffi, Na Qu, Ran Jing, Pritiprasanna Maity, Jacob R. Enriquez, Lu Han, Ian Campbell, Maxime M. Mahe, Heather A. Mccauley, Xinghao Zhang, Nambirajan Sundaram, Jonathan R. Hudson, Adrian Zarsozo-Lacoste, Suman Pradhan, Kentaro Tominaga, J. Guillermo Sanchez, Alison A. Weiss, Praneet Chatuvedi, Jason R. Spence, Mariam Hachimi, Trista North, George Q. Daley, Christopher N. Mayhew, Yueh-Chiang Hu, Takanori Takebe, Michael A. Helmrath, James M. Wells
Summary: This study shows that pluripotent stem cell-derived human colonic organoids can develop a diverse population of immune cells, including functioning macrophages, which is crucial for modeling normal and disease processes.
Review
Cell & Tissue Engineering
Sonam Raj, Sushil K. Jaiswal, Melvin L. DePamphilis
Summary: This review article addresses the enigmatic role of the p53 transcription factor and tumor suppressor in programmed cell death during embryonic development. Contradictory results regarding the dependence of pluripotent embryonic stem cells on p53 for cell death are reconciled based on experimental conditions. The review concludes that p53 is not required for mouse embryonic development, but can accelerate programmed cell death in certain conditions. Additionally, DNA damage in differentiated cells induces p53-dependent cell cycle arrest and senescence.
Article
Cell & Tissue Engineering
Jingran Zhang, Guang Shi, Junjie Pang, Xing Zhu, Qingcai Feng, Jie Na, Wenbin Ma, Dan Liu, Songyang Zhou
Summary: Induced crotonylation in hESCs resulted in differentiation into the endodermal lineage. This induced modification led to transcriptomic changes and decreased glycolysis in hESCs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Cell Biology
Jingjing Li, Osmond Lao, Freya F. Bruveris, Liyuan Wang, Kajal Chaudry, Ziqi Yang, Nona Farbehi, Elizabeth S. Ng, Edouard G. Stanley, Richard P. Harvey, Andrew G. Elefanty, Robert E. Nordon
Summary: A microfluidic culture system was developed to simulate embryonic circulation and investigate the role of circulatory flow and shear stress in embryonic blood development. Human pluripotent stem cells were differentiated into embryonic hematopoietic cells, and the pathways of extra-embryonic and embryonic blood differentiation were delineated using single-cell transcriptomic analysis. The study found that circulatory flow and specific signaling molecules promote the formation of precursor cells that differentiate into different blood cell types.
Article
Environmental Sciences
Jingyan Li, Huimin Weng, Shuang Liu, Fan Li, Ke Xu, Shan Wen, Xi Chen, Chang Li, Yongmei Nie, Bin Liao, Jianming Wu, Fahsai Kantawong, Xiang Xie, Fengxu Yu, Guang Li
Summary: This study found the presence of micro- and nanoplastics in human blood and placentas, indicating embryonic exposure to these particles. Furthermore, the exposure to polystyrene nanoplastics (PS-NPs) was found to negatively impact the cardiac differentiation of human embryonic stem cells (hESCs), leading to immature cardiomyocytes and impaired contractility. Mechanistically, PS-NPs induced mitochondrial oxidative stress, activated P38/Erk MAPK signaling pathway, and blocked autophagy flux, reducing the pluripotency of hESCs. Consistent results were observed in zebrafish embryo models, with reduced cardiac contraction and blood flow after exposure to PS-NPs.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Review
Neurosciences
Carla Belmonte-Mateos, Cristina Pujades
Summary: The central nervous system exhibits a wide diversity of neurons with specific cell types and proportions in the appropriate locations. The coordination of proliferation and differentiation is crucial for generating brains with specific size and cell composition. Understanding how cell diversity arises from pluripotent progenitor cells and how progenitor potential changes over time is an important focus in developmental neurobiology.
FRONTIERS IN NEUROSCIENCE
(2022)
Review
Cell & Tissue Engineering
Kyle Lewis, Momoko Yoshimoto, Takanori Takebe
Summary: This review discusses the importance of the fetal liver niche in HSC expansion, which occurs during development and has great clinical potential. Emerging approaches to generate expandable HSC in cell culture are also discussed, including the use of cells or organoid models in combination with engineering and systems biology approaches. Delivery of HSC by understanding the molecular and biological interactions between HSCs and fetal liver cells for their controlled maturation and expansion will be beneficial.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Multidisciplinary Sciences
Laura Jardine, Simone Webb, Issac Goh, Mariana Quiroga Londono, Gary Reynolds, Michael Mather, Bayanne Olabi, Emily Stephenson, Rachel A. Botting, Dave Horsfall, Justin Engelbert, Daniel Maunder, Nicole Mende, Caitlin Murnane, Emma Dann, Jim McGrath, Hamish King, Iwo Kucinski, Rachel Queen, Christopher D. Carey, Caroline Shrubsole, Elizabeth Poyner, Meghan Acres, Claire Jones, Thomas Ness, Rowen Coulthard, Natalina Elliott, Sorcha O'Byrne, Myriam L. R. Haltalli, John E. Lawrence, Steven Lisgo, Petra Balogh, Kerstin B. Meyer, Elena Prigmore, Kirsty Ambridge, Mika Sarkin Jain, Mirjana Efremova, Keir Pickard, Thomas Creasey, Jaume Bacardit, Deborah Henderson, Jonathan Coxhead, Andrew Filby, Rafiqul Hussain, David Dixon, David McDonald, Dorin-Mirel Popescu, Monika S. Kowalczyk, Bo Li, Orr Ashenberg, Marcin Tabaka, Danielle Dionne, Timothy L. Tickle, Michal Slyper, Orit Rozenblatt-Rosen, Aviv Regev, Sam Behjati, Elisa Laurenti, Nicola K. Wilson, Anindita Roy, Berthold Goettgens, Irene Roberts, Sarah A. Teichmann, Muzlifah Haniffa
Summary: Research demonstrates that the full blood and immune cell repertoire in fetal bone marrow is established within a short 6-7 week period in early second trimester, promoting rapid and extensive diversification of myeloid cells, with distinct differences from fetal liver.
Article
Allergy
Rafael Gras-Pena, Nichole M. Danzl, Mohsen Khosravi-Maharlooei, Sean R. Campbell, Amanda E. Ruiz, Christopher A. Parks, William Meng Suen Savage, Markus A. Holzl, Debanjana Chatterjee, Megan Sykes
Summary: This study developed a novel differentiation protocol to generate human thymic epithelial progenitors from human embryonic stem cells (hES-TEPs) and demonstrated their thymopoietic function in vivo. By incorporating hES-TEPs into a supportive thymic structure, the researchers enhanced human thymocyte development and increased the reconstitution of peripheral CD4+ naive T cells.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Engineering, Biomedical
Kejie Chen, Yi Zheng, Xufeng Xue, Yue Liu, Agnes M. Resto Irizarry, Huaijing Tang, Jianping Fu
Summary: Human embryonic stem cells have the ability to self-organize and form patterned tissues, and they can be manipulated in vitro to generate embryonic-like structures. A 3D suspension culture system has been developed to efficiently produce postimplantation, pre-gastrulation embryonic-like tissues, with the size of the initial cell seeding density impacting the development trajectory and resulting tissue types.
Review
Immunology
Shicheng Sun, Kevin Wijanarko, Oniko Liani, Kathleen Strumila, Elizabeth S. Ng, Andrew G. Elefanty, Edouard G. Stanley
Summary: Lymphoid cells, including T cells, B cells, NKT cells, and ILCs, play important roles in both adaptive and innate immune systems. While it is believed that these cells derive from HSCs in adult bone marrow, their ontogeny during embryogenesis is complex and involves progenitor cells predating the emergence of HSCs. Recent advancements in single cell RNA-sequencing and pluripotent stem cell-based developmental models provide new insights into lymphoid development and offer potential for clinical translation.
IMMUNOLOGICAL REVIEWS
(2023)
Article
Cell & Tissue Engineering
Jianan Jiang, Jinhua Qin, Jisheng Li, Xiaosong Lin, Bowen Zhang, Zeng Fan, Lijuan He, Quan Zeng, Wen Yue, Min Zheng, Xuetao Pei, Yanhua Li
Summary: This study found that the small molecule Ricolinostat can promote the differentiation of megakaryocytic cells from HSPCs, increase the proliferation of megakaryocytic cells, and decrease the secretion of IL-8 and the expression of CXCR2, thereby improving the efficiency of megakaryocytic differentiation of HSPCs.
STEM CELL RESEARCH & THERAPY
(2022)
Letter
Oncology
Helene Jakobczyk, Yan Jiang, Lydie Debaize, Benoit Soubise, Stephane Avner, Aurelien A. Serandour, Jeremie Rouger-Gaudichon, Anne-Gaelle Rio, Jason S. Carroll, Hana Raslova, David Gilot, Ziling Liu, Jocelyne Demengeot, Gilles Salbert, Nathalie Douet-Guilbert, Laurent Corcos, Marie-Dominique Galibert, Virginie Gandemer, Marie-Berengere Troadec
Article
Oncology
Paul Chaintreuil, Lucie Laplane, Florian Esnault, Victoria Ghesquier, Coline Savy, Nathan Furstoss, Marie-Laure Arcangeli, Thomas Cluzeau, Guillaume Robert, Nathalie Droin, Eric Solary, Patrick Auberger, Arnaud Jacquel
Summary: Macrophages are innate immune cells that play crucial roles in various physiological and pathological processes. Caspases, previously known for their involvement in programmed cell death, are found to have non-apoptotic functions in macrophage differentiation and inflammation. Emricasan, a pan-caspase inhibitor, has shown potential in reprogramming monocyte-derived macrophages and could be an alternative therapy for diseases driven by these macrophages.
Article
Hematology
Graciela Rabadan Moraes, Florence Pasquier, Christophe Marzac, Eric Deconinck, Carlotta Caterina Damanti, Gwendoline Leroy, Mira El-Khoury, Wassim El Nemer, Jean-Jacques Kiladjian, Hana Raslova, Albert Najman, William Vainchenker, Caroline Marty, Christine Bellanne-Chantelot, Isabelle Plo
Summary: This study identified an EPOR variant in a large family with JAK2-positive MPN, indicating the importance of inherited-risk alleles affecting the JAK2/STAT pathway in MPN. The findings suggest potential implications for risk assessment and treatment of MPN.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Editorial Material
Oncology
Eric Solary, Patricia Blanc, Michael Boutros, Charis Girvalaki, Franco Locatelli, Rene H. Medema, Peter Nagy, Josep Tabernero
Summary: UNCAN.eu is a European initiative aimed at advancing cancer research through the creation of a research data hub, leading to new developments in cancer care.
Editorial Material
Hematology
Cecile K. Lopez, Thomas Mercher
Summary: In this study, the authors used human and murine models to identify genes that are crucial for the survival of ecotropic virus integration site-1 (EVI1)-driven acute myeloid leukemia (AML) cells. They found that ERG is a conserved direct transcriptional target of EVI1, which plays a critical role in promoting cell survival and blocking differentiation in AML cells.
Meeting Abstract
Hematology
Anthony Hunter, Hannah Newman, Eric Solary, Klaus Geissler, Laura Palomo, Lurdes Zamora, Francesc Sole, Valeria Santini, Timothy A. Graubert, Swapna Thota, Elizabeth A. Griffiths, Lisa Pleyer, Felicitas R. Thol, Rafael Bejar, Luis E. Aguirre, David A. Sallman, Andrew Kuykendall, Rami S. Komrokji, Tracy George, Eric Padron
Article
Oncology
Zakia Aid, Elie Robert, Cecile K. Lopez, Maxence Bourgoin, Fabien Boudia, Melchior Le Mene, Julie Riviere, Marie Baille, Salima Benbarche, Laurent Renou, Alexandre Fagnan, Cecile Thirant, Laetitia Federici, Laure Touchard, Yann Lecluse, Anton Jetten, Birgit Geoerger, Helene Lapillonne, Eric Solary, Muriel Gaudry, Soheil Meshinchi, Francoise Pflumio, Patrick Auberger, Camille Lobry, Arnaud Petit, Arnaud Jacquel, Thomas Mercher
Summary: Pediatric acute myeloid leukemia expressing the ETO2::GLIS2 fusion oncogene has a poor prognosis. The expression of ETO2::GLIS2 leads to activation of CASP3 and increased cell death. Inhibiting both BCL2 and MCL1 is necessary to prevent disease progression in vivo, suggesting a potential therapeutic strategy for this aggressive pediatric AML subgroup.
Article
Biochemistry & Molecular Biology
Stephanie Solier, Michele Mondini, Lydia Meziani, Arnaud Jacquel, Catherine Lacout, Tom Vanden Berghe, Yvon Jule, Jean-Claude Martinou, Gerard Pierron, Julie Riviere, Marc Deloger, Corinne Dupuy, Anny Slama-Schwok, Nathalie Droin, Peter Vandenabeele, Patrick Auberger, Eric Deutsch, Jamel El-Benna, Pham My-Chan Dang, Eric Solary
Summary: Circulating monocytes are recruited to damaged tissues to generate macrophages that modulate disease progression. Activation of caspase-3 and caspase-7, located near mitochondria, is involved in the generation of monocyte-derived macrophages stimulated by Colony-stimulating factor-1 (CSF-1). A non-conventional pathway that involves caspases and activates NOX2 is responsible for CSF1-driven monocyte differentiation, and targeting this pathway may have therapeutic implications for modulating macrophage polarization in damaged tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Vincent Jachiet, Laure Ricard, Pierre Hirsch, Florent Malard, Laurent Pascal, Odile Beyne-Rauzy, Pierre Peterlin, Alexandre Thibault Jacques Maria, Norbert Vey, Maud D'Aveni, Marie-Pierre Gourin, Sophie Dimicoli-Salazar, Anne Banos, Stefan Wickenhauser, Louis Terriou, Benoit De Renzis, Eric Durot, Shanti Natarajan-Ame, Anne Vekhoff, Laurent Voillat, Sophie Park, Julien Vinit, Celine Dieval, Azeddine Dellal, Vincent Grobost, Lise Willems, Julien Rossignol, Eric Solary, Olivier Kosmider, Nicolas Dulphy, Lin Pierre Zhao, Lionel Ades, Pierre Fenaux, Olivier Fain, Mohamad Mohty, Beatrice Gaugler, Arsene Mekinian
Summary: Background systemic inflammatory and autoimmune diseases (SIADs) occur in a significant proportion of myelodysplastic syndrome (MDS) patients. The recently identified VEXAS syndrome, associated with somatic mutations in UBA1, is characterized by severe inflammatory conditions and hematological abnormalities, including MDS. However, the mechanisms underlying the association between MDS and SIADs are largely unknown. This study aimed to evaluate myeloid immune cell subsets in MDS patients with and without SIAD and compare them to healthy controls.
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
Meeting Abstract
Medicine, General & Internal
Z. Jevtic, M. R. Pique-Borras, F. Otzen Bagger, J. Seguin, M. Filgueira Bezerra, A. Louwagie, S. Juge, I. Nellas, R. Ivanek, A. Tzankov, U. M. Moll, O. Cantillo, R. Schulz-Heddergott, A. Fagnan, T. Mercher, J. Schwaller
SWISS MEDICAL WEEKLY
(2022)
Article
Medicine, Research & Experimental
Brahim Arkoun, Elie Robert, Fabien Boudia, Stefania Mazzi, Virginie Dufour, Aurelie Siret, Yasmine Mammasse, Zakia Aid, Matthieu Vieira, Imanci Aygun, Marine Aglave, Marie Cambot, Rachel Petermann, Sylvie Souquere, Philippe Rameau, Cyril Catelain, Romain Diot, Gerard Tachdjian, Olivier Hermine, Nathalie Droin, Najet Debili, Isabelle Plo, Sebastien Malinge, Eric Soler, Hana Raslova, Thomas Mercher, William Vainchenker
Summary: This study models the clonal evolution of Down syndrome acute megakaryoblastic leukemia (DS-AMKL) and reveals the roles of different mutations in differentiation blockage. The findings demonstrate the significant impact of GATA1s, SMC3, MPL, and T21 mutations on megakaryocyte differentiation and leukemic progression.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Meeting Abstract
Pediatrics
S. Tauchmann, F. O. Bagger, T. Bock, R. Sivalingam, T. Eder, A. Fagnan, M. von Lindern, T. Mercher, F. Grebien, J. Schwaller
KLINISCHE PADIATRIE
(2022)
Article
Hematology
Lamia Lamrani, Frederic Adam, Christelle Soukaseum, Cecile Denis, Hana Raslova, Jean-Philippe Rosa, Marijke Bryckaert
Summary: This study evaluates the role of filamin A (FLNa) subdomains in regulating integrin alpha IIb beta 3 signaling. The results show that FLNa-actin and FLNa-beta 3 interactions negatively regulate alpha IIb beta 3 activation, while FLNa dimerization domain negatively regulates alpha IIb beta 3 outside-in signaling.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Multidisciplinary Sciences
Salima Benbarche, Cecile K. Lopez, Eralda Salataj, Zakia Aid, Cecile Thirant, Marie-Charlotte Laiguillon, Severine Lecourt, Yannis Belloucif, Camille Vaganay, Marion Antonini, Jiang Hu, Alexandra da Silva Babinet, Delphine Ndiaye-Lobry, Bryann Pardieu, Arnaud Petit, Alexandre Puissant, Julie Chaumeil, Thomas Mercher, Camille Lobry
Summary: This study reveals that fusion oncogenes like ETO2-GLIS2 can induce the activation of Super Enhancers (SEs) that regulate essential gene modules synergizing for leukemia progression, promoting the growth and survival of leukemia cells.
