Article
Biochemistry & Molecular Biology
Fahad S. M. Alshehri, Claire S. Whyte, Ahmet Tuncay, Maria-Louise Williams, Heather M. Wilson, Nicola J. Mutch
Summary: FXIII is a transglutaminase that promotes thrombus stability by cross-linking fibrin. Monocytes and THP-1 cells exhibit up-regulation of FXIII-A antigen and activity in response to IL-4 and IL-10 stimulation, leading to enhanced stability of thrombi against fibrinolytic degradation. Membrane-associated FXIII-A in monocytes appears to play a crucial role in thrombus stabilization, particularly through a transglutaminase-dependent mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Rahim Kanji, Ying X. Gue, Vassilios Memtsas, Diana A. Gorog
Summary: The formation and fibrinolysis resistance of thrombus are influenced by factors such as platelet content and environment. Thrombi with high platelet content demonstrate significant resistance to fibrinolysis and are stabilized by various factors. Further research is needed to fully understand the mechanisms behind fibrinolysis resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Vassilios P. Memtsas, Deepa R. J. Arachchillage, Diana A. Gorog
Summary: This review article discusses the pathophysiological role and laboratory assessment of fibrin, factor XIII, and endogenous fibrinolysis in the final phase of coagulation cascade and fibrinolysis, as well as their involvement in various disease states and assessment of cardiovascular risk with the most up-to-date evidence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cardiac & Cardiovascular Systems
Anuj Narwal, Claire S. Whyte, Nicola J. Mutch
Summary: Thrombi in arteries are characterized by high platelet content and tightly packed fibrin, while those in veins are rich in red blood cells and fibrin with less platelet contribution. However, recent advances in thromboectomy and high-resolution imaging have challenged these traditional views on thrombi composition in different vascular beds. The distribution of fibrinolytic proteins within thrombi is influenced by the cellular composition and vascular bed, which in turn affects the susceptibility of thrombi to proteolytic degradation. Our current understanding of thrombus composition and its impact on thrombolytic therapy and thrombectomy is still in its early stages.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Claire S. Whyte, Hadj Ahmed. Mostefai, Kim M. Baeten, Andrew J. Lucking, David E. Newby, Nuala A. Booth, Nicola J. Mutch
Summary: The resolution of arterial thrombi relies on the endogenous fibrinolytic system. tPA primarily localizes to the thrombus head in a free and active form. Thrombi formed under high shear conditions are more resistant to fibrinolysis due to less tPA and increased PAI-1 concentrations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Shiho Amano, Kohei Oka, Yutaka Sato, Chiaki Sano, Ryuichi Ohta
Summary: Factor XIII (FXIII) deficiency is a rare but serious coagulation disorder. The diagnosis and treatment, especially the immunological testing for acquired FXIII deficiency, still face challenges due to limited facilities.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Hematology
Joanne L. Mitchell, Gemma Little, Alexander P. Bye, Renato S. Gaspar, Amanda J. Unsworth, Neline Kriek, Tanya Sage, Alexander Stainer, Ibidayo Sangowawa, Gael B. Morrow, Ricardo N. Bastos, Susan Shapiro, Michael J. R. Desborough, Nicola Curry, Jonathan M. Gibbins, Claire S. Whyte, Nicola J. Mutch, Christopher I. Jones
Summary: This study identifies the role of platelet FXIII-A in platelet function. Platelet FXIII-A enhances fibrinogen binding to the platelet surface and improves platelet aggregation and thrombus formation. FXIII-A is also involved in platelet spreading and clot retraction.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Cardiac & Cardiovascular Systems
Marina Marchetti, Patricia Gomez-Rosas, Laura Russo, Sara Gamba, Eleonora Sanga, Cristina Verzeroli, Chiara Ambaglio, Francesca Schieppati, Francesco Restuccia, Ezio Bonanomi, Marco Rizzi, Stefano Fagiuoli, Andrea D'Alessio, Grigorios T. Gerotziafas, Luca Lorini, Anna Falanga
Summary: This study provides a comprehensive characterization of hemostatic alterations in hospitalized COVID-19 patients, highlighting the correlation with inflammatory status. Fibrinolytic proteins and NLR may serve as predictors of thrombotic and hemorrhagic complications, respectively.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Hematology
Laura Somodi, Ildiko Beke Debreceni, Greta Kis, Marco Cozzolino, Janos Kappelmayer, Miklos Antal, Gyorgy Panyi, Helga Bardos, Nicola J. Mutch, Laszlo Muszbek
Summary: Receptor-mediated activation can expose cFXIII on the surface of more than 60% of platelets, while non-receptor mediated activation failed to do so, indicating that the translocation of cFXIII requires additional receptor-mediated mechanisms.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Hematology
Akbar Dorgalaleh
Summary: Despite the early discovery of FXIII, the diagnosis of FXIII deficiency was delayed until 1960 due to the normality of routine coagulation testing. The clot solubility test, once used to detect FXIII deficiency, is no longer recommended. Over the past 60 years, extensive knowledge about FXIII deficiency has been gained, with 197 mutations reported between 1992 and 2022. Therapeutic options have evolved to plasma-derived and recombinant FXIII concentrates. This review covers various aspects of FXIII deficiency, including its discovery, associated disorders, molecular basis, diagnosis, and treatment.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2023)
Review
Biochemistry & Molecular Biology
Katalin Dull, Fruzsina Fazekas, Daniel Torocsik
Summary: Research indicates that FXIII-A is not only involved in blood clotting, but may also play a role in various diseases across a wide range of fields; studies explore the role of FXIII-A in wound healing, chronic inflammatory diseases, atherosclerosis, etc., using multiple approaches; the field has come to recognize that FXIII-A is not just a marker, but actively involved in inflammatory and malignant diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Akbar Dorgalaleh
Summary: The prevalence of heterozygous FXIII deficiency ranges from 1 per 1,000 to 5,000 individuals, with a higher incidence in southeastern Iran. A study identified 49 different variants in the F13A gene, mostly missense, which were frequently found in the catalytic domain of the FXIII-A protein. While heterozygous FXIII deficiency is generally asymptomatic, it can lead to hemorrhagic complications in situations that challenge hemostasis.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2023)
Article
Hematology
Tsukasa Osaki, Chikako Yokoyama, Yasuo Magari, Masayoshi Souri, Akitada Ichinose
Summary: This study generated rat monoclonal antibodies against human FXIII-B and used them to create an immunochromatographic test (ICT) to detect anti-FXIII-B autoantibodies and distinguish between AiF13D and acF13D. The ICT showed similar precision to dot-blot analysis. Combining this ICT with the previously developed ICT for anti-FXIII-A autoantibodies may reduce false negatives and enable accurate diagnosis and treatment.
THROMBOSIS AND HAEMOSTASIS
(2023)
Review
Biochemistry & Molecular Biology
Michal Zabczyk, Joanna Natorska, Anetta Undas
Summary: FXIII is crucial in the modulation of fibrin clot properties in patients with VTE, impacting clot structure and function. FXIII activity is significantly reduced in acute PE and DVT patients, but gradually increases after the acute phase. Reduced FXIII activity is associated with impaired clot permeability and hypofibrinolysis in acute PE patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Hiroshi Kobayashi, Sho Matsubara, Chiharu Yoshimoto, Hiroshi Shigetomi, Shogo Imanaka
Summary: This review summarizes the biological functions of TFPI1 and TFPI2 in preeclamptic patients and discusses future directions in preeclampsia research. TFPI1 is an important physiological inhibitor of the TF-initiated extrinsic coagulation pathway, while TFPI2 inhibits fibrinolysis and exerts antifibrinolytic activity. TFPI1 and TFPI2 may play important roles in regulating the coagulation and fibrinolytic system and trophoblast invasion. Concentrations of TF, TFPI1, and TFPI2 are significantly altered in preeclamptic women compared to normal pregnancies. The TFPI protein family may serve as predictive biomarkers for preeclampsia and guide precision therapy.
Article
Hematology
Laura Somodi, Ildiko Beke Debreceni, Greta Kis, Marco Cozzolino, Janos Kappelmayer, Miklos Antal, Gyorgy Panyi, Helga Bardos, Nicola J. Mutch, Laszlo Muszbek
Summary: Receptor-mediated activation can expose cFXIII on the surface of more than 60% of platelets, while non-receptor mediated activation failed to do so, indicating that the translocation of cFXIII requires additional receptor-mediated mechanisms.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Biochemistry & Molecular Biology
Inci Kurt-Celep, Ayse Nihan Kilinc, Martin Griffin, Dilek Telci
Summary: In wound healing, TG2 enzyme plays a dual role in regulating extracellular matrix stabilization and promoting cell migration. Nitric oxide (NO) is involved in the regulation of TG2 activity, affecting cell migration, MMP activation, and the interaction between TG2 and its binding partners. Different MMPs showed varied responses to TG2 inhibition by NO, and TGF-beta 1 played a role in this process.
Article
Biochemistry & Molecular Biology
Claire S. Whyte, Akriti Rastogi, Ellis Ferguson, Michela Donnarumma, Nicola J. Mutch
Summary: This study compared the efficacy of cryoprecipitate and two fibrinogen concentrates in restoring clot integrity in a trauma-induced coagulopathy (TIC) model. Results showed that FibCLOT(R) was superior in normalizing clot structure and stabilizing clots against mechanical and fibrinolytic degradation compared to the other treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Nicola J. Mutch, Sam Walters, Elizabeth E. Gardiner, Owen J. T. McCarty, Simon F. De Meyer, Verena Schroeder, Joost C. M. Meijers
Summary: Bleeding and thrombosis are major clinical problems. Basic research in this field is facing funding challenges. The International Society on Thrombosis and Haemostasis (ISTH) established a task force to identify research opportunities and priorities in this area.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Engineering, Industrial
Dan Wang, Zitong Sheng, Xueqing Wang, Mark A. Griffin, Yiting Zhang, Ziying Wang
Summary: This study examines the impact of team safety stressors on employees' safety citizenship behaviors (SCBs) and identifies two mediating mechanisms. The results show that team safety stressors negatively predict both proactive and prosocial safety behaviors. Felt safety responsibility and affective commitment mediate the relationship between team safety stressors and the two types of SCB.
Article
Engineering, Industrial
Junwei Zheng, Xueqin Gou, Mark A. Griffin, Yang Miang Goh, Nini Xia
Summary: This study examines how temporal leadership influences individual safety behaviors by applying social information processing theory. The results show that attentiveness mediates the relationship between temporal leadership and safety compliance and safety participation. The study also identifies the moderating roles of team abusive supervision and individual safety consciousness in this relationship.
Article
Hematology
Gael B. Morrow, Nicola J. Mutch
Summary: Plasminogen activator inhibitor 1 (PAI-1), a SERPIN inhibitor, is primarily known for its regulation of fibrinolysis. However, it is now known that this inhibitor functions and contributes to many (patho)physiological processes including inflammation, wound healing, cell adhesion, and tumor progression. This review discusses the past, present, and future roles of PAI-1, with a particular focus on the discovery of this inhibitor in the 1970s and subsequent characterization in health and disease.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2023)
Article
Ergonomics
Michael David Wilson, Luke Strickland, Timothy Ballard, Mark A. Griffin
Summary: This article discusses the core characteristics of next-generation BMMs, including customization, individual-level parameter tuning, and real-time prediction. The study identifies challenges in advancing BMMs and emphasizes the importance of adopting multi-model frameworks, exploring emerging methods, and improving scientific practices in fatigue research.
THEORETICAL ISSUES IN ERGONOMICS SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Laura Somodi, Emoke Horvath, Helga Bardos, Barbara Barath, David Petho, Eva Katona, Jozsef Balla, Nicola J. Mutch, Laszlo Muszbek
Summary: Macrophages express FXIII-A, which cross-links proteins and may stabilize atherosclerotic plaque. These macrophages can become foam cells by accumulating oxLDL. FXIII-A is retained during the transformation into foam cells and its content increases. The presence of FXIII-A in the plaque suggests its involvement in plaque formation and structurization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Mohammed M. Jalal, Claire S. Whyte, Fraser P. Coxon, Nicola J. Mutch
Summary: In this study, it was found that atorvastatin (ATV) impairs the prenylation of Rab27b and Rap1a, leading to weakened platelet function. ATV delays clot formation and attenuates clot firmness, inhibits platelet aggregation and clot retraction, and reduces the binding of fibrinogen and P-selectin on activated platelets. Additionally, ATV affects the structure and stability of platelets and clots.
Article
Medicine, General & Internal
Pratima Chowdary, Banwari Agarwal, Maria Rita Peralta, Sanjay Bhagani, Simon Lee, James Goldring, Marc Lipman, Emal Waqif, Mark Phillips, Helen Philippou, Jonathan H. Foley, Nicola J. Mutch, Robert A. S. Ariens, Kathleen A. Stringer, Federico Ricciardi, Marie Watissee, Derralynn Hughes, Amit Nathwani, Anne Riddell, David Patch, Jim Buckley, Mark De Neef, Rahul Dimber, Cecilia Diaz-Garcia, Honey Patel, Aarti Nandani, Upuli Dissanayake, Nick Chadwick, Ahmed A. A. M. M. Alkhatip, Peter Watkinson, Eamon Raith, Suveer Singh, Tony Wolff, Rajeev Jha, Simon E. Brill, Ameet Bakhai, Alison Evans, Farhat Gilani, Keith Gomez
Summary: Nebulized recombinant tissue plasminogen activator (rt-PA) has the potential to improve lung injury and oxygenation in COVID-19 patients. Preliminary studies show better outcomes in patients receiving non-invasive respiratory support (NIRS). Randomized clinical trials are needed to confirm the clinical effects.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Hematology
Megan Simpson, Anuj Narwal, Eric West, Jill Martin, Catherine N. Bagot, Andrew R. Page, Henry G. Watson, Claire S. Whyte, Nicola J. Mutch
Summary: This study found that VITT patients show evidence of overt disseminated intravascular coagulation and fibrinogenolysis, which is mediated by dysregulated plasmin generation. These findings are important for understanding the clinical features and mechanisms of VITT.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Correction
Oncology
Giulia Pinton, Zhuo Wang, Cecilia Balzano, Sara Missaglia, Daniela Tavian, Renzo Boldorini, Dean A. Fennell, Martin Griffin, Laura Moro
FRONTIERS IN ONCOLOGY
(2022)
Article
Psychology, Applied
Ying Wang, Patrick D. Dunlop, Sharon K. Parker, Mark A. Griffin, Hazel Gachunga
Summary: The study found that the honesty-humility factor moderates the relationship between agreeableness and interpersonal competency, with individuals higher in honesty-humility showing a stronger association between agreeableness and interpersonal competency. This interaction effect was consistent across managerial roles in different cultural backgrounds.
APPLIED PSYCHOLOGY-AN INTERNATIONAL REVIEW-PSYCHOLOGIE APPLIQUEE-REVUE INTERNATIONALE
(2022)