Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Biochemical Research Methods
Renna L. Nouwairi, Larissa L. Cunha, Rachelle Turiello, Orion Scott, Jeff Hickey, Scott Thomson, Stuart Knowles, Jeff D. Chapman, James P. Landers
Summary: This article describes a field-forward prototype instrument capable of ultra-fast thermal cycling for real-time PCR amplification of DNA and RNA. The instrument can complete 40 cycles of real-time PCR in under 10 minutes, an 84% reduction in time compared to standard assays.
Article
Ophthalmology
Satoko Nakano, Yasuhiro Tomaru, Toshiaki Kubota, Hiroshi Takase, Manabu Mochizuki, Norio Shimizu, Sunao Sugita
Summary: The Direct Strip PCR test offers rapid and accurate detection of infectious pathogens causing uveitis. It shows high concordance with qPCR results, even when used by beginners, indicating its reliability for clinical use. Additionally, it can be used for intraoperative diagnosis, improving the prognosis of infectious diseases.
Article
Biochemical Research Methods
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hyejeong Jeong, Ki-Hun Jeong
Summary: A multi-channel plasmonic real-time reverse-transcription polymerase chain reaction system is used to demonstrate high-throughput and multi-target detection of the SARS-CoV-2 virus with four simultaneous reactions in a single assay within 15 min.
Article
Parasitology
Gereon Schares, Majda Globokar Vrhovec, Mareen Tuschy, Maike Joeres, Andrea Baerwald, Bretislav Koudela, Jitender P. Dubey, Pavlo Maksimov, Franz J. Conraths
Summary: The 529-bp repeat of H. hammondi can be used to establish a quantitative real-time PCR assay, with excellent specificity and sensitivity. This assay is a valuable diagnostic tool for epidemiological and cell biological studies on H. hammondi and related parasites.
PARASITES & VECTORS
(2021)
Article
Agriculture, Multidisciplinary
Lin Yao, Hongmei Xin, Meng Qu, Yanhua Jiang, Yingying Guo, Fengling Li, Na Li, Zhijun Tan, Lianzhu Wang
Summary: The study developed a duplex real-time PCR method to specifically, sensitively, and accurately detect oilfish and escolar-derived components. The method showed high specificity without cross-reaction on other species, with a limit of detection for DNA of 0.0002 ng μL(-1) for R. pretiosus and 0.002 ng μL(-1) for L. flavobrunneum. Oilfish- and escolar-derived components were detected in 12 of the 70 commercial samples, consistent with DNA barcoding test results.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Chemistry, Analytical
Padideh Mohammadyousef, Miltiadis Paliouras, Mark A. Trifiro, Andrew G. Kirk
Summary: In response to the need for rapid infectious pathogen detection, researchers have been working on adapting PCR for POC applications. This paper introduces a compact plasmonic PCR thermocycler for fast DNA amplification using photothermal heating with gold nanorods and a VCSEL. An ultrasensitive real-time amplicon detection strategy is also reported, based on monitoring UV absorption of the PCR sample cycle-by-cycle. This system allows for high detection sensitivity and does not require specific probes or fluorophores.
Article
Chemistry, Multidisciplinary
Shaw-Hwa Parng, Li-An Wu, Chien-Chih Kuo, Ping-Jung Wu, Yu-Yin Tsai, Chih-Hung Lee
Summary: This study proposes a method to generate droplets in a cylindrical tube for emulsion polymerase chain reaction. The validity of a non-planar droplet chip is verified by analyzing the light intensity of liquids in real-time during droplet processing. The study also compares two digital droplet PCR methods for measuring the copy numbers of EGFR.
Article
Microbiology
Daya Marasini, Melissa J. Whaley, Laurel T. Jenkins, Fang Hu, Wenxin Jiang, Nadav Topaz, Alex Chen, Susanna Schmink, Jennifer Dolan Thomas, Brian H. Harcourt, Henju Marjuki, Xin Wang
Summary: We have developed direct real-time PCR assays for the detection of Haemophilus influenzae (Hi) and its six serotypes directly from cerebrospinal fluid specimens. These assays are highly sensitive and specific, do not require DNA extraction, and can detect three serotypes in a single reaction, improving testing efficiency.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Food Science & Technology
Qiuyue Zheng, Xinying Yin, Aifu Yang, Ning Yu, Ranran Xing, Ying Chen, Ruijie Deng, Jijuan Cao
Summary: In this study, a real-time quantitative polymerase chain reaction (qPCR) method was developed for rapid detection of quinoa, coix seed, wild rice, and chickpea in food to authenticate the presence of these components. The qPCR method specifically identified the four source components and had a detection limit of 0.96, 1.14, 1.04, and 0.97 pg/μL for quinoa, coix seed, wild rice, and chickpea, respectively. The method was applicable to different food matrices and could be used for authenticity verification in deeply processed food.
Review
Medicine, General & Internal
Edinson Dante Meregildo-Rodriguez, Martha Genara Asmat-Rubio, Gustavo Adolfo Vasquez-Tirado
Summary: Tuberculosis is a global public health emergency. Improved diagnostic methods are urgently needed to address this epidemic. Droplet digital PCR (ddPCR) shows comparable diagnostic performance to qPCR for pulmonary tuberculosis, and better performance for extrapulmonary tuberculosis.
FRONTIERS IN MEDICINE
(2023)
Article
Optics
Damian Presti, Julian Bergier, Lucas Ripoll, Cristina Borio, Gustavo A. Torchia, Marcos Bilen
Summary: Since the global pandemic of SARS-CoV-2, the need for a low-cost and faster diagnostic tool for evaluating symptomatic patients has emerged. Point-of-care testing and point-of-need testing systems have been developed to provide accurate and rapid diagnostics. In this study, a bio-photonic device using an iso-thermal system was developed for detecting SARS-CoV-2. The device showed comparable sensitivity to the commercially used reference method and was built with simple and low-cost components, making it a high-efficiency and affordable instrument.
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION
(2023)
Article
Infectious Diseases
Alexander Oberli, Lavinia Furrer, Lena Skoko, Norbert Muller, Bruno Gottstein, Pascal Bittel
Summary: The study validated a real-time PCR technique that can distinguish Echinococcus multilocularis, E. granulosus s.l., and Taenia spp. in biopsy or cytology material. Sanger sequencing of E. granulosus s.l. and Taenia spp. amplicons enables differentiation of various Echinococcus and Taenia species. The assay demonstrated high sensitivity and specificity in detecting and identifying larval cestodiases in human tissue.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Oncology
Sarah Adel Hakim, Hoda Hassan Abou Gabal
Summary: This study demonstrated that the combined use of the three nuclear markers BAP1, EZH2, and Survivin enhances diagnostic sensitivity in discriminating pleural EM from RMH. The highest diagnostic accuracy and sensitivity were achieved with the combination of all three markers, while the combination of Survivin and EZH2 showed comparable results.
PATHOLOGY & ONCOLOGY RESEARCH
(2021)
Article
Neurosciences
Elisabetta Belli, Chiara Milano, Ilaria Pesaresi, Ilaria Trivelli, Antonio Tavoni, Eugenio Ciancia, Greta Ali, Virna Zampa, Chiara Pizzanelli, Gabriele Siciliano, Giulia Ricci
Summary: Intravascular large B-cell lymphoma is a rare lymphoma that can involve multiple organs, with unspecific clinical presentation and challenging diagnosis. Tissue biopsy is crucial for definitive diagnosis.
INTERNATIONAL JOURNAL OF NEUROSCIENCE
(2023)
Article
Endocrinology & Metabolism
A. Basolo, A. M. Poma, R. Giannini, G. Ceccarini, C. Pelosini, P. Fierabracci, M. U. Castany, S. Bechi Genzano, C. E. Ambrosini, G. Materazzi, L. Chiovato, F. Basolo, F. Santini, L. Torregrossa
Summary: Obese patients may have increased serum TSH concentrations, potentially due to adipocyte and lymphocyte infiltration of the thyroid. Gene expression profiling showed differences in metabolic and immune pathways in thyroid tissues of obese patients compared to normal-weight individuals.
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
(2022)
Article
Endocrinology & Metabolism
A. M. Poma, D. Bonuccelli, R. Giannini, E. Macerola, P. Vignali, C. Ugolini, L. Torregrossa, A. Proietti, M. Pistello, A. Basolo, F. Santini, A. Toniolo, F. Basolo
Summary: The study found that the SARS-CoV-2 virus can spread to endocrine organs in severe cases of COVID-19, with evidence of virus presence in abdominal subcutaneous adipose tissue, testes, and thyroid samples; Further research is needed to assess the occurrence rate of this phenomenon in clinically mild cases; Potential long-term effects of COVID-19 on endocrine functions should be taken into consideration.
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
(2022)
Article
Oncology
Elena Vigliar, Pasquale Pisapia, Filippo Dello Iacovo, Eduardo Alcaraz-Mateos, Greta Ali, Syed Z. Ali, Zubair W. Baloch, Claudio Bellevicine, Massimo Bongiovanni, Pavlina Botsun, Dario Bruzzese, Lukas Bubendorf, Reinhard Buttner, Sule Canberk, Arrigo Capitanio, Chiara Casadio, Eugeniu Cazacu, Beatrix Cochand-Priollet, Alessandro D'Amuri, Katelynn Davis, Catarina Eloy, Marianne Engels, Guido Fadda, Gabriella Fontanini, Franco Fulciniti, Paul Hofman, Antonino Iaccarino, Antonio Ieni, Xiaoyin Sara Jiang, Kennichi Kakudo, Izidor Kern, Ivana Kholova, Kathryn M. Linton McDermott, Chinhua Liu, Anandi Lobo, Maria D. Lozano, Umberto Malapelle, Zahra Maleki, Pamela Michelow, Michael W. Mikula, Jamal Musayev, Gonca Ozgun, Meltem Oznur, Francisca Maria Peiro Marques, David Poller, Michal Pyzlak, Betsy Robinson, Esther Diana Rossi, Sinchita Roy-Chowdhuri, Mauro Saieg, Spasenija Savic Prince, Fernando C. Schmitt, Francisco Javier Segui Ivanez, Tajana Stoos-Veic, Oksana Sulaieva, Brenda J. Sweeney, Giovanni Tuccari, Marie-Louise Van Velthuysen, Paul A. VanderLaan, Philippe Vielh, Patrizia Viola, Quirinus J. M. Voorham, Birgit Weynand, Pio Zeppa, William C. Faquin, Martha Bishop Pitman, Giancarlo Troncone
Summary: This study investigated the impact of the COVID-19 pandemic on cytological practices worldwide. The results showed a persistent reduction in cytological specimen volume during the post-lockdown period, but a significant increase in malignancy rates, indicating a gradual return to normalcy.
CANCER CYTOPATHOLOGY
(2022)
Article
Endocrinology & Metabolism
A. Basolo, A. M. Poma, D. Bonuccelli, A. Proietti, E. Macerola, C. Ugolini, L. Torregrossa, R. Giannini, P. Vignali, F. Basolo, F. Santini, A. Toniolo
Summary: In fatal cases of COVID-19, a significant proportion of adipocytes have been found to contain the SARS-CoV-2 nucleocapsid antigen, indicating direct infection of subcutaneous fat cells by the virus. Infection seems to activate the IFN alpha pathway and attract infiltrating leukocytes. Due to the large numbers of adipocytes in adults, adipose tissue serves as a significant reservoir for SARS-CoV-2 and an important source of inflammatory mediators.
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION
(2022)
Review
Endocrinology & Metabolism
Francesca Orsolini, Alessandro Prete, Pierpaolo Falcetta, Domenico Canale, Fulvio Basolo, Greta Ali, Francesca Manassero, Paolo Vitti, Rossella Elisei, Eleonora Molinaro
Summary: This article reports the first known case of asymptomatic bilateral testicular metastases of medullary thyroid cancer (MTC). It highlights the importance of considering the testis as a potential metastatic site of MTC, especially in patients with advanced disease.
EUROPEAN THYROID JOURNAL
(2022)
Article
Clinical Neurology
Vincenzo Montano, Michelangelo Mancuso, Costanza Simoncini, Francesca Torri, Lucia Chico, Greta Ali, Anna Rocchi, Fulvia Baldinotti, Maria Adelaide Caligo, Giovanna Lattanzi, Elisabetta Mattioli, Giovanna Cenacchi, Andrea Barison, Gabriele Siciliano, Giulia Ricci
Summary: This article presents a case of a patient with limb-girdle weakness and a double trouble scenario - mitochondrial DNA single deletion and a new LMNA mutation. Pathophysiological aspects were investigated using various methods, and implications arising from the hypothetical coexistence of two different pathological conditions were discussed.
JOURNAL OF NEUROMUSCULAR DISEASES
(2022)
Article
Endocrinology & Metabolism
Alessio Basolo, Anello Marcello Poma, Elisabetta Macerola, Diana Bonuccelli, Agnese Proietti, Alessandra Salvetti, Paola Vignali, Liborio Torregrossa, Laura Evangelisti, Rebecca Sparavelli, Riccardo Giannini, Clara Ugolini, Fulvio Basolo, Ferruccio Santini, Antonio Toniolo
Summary: Infection by SARS-CoV-2 may lead to testicular dysfunction and affect male fertility. Testicular cells are commonly infected in lethal COVID-19 cases, which is associated with activation of interferon pathways and downregulation of testis-specific genes involved in spermatogenesis.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Multidisciplinary Sciences
Alberto D'Angelo, Huseyin Kilili, Robert Chapman, Daniele Generali, Ingeborg Tinhofer, Stefano Luminari, Benedetta Donati, Alessia Ciarrocchi, Riccardo Giannini, Roberto Moretto, Chiara Cremolini, Filippo Pietrantonio, Navid Sobhani, Debora Bonazza, Robert Prins, Seung Geun Song, Yoon Kyung Jeon, Giuseppina O. Pisignano, Mattia K. Cinelli, Stefan K. Bagby, Araxi O. K. Urrutia
Summary: The immune system plays a key role in cancer development. By using NanoString-based gene profiling, this study identifies transcriptional changes in immune cell-related genes that are associated with disease prognosis, providing valuable insights for clinical applications.
Review
Medicine, General & Internal
Maria Giovanna Mastromarino, Alessandra Lenzini, Vittorio Aprile, Greta Ali, Diana Bacchin, Stylianos Korasidis, Marcello Carlo Ambrogi, Marco Lucchi
Summary: The 2021 WHO classification of tumors of the pleura introduces significant changes in the codification of mesothelioma, going beyond the three current histological subtypes and including advancements in nuclear grading, architectural patterns, cytological features, and stromal features. The new classification also recognizes mesothelioma in situ as a diagnostic category and emphasizes the importance of immunohistochemistry and fluorescence in situ hybridization (FISH) in establishing the diagnosis of epithelioid mesothelioma. These changes have implications for prognosis and patient management, but also pose challenges in the differential diagnosis of pre-invasive tumors.
Article
Endocrinology & Metabolism
Alessio Basolo, Guido Salvetti, Domenico Giannese, Susanna Bechi Genzano, Giovanni Ceccarini, Riccardo Giannini, Gianluca Sotgia, Paola Fierabracci, Paolo Piaggi, Ferruccio Santini
Summary: This study compared various prediction formulas with measured creatinine clearance in obese patients and found that existing formulas had inaccuracies in this population. To improve accuracy, a new formula was developed and a BMI cut-off point was identified for its use in obese patients.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Endocrinology & Metabolism
Daniele Sgro, Alessandro Brancatella, Giuseppe Greco, Liborio Torregrossa, Paolo Piaggi, Nicola Viola, Teresa Rago, Fulvio Basolo, Riccardo Giannini, Gabriele Materazzi, Rossella Elisei, Ferruccio Santini, Francesco Latrofa
Summary: Prognosis is good for papillary thyroid carcinoma, noninvasive follicular thyroid neoplasia with papillary-like nuclear features, and follicular thyroid carcinoma, but poor for poorly differentiated thyroid carcinoma and anaplastic thyroid carcinoma. Ultrasound can be helpful in ruling out tall cell variant papillary thyroid carcinoma.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Article
Oncology
Agnese Proietti, Francesca Signorini, Riccardo Giannini, Anello Marcello Poma, Elisabetta Macerola, Liborio Torregrossa, Gabriele Materazzi, Alessio Basolo, Ferruccio Santini, Rossella Elisei, David Viola, Fulvio Basolo, Clara Ugolini
Summary: This study analyzed the clinicopathological characteristics of 989 patients with tall-cell variant of papillary thyroid carcinoma (TCPTC) and found that this type of cancer is not exclusively found in older patients. The study also revealed higher rates of local recurrence in the younger and older age groups, and a higher rate of distant recurrence in older patients. The results suggest that the prognosis of TCPTC is age-dependent and further studies are needed.
Article
Oncology
Duilio Divisi, Gabriella Di Leonardo, Massimiliano Venturino, Elisa Scarnecchia, Alessandro Gonfiotti, Domenico Viggiano, Marco Lucchi, Alessandro Bertani, Maria Giovanna Mastromarino, Roberto Crisci
Summary: This study analyzed the role of endobronchial ultrasound (EBUS) in the preoperative staging of non-small cell lung cancer (NSCLC) and found that it has high sensitivity, specificity, and accuracy. EBUS is a minimally invasive and easy procedure that ensures patient safety.
Article
Oncology
Irene Pecora, Clara Ugolini, Riccardo Giannini, Mirella Giordano, Caterina Vivaldi, Monica Lencioni, Stefano Santi, Valentina Massa, Giovanni Pallabazzer, Silvia Catanese, Francesca Salani, Mario Antonio Belluomini, Enrico Vasile, Chiara Cremolini, Gabriella Fontanini, Gianluca Masi, Lorenzo Fornaro
Summary: In this study, the impact of FLOT on the immune tumor microenvironment (TME) in gastric cancer (GC) was investigated. The results showed significant changes in immune gene expression after FLOT treatment, particularly in genes related to cytotoxicity, T-cell functions, complement system, tumor necrosis factor-superfamily, cell cycle, and regulation. Moreover, an association between changes in immune cell profiling and tumor regression was observed, suggesting a potential role of immune response in treatment outcomes.
