期刊
JOURNAL OF THEORETICAL BIOLOGY
卷 356, 期 -, 页码 201-212出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jtbi.2014.04.024
关键词
Signal propagation; Cellular tissues; Complex networks; Drug tests
资金
- Fundacao para a Ciencia e a Tecnologia, Portugal [SFRH/BPD/65427/2009, SFRH/BPD/81627/2011]
- FCT [DRI/DAAD/1208/2013]
- DAAD [DRI/DAAD/1208/2013]
- [PEst-OE/FIS/UI0618/2011]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/81627/2011] Funding Source: FCT
We introduce a simple procedure of multivariate signal analysis to uncover the functional connectivity among cells composing a living tissue and describe how to apply it for extracting insight on the effect of drugs in the tissue. The procedure is based on the covariance matrix of time resolved activity signals. By determining the time-lag that maximizes covariance, one derives the weight of the corresponding connection between cells. Introducing simple constraints, it is possible to conclude whether pairs of cells are functionally connected and in which direction. After testing the method against synthetic data we apply it to study intercellular propagation of Ca2+ waves in astrocytes following an external stimulus, with the aim of uncovering the functional cellular connectivity network. Our method proves to be particularly suited for this type of networking signal propagation where signals are pulse-like and have short time-delays, and is shown to be superior to standard methods, namely a multivariate Granger algorithm. Finally, based on the statistical analysis of the connection weight distribution, we propose simple measures for assessing the impact of drugs on the functional connectivity between cells. (C) 2014 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据