期刊
JOURNAL OF THE ROYAL SOCIETY INTERFACE
卷 9, 期 77, 页码 3229-3239出版社
ROYAL SOC
DOI: 10.1098/rsif.2012.0542
关键词
neutrophils; inflammation resolution; zebrafish; dynamic modelling; approximate Bayesian computation
资金
- Engineering and Physical Sciences Research Council (EPSRC), UK
- European Research Council
- MRC [G0701932]
- MRC Centre [G0700091]
- Wellcome Trust [GR077544AIA]
- EPSRC [EP/G042209/1, EP/H00453X/1] Funding Source: UKRI
- MRC [G0701932] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/H00453X/1, EP/G042209/1] Funding Source: researchfish
- Medical Research Council [G0700091B, G0701932, G0700091] Funding Source: researchfish
Following neutralization of infectious threats, neutrophils must be removed from inflammatory sites for normal tissue function to be restored. Recently, a new paradigm has emerged, in which viable neutrophils migrate away from inflammatory sites by a process best described as reverse migration. It has generally been assumed that this process is the mirror image of chemotaxis, where neutrophils are drawn into the areas of infection or tissue damage by gradients of chemotactic cues. Indeed, efforts are underway to identify cues that drive neutrophils away by the reverse process, fugetaxis. By using photoconvertible pigments expressed in neutrophils in transparent zebrafish larvae, we were able to image the position of each neutrophil during inflammation resolution in vivo. These neutrophil coordinates were analysed within a dynamic modelling framework, using different forms of the drift-diffusion equation with model selection and parameter estimation based on approximate Bayesian computation. This analysis revealed the experimental data were best fitted by a model incorporating a diffusion term but no drift term-where the presence of drift would indicate fugetaxis. This result, for the first time, provides rigorous data-driven evidence that reverse migration of neutrophils in vivo is not a form of fugetaxis, but rather a stochastic redistribution.
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