Article
Neurosciences
Sara Hall, Christina D. Orru, Geidy E. Serrano, Douglas Galasko, Andrew G. Hughson, Bradley R. Groveman, Charles H. Adler, Thomas G. Beach, Byron Caughey, Oskar Hansson
Summary: This study used α Syn RT-QuIC to analyze CSF samples from patients with Lewy body disorders (LBD), and found that it has high sensitivity and specificity for identifying LBD cases with clinicopathological confirmation. However, it has lower sensitivity for non-standard LBD or asymptomatic LBD cases.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2022)
Article
Neurosciences
Fabricio Ferreira de Oliveira, Marjorie Camara Miraldo, Eduardo Ferreira de Castro-Neto, Sandro Soares de Almeida, Sandro Luiz de Andrade Matas, Paulo Henrique Ferreira Bertolucci, Maria da Graca Naffah-Mazzacoratti
Summary: Behavioral features may predict proteinopathies in neurodegenerative diseases, study examined associations of CSF biomarkers with neuropsychiatric features in DLB compared with AD and controls. DLB patients showed more intense behavioral burden, biomarker ratios were similar to AD, and APOE epsilon 4 status influenced amyloidogenesis and tau pathology differently in DLB and AD.
JOURNAL OF ALZHEIMERS DISEASE
(2021)
Article
Immunology
Gary P. Wormser, Adriana Marques, Charles S. Pavia, Ira Schwartz, Henry M. Feder, Andrew R. Pachner
Summary: This article discusses the potential role of microorganisms in the development of Alzheimer's disease, focusing on whether Lyme disease is a cause of Alzheimer's disease or Lewy body dementia. The paper concludes that there is no convincing evidence supporting a causal relationship between Lyme disease and either Alzheimer's disease or Lewy body dementia.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Geriatrics & Gerontology
Hao Wang, Ling-Zhi Ma, Ze-Hu Sheng, Jia-Yao Liu, Wei-Yu Yuan, Fan Guo, Wei Zhang, Lan Tan
Summary: This study investigates the correlation between cerebrospinal fluid (CSF) clusterin and Alzheimer's disease biomarkers, as well as its potential role in protecting cognitive abilities in patients with mild cognitive impairment (MCI).
FRONTIERS IN AGING NEUROSCIENCE
(2023)
Article
Medicine, General & Internal
Anna Emdina, Peter Hermann, Daniela Varges, Sabine Nuhn, Stefan Goebel, Timothy Bunck, Fabian Maass, Matthias Schmitz, Franc Llorens, Niels Kruse, Paul Lingor, Brit Mollenhauer, Inga Zerr
Summary: The study suggests that CSF oc-synuclein may serve as a potential prognostic marker for disease progression, affective symptoms, and executive cognitive function in Parkinson's disease.
Article
Clinical Neurology
Kurt A. Jellinger
Summary: PDD and DLB are two major neurocognitive disorders in the spectrum of Lewy body diseases with overlapping clinical and neuropathological features. DLB has higher Braak LB scores and neuritic Braak stages, as well as more severe neuropathological features like Thal A beta phases and neuritic Braak stages. Worse prognosis in DLB is linked to increased Braak neuritic stages and more severe cerebral amyloid angiopathy.
PARKINSONISM & RELATED DISORDERS
(2022)
Review
Neurosciences
Tyler S. Saunders, Danni A. Gadd, Tara L. Spires-Jones, Declan King, Craig Ritchie, Graciela Muniz-Terrera
Summary: This systematic review evaluates the association between cerebrospinal fluid protein markers and cognition in neurodegenerative dementias. Some evidence suggests a correlation between certain markers and worse cognition in Alzheimer's disease, frontotemporal dementia, and typical cognitive ageing. However, due to the substantial heterogeneity between studies, no firm conclusions can be drawn. Future research should focus on improving standardization and reporting, as well as investigating the significance of novel markers.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2022)
Article
Medicine, General & Internal
Carmen Pena-Bautista, Lourdes Alvarez-Sanchez, Rosa Pascual, Maria Jose Moreno, Miguel Baquero, Consuelo Chafer-Pericas
Summary: This study evaluated the use of cerebrospinal fluid biomarkers in the differential diagnosis of Alzheimer's disease. The results showed that the ratio of A beta 42/A beta 40 had the highest discriminating capacity between Alzheimer's disease and non-Alzheimer's disease patients, and could be useful in clinical practice. Additionally, elevated levels of neurofilament light chain (NfL) were found in the frontotemporal dementia group, suggesting its potential for the differential diagnosis of this condition.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2023)
Review
Biochemistry & Molecular Biology
Eun Hae Kwon, Sabrina Tennagels, Ralf Gold, Klaus Gerwert, Leon Beyer, Lars Toenges
Summary: This paper emphasizes the importance of reliable objective markers for identifying individuals at risk of Parkinson's disease. Advances in biomarker discovery, particularly in the cerebrospinal fluid (CSF), have provided promising preliminary results. Potential biomarkers include alpha-synuclein species, markers of amyloid and tau pathology, neurofilament light chain, lysosomal enzymes, and markers of neuroinflammation. Metabolomics approaches have also offered insights into novel biological pathways. Genetic forms of PD can help identify subgroups suitable for targeted treatments. Further validation studies are needed to determine the most valuable CSF biomarkers or combinations for clinical and research purposes.
Article
Medical Laboratory Technology
Anders Abildgaard, Tina Parkner, Cindy Soendersoe Knudsen, Hanne Gottrup, Henriette Klit
Summary: This study found that tau/Aβ 42 ratios have the best diagnostic performance, but the estimated cut-off values for the ratios were slightly higher than previously reported. Therefore, adjustment of the cut-offs may be warranted when using CSF analysis to support a diagnosis of AD in a heterogeneous high-prevalence cohort.
CLINICA CHIMICA ACTA
(2023)
Review
Cell Biology
Augoustos Tsamourgelis, Peter Swann, Leonidas Chouliaras, John T. O'Brien
Summary: Dementia with Lewy Bodies (DLB) is the second most common neurodegenerative dementia. Proteomics has revealed protein dysregulation in the brain and peripheral tissues in DLB, which shares common features with other dementias but also has unique protein signatures. Identifying novel protein targets and diagnostic biomarkers could lead to new therapeutics and improved clinical trials for DLB.
AGEING RESEARCH REVIEWS
(2023)
Article
Neurosciences
Federico Paolini Paoletti, Lorenzo Gaetani, Giovanni Bellomo, Elena Chipi, Nicola Salvadori, Chiara Montanucci, Andrea Mancini, Marta Filidei, Pasquale Nigro, Simone Simoni, Nicola Tambasco, Massimiliano Di Filippo, Lucilla Parnetti
Summary: The pathophysiological processes and development of Parkinson's disease with mild cognitive impairment (PD-MCI) are still debated. This study investigated the CSF neurochemical profile and cognitive changes in PD-MCI, cognitively normal PD, prodromal Alzheimer's disease, and individuals with other neurological diseases. CSF biomarkers related to amyloidosis, tauopathy, neurodegeneration, synaptic damage, and glial activation were measured. The results showed that PD-MCI patients had higher levels of NfL/p-NfH ratio compared to PD-CN. Baseline levels of NfL, p-tau, and sTREM2 were associated with worsening cognitive impairment in PD-MCI after 2 years. Further investigations with larger cohorts and neuropathological verification are needed to understand the heterogeneity of PD-MCI.
NPJ PARKINSONS DISEASE
(2023)
Article
Clinical Neurology
Anna Dittrich, Nicholas J. J. Ashton, Henrik Zetterberg, Kaj Blennow, Anna Zettergren, Joel Simren, Tobias Skillback, Sara Shams, Alejandra Machado, Eric Westman, Michael Scholl, Ingmar Skoog, Silke Kern
Summary: This study investigates the relationship between chronic kidney disease (CKD) and neurodegenerative diseases. It shows that CKD patients have higher levels of plasma neurofilament light (P-NfL) compared to individuals with normal kidney function. Therefore, P-NfL may serve as a potential biomarker linking CKD to neurodegenerative diseases.
Article
Multidisciplinary Sciences
Eleanor Sinclair, Drupad K. Trivedi, Depanjan Sarkar, Caitlin Walton-Doyle, Joy Milne, Tilo Kunath, Anouk M. Rijs, Rob M. A. de Bie, Royston Goodacre, Monty Silverdale, Perdita Barran
Summary: This study used metabolomics profiling to identify changes in lipids in the sebum of PD patients, with alterations related to the carnitine shuttle, sphingolipid metabolism, arachidonic acid metabolism, and fatty acid biosynthesis. The findings suggest sebum can be a potential source for identifying biomarkers for PD.
NATURE COMMUNICATIONS
(2021)
Review
Clinical Neurology
Jon B. Toledo, Carla Abdelnour, Rimona S. Weil, Daniel Ferreira, Federico Rodriguez-Porcel, Andrea Pilotto, Kathryn A. Wyman-Chick, Michel J. Grothe, Joseph P. M. Kane, Angela Taylor, Arvid Rongve, Sonja Scholz, James B. Leverenz, Bradley F. Boeve, Dag Aarsland, Ian G. McKeith, Simon Lewis, Iracema Leroi, John P. Taylor
Summary: Dementia with Le bodies (DLB) is clinically characterized by visual hallucinations, fluctuations, rapid eye movement (REM) sleep behavioral disorder, and parkinsonism. However, neuropathological studies have shown the coexistence of Alzheimer's disease, TAR DNA-binding protein 43 (TDP-43), and cerebrovascular pathologies in DLB cases. These co-pathologies should be taken into consideration in clinical trials for DLB individuals.
ALZHEIMERS & DEMENTIA
(2023)
Article
Plant Sciences
James E. Hourston, Tina Steinbrecher, Jake O. Chandler, Marta Perez, Katrin Dietrich, Veronika Tureckova, Danuse Tarkowska, Miroslav Strnad, Fridtjof Weltmeier, Juliane Meinhard, Uwe Fischer, Karin Fiedler-Wiechers, Michael Ignatz, Gerhard Leubner-Metzger
Summary: The dynamic behavior of seeds in soil seed banks depends on their ability to act as environmental sensors and adjust their germination sensitivity thresholds. A study showed that prolonged incubation of sugar beet fruits at low temperature can induce secondary non-deep dormancy in apparently nondormant crop species. The mechanisms behind this cold-induced secondary dormancy include the accumulation of abscisic acid, reduction in embryo growth potential, and a block in weakening the endosperm.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Engineering, Biomedical
David Zeman, Kamila Revendova, Radovan Bunganic, Marketa Ryzi, Petra Masarovicova, Pavlina Kusnierova, Vera Kotrlova, Pavel Hradilek, David Stejskal, Vojtech Thon
Summary: This study compared the results of flow cytometry and conventional cytology for the analysis of cerebrospinal fluid cells. The results showed that pre-centrifuged CSF had higher cell yield but altered cell subpopulation percentages. Analysis of B-cell subpopulations required larger sample volumes or higher cell counts.
BIOMEDICAL PAPERS-OLOMOUC
(2023)
Article
Biochemistry & Molecular Biology
Kazumi Nakabayashi, Matthew Walker, Dianne Irwin, Jonathan Cohn, Stephanie M. Guida-English, Lucio Garcia, Iva Pavlovic, Ondrej Novak, Danuse Tarkowska, Miroslav Strnad, Marta Perez, Anne Seville, David Stock, Gerhard Leubner-Metzger
Summary: Molecular responses of plants to natural phytotoxins involve general and compound-specific mechanisms. This study reveals that the putative allelochemical MyA interferes with auxin homeostasis during seed germination, leading to the inhibition of seedling growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Jaroslav Nisler, Zuzana Pekna, Radka Koncitikova, Pavel Klimes, Alena Kadlecova, Nino Murvanidze, Stefaan P. O. Werbrouck, Lenka Plackova, David Kopecny, David Zalabak, Lukas Spichal, Miroslav Strnad
Summary: The study explores novel inhibitors of cytokinin degradation, which show increased selectivity and efficiency for agricultural and biotechnological applications. By investigating the structure-activity relationship of two series of CKX inhibitors, the researchers identified key substituents with enhanced selectivity for specific plant species and improved inhibitory activity. These inhibitors protect cytokinins from degradation and promote shoot regeneration in plants. Furthermore, the study also revealed clear differences in toxicity among the tested compounds.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Materials Science, Multidisciplinary
Barbora Parizkova, Ioanna Antoniadi, David J. Poxson, Michal Karady, Daniel T. Simon, Marek Zatloukal, Miroslav Strnad, Karel Dolezal, Ondrej Novak, Karin Ljung
Summary: State-of-the-art technology based on organic electronics can be used for flow-free delivery of organic substances with high spatial resolution. The organic electronic ion pump (OEIP) can transport cytokinins to intact plants, allowing for targeted drug micro applications and regulation of physiological processes in plants.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Review
Biochemistry & Molecular Biology
Dorota Konickova, Katerina Mensikova, Lucie Tuckova, Eva Henykova, Miroslav Strnad, David Friedecky, David Stejskal, Radoslav Matej, Petr Kanovsky
Summary: The understanding of neurodegenerative diseases has shifted in the past 20 years, from being seen as well-defined entities to complex and heterogeneous processes. Diagnosis primarily requires brain imaging techniques or invasive tests, but identifying specific proteinopathies is difficult due to overlap in clinical diagnoses. Laboratory methods to identify biomarkers are urgently needed to improve diagnosis, monitor disease progression, and evaluate treatment effects.
Review
Medicine, General & Internal
Jiri Plasek, Marie Lazarova, Jozef Dodulik, Patrik Sulc, David Stejskal, Zdenek Svagera, Frantisek Vsiansky, Jan Vaclavik
Summary: Secretoneurin is a neuropeptide with cardioprotective effects that regulates intracellular calcium handling by inhibiting CaMKII and modulates processes such as apoptosis, immune response, and cell cycle. It can also serve as a biomarker for predicting mortality in different disease states.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Jana Bibova, Veronika Kabrtova, Veronika Vecerova, Zuzana Kucerova, Martin Hudecek, Lenka Plackova, Ondrej Novak, Miroslav Strnad, Ondrej Plihal
Summary: Plasmodiophora brassicae is a pathogen that causes clubroot disease in cruciferous plants, leading to morphological changes and symptoms such as gall formation and leaf abscission. The homeostasis of cytokinin and auxin appears to be crucial for club development. Study on late-stage infected Arabidopsis thaliana showed changes in cytokinin levels and their role in disease progression.
Review
Medicine, General & Internal
Daniel Karas, Marek Buzga, David Stejskal, Petr Kocna, Pavol Holeczy, Adela Novotna, Zdenek Svagera
Summary: This review article focuses on the use of breath tests in the field of bariatrics and obesitology. It provides an introduction to breath test problematics, a brief history, and a description of how breath tests are used for monitoring processes and substances. Although the number of relevant studies is small, this review could promote the future use of breath testing in bariatric treatments.
Article
Plant Sciences
Jaroslav Nisler, Zuzana Kucerova, Radoslav Koprna, Roman Sobotka, Jana Slivkova, Stephen Rossall, Martina Spundova, Alexandra Husickova, Jan Pilny, Danuse Tarkowska, Ondrej Novak, Maria Skrabisova, Miroslav Strnad
Summary: Increasing crop productivity and mitigating yield losses under stressful conditions are major challenges in agriculture. A recently discovered compound, MTU, delays senescence and enhances the growth of wheat plants. Multiyear field trials demonstrate that treatment with MTU increases average grain yields of wheat and barley by 5-8%. This compound acts independently of cytokinins or other phytohormones and appears to be the only chemical known to affect the stability and activity of PSI.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Medicinal
Urte Sachleviciute, Gabriel Gonzalez, Marie Kvasnicova, Sarka Stepankova, Neringa Kleiziene, Aurimas Bieliauskas, Marek Zatloukal, Miroslav Strnad, Frank A. Slok, Miroslav Kvasnica, Algirdas Sackus, Asta Zukauskaite
Summary: A library of 3-aryl-3-azetidinyl acetic acid methyl ester derivatives was synthesized and evaluated for neuroprotective activity in salsolinol- and glutamate-induced neurodegeneration models. Compound 28 showed the highest neuroprotective effect, which may be driven by reductions in oxidative stress and caspase-3/7 activity.
ARCHIV DER PHARMAZIE
(2023)
Article
Biochemistry & Molecular Biology
Dorota Konickova, Katerina Mensikova, Katerina Klicova, Monika Chudackova, Michaela Kaiserova, Hana Prikrylova, Pavel Otruba, Martin Nevrly, Petr Hlustik, Eva Henykova, Michal Kaleta, David Friedecky, Radoslav Matej, Miroslav Strnad, Ondrej Novak, Lucie Plihalova, Raymond Rosales, Carlo Colosimo, Petr Kanovsky
Summary: Neurodegenerative diseases are a heterogeneous group of diseases that affect the nervous system. Clinical diagnosis of these diseases is challenging due to their variability and overlapping symptoms. This study aimed to identify biomarkers in the cerebrospinal fluid and serum that can aid in the differential diagnosis and differentiation of specific neurodegenerative diseases. The results suggest that the proposed biomarkers can be useful for distinguishing between certain types of neurodegenerative diseases.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Vaclav Mik, Tomas Pospisil, Federica Brunoni, Jiri Gruz, Vladimfra Nozkova, Claus Wasternack, Otto Miersch, Miroslav Strnad, Kristyna Flokova, Ondrej Novak, Jitka Siroka
Summary: This research presents a highly sensitive method for the reliable determination of seven OPDA-aa compounds in plants. The method was optimized for extraction, sample clean-up, and final analysis. Five endogenous (+)-OPDA-aa were identified and their levels were estimated in mechanically wounded Arabidopsis thaliana leaves, with a peak observed 60 minutes after the wounding. The synthetic and analytical methodologies developed in this study will contribute to the understanding of the biological significance of these metabolites in plants.
Article
Plant Sciences
Gabriel Gomez-Ocampo, Carlos D. Crocco, Jimena Cascales, Jana Oklestkova, Danuse Tarkowska, Miroslav Strnad, Santiago Mora-Garcia, Jose L. Pruneda-Paz, Miguel A. Blazquez, Javier F. Botto
Summary: B-Box-containing zinc finger transcription factor BBX21 inhibits hypocotyl elongation in Arabidopsis through the brassinosteroid pathway. It reduces sensitivity to synthetic active brassinosteroid 24-epiBL at low concentrations in simulated shade and represses the biosynthesis and signaling genes of brassinosteroids. BBX21 integrates brassinosteroid homeostasis and shade-light signaling to fine-tune hypocotyl elongation in Arabidopsis.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Vladimir Skalicky, Ioanna Antoniadi, Ales Pencik, Ivo Chamrad, Rene Lenobel, Martin F. Kubes, Marek Zatloukal, Asta Zukauskaite, Miroslav Strnad, Karin Ljung, Ondrej Novak
Summary: Auxin and cytokinin show subcellular distribution in Arabidopsis cells, forming hormone gradients. This finding provides important insights into the mechanisms of plant growth and development.
