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Biochemistry & Molecular Biology
Anita Lewczuk, Anna Boratynska-Jasinska, Barbara Zablocka
Summary: The aim of this study was to determine the best housekeeping gene for the I/R gerbil model in two parts of the hippocampus. The results showed that Hmbs was the most stable reference gene in the whole hippocampal formation, especially when used in combination with the highly expressed gene Gapdh, making it suitable for qRT-PCR normalization in all hippocampal regions in this model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
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Multidisciplinary Sciences
Kyu Ri Hahn, Hyun Jung Kwon, Yeo Sung Yoon, Dae Won Kim, In Koo Hwang
Summary: In this study, the researchers synthesized the Tat-CHIP fusion protein and successfully delivered it into brain cells. The results showed that Tat-CHIP has antioxidant and neuroprotective effects, making it a potential therapeutic agent against ischemic damage.
SCIENTIFIC REPORTS
(2022)
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Biochemistry & Molecular Biology
Xenia Puig-Bosch, Markus Ballmann, Stefan Bieletzki, Bernd Antkowiak, Uwe Rudolph, Hanns Ulrich Zeilhofer, Gerhard Rammes
Summary: Neurosteroids and benzodiazepines are GABA(A) receptor modulators with anxiolytic effects. This study aimed to investigate the effects of neurosteroids and their synthesis using a synthetic compound called XBD173, which promotes neurosteroidogenesis by binding to the translocator protein 18 kDa (TSPO). The results showed that XBD173 induced neurosteroidogenesis and the application of potentially synthesized neurosteroids did not impair hippocampal CA1-LTP, which is important for learning and memory. Therefore, TSPO ligands could be promising candidates for post-ischemic recovery with neuroprotection and without detrimental effects on synaptic plasticity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
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Biochemistry & Molecular Biology
Joon-Ha Park, Tae-Kyeong Lee, Dae-Won Kim, Hyejin Sim, Jae-Chul Lee, Jong-Dai Kim, Ji-Hyeon Ahn, Choong-Hyun Lee, Young-Myeong Kim, Moo-Ho Won, Soo-Young Choi
Summary: Salicin protects against ischemic brain injury by reducing oxidative stress and activating the PI3K/Akt/GSK3 beta pathway.
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Biochemistry & Molecular Biology
Maria Kawalec, Piotr Wojtyniak, Ewelina Bielska, Anita Lewczuk, Anna Boratynska-Jasinska, Malgorzata Beresewicz-Haller, Malgorzata Frontczak-Baniewicz, Magdalena Gewartowska, Barbara Zablocka
Summary: Transient ischemic attacks (TIA) occur due to temporary blockage of blood circulation in the brain. This study found that changes in mitochondrial dynamics, mitophagy, and biogenesis may contribute to a reduced susceptibility to TIA.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Malgorzata Beresewicz-Haller, Olga Krupska, Pawel Bochomulski, Danuta Dudzik, Anita Checinska, Wojciech Hilgier, Coral Barbas, Krzysztof Zablocki, Barbara Zablocka
Summary: The study found that mitochondrial GLS1 activity against ischemic episodes is regulated by PKC beta II. Through the modulation of GLS1 activity and remodeling of mitochondria after exposure to ischemia-reperfusion, the hippocampal CA2-4 and DG regions show strong resistance, while the susceptible CA1 region does not experience the same changes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Woosuk Kim, Hyun Jung Kwon, Hyo Young Jung, Kyu Ri Hahn, Yeo Sung Yoon, In Koo Hwang, Soo Young Choi, Dae Won Kim
Summary: Purpurin has significant therapeutic effects on oxidative stress in HT22 cells and ischemic damage in gerbil hippocampus by improving cell survival, reducing DNA fragmentation and reactive oxygen species, inhibiting apoptosis-related protein levels, and decreasing inflammatory responses.
MOLECULAR NEUROBIOLOGY
(2022)
Editorial Material
Cell Biology
Clement Bodineau, Mercedes Tome, Piedad del Socorro Murdoch, Raul V. Duran
Summary: The study reveals that glutamine metabolism regulates the AMPK-MTORC1 signaling pathway not only through glutaminolysis, but also through the ASNS-GABA shunt, demonstrating a crucial metabolic network.
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Clinical Neurology
Karthik Balakrishnan, Mohammad Hleihil, Musadiq A. Bhat, Robert P. Ganley, Markus Vaas, Jan Klohs, Hanns Ulrich Zeilhofer, Dietmar Benke
Summary: Cerebral ischemia, a common cause of neuronal death, lacks effective pharmacological treatment. However, researchers have developed a synthetic peptide (R1-Pep) that restores overly activated GABA(B) receptors, reducing neuronal excitability and death.
Article
Neurosciences
Mariana L. Casalia, Tina Li, Harrison Ramsay, Pablo J. Ross, Mercedes F. Paredes, Scott C. Baraban
Summary: Interneneurons play a significant role in neural circuit complexity and maintaining normal brain function. Research shows that there are similarities in transcription factor expression patterns between porcine and rodent embryos, suggesting that cortical and hippocampal interneurons could originate from a distinct MGE progenitor domain in pigs. Xenotransplantation experiments demonstrate that porcine MGE progenitors are able to migrate and differentiate into functional interneurons in rat hippocampus, indicating the potential of using porcine embryonic cells for interneuron-based therapies.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Cell Biology
Kyu Ri Hahn, Hyun Jung Kwon, Yeo Sung Yoon, Dae Won Kim, In Koo Hwang
Summary: In this study, a fusion protein PEP-1-PGK1 was synthesized that can cross the blood-brain barrier, and it was found that low concentrations of PEP-1-PGK1 can prevent neuronal stress and ischemia-related damage.
Article
Multidisciplinary Sciences
Juergen Graf, Chuanqiang Zhang, Stephan Lawrence Marguet, Tanja Herrmann, Tom Flossmann, Robin Hinsch, Vahid Rahmati, Madlen Guenther, Christiane Frahm, Anja Urbach, Ricardo Melo Neves, Otto W. Witte, Stefan J. Kiebel, Dirk Isbrandt, Christian A. Huebner, Knut Holthoff, Knut Kirmse
Summary: NKCC1 is the primary transporter for chloride uptake in immature principal neurons, and its function in vivo network dynamics and cognitive abilities remains unclear. The study found that deletion of NKCC1 from telencephalic glutamatergic neurons impacts neuronal synchrony in neonatal hippocampal brain slices, but has minor effects on network activity and behavioral performance in the long term.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Pharmacology & Pharmacy
Musadiq A. Bhat, Abolghasem Esmaeili, Elena Neumann, Karthik Balakrishnan, Dietmar Benke
Summary: GABA(B) receptors play a crucial role in controlling neuronal excitability, and their downregulation can lead to excitotoxicity. This study found that preventing the interaction between CHOP and GABA(B) receptors can restore their normal expression and reduce neuronal death.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Neurosciences
Mohammad Hleihil, Markus Vaas, Musadiq A. Bhat, Karthik Balakrishnan, Dietmar Benke
Summary: The activation of GABA(B) receptors with baclofen provides neuroprotection against excitotoxic death in cerebral ischemia. Sustained activation stabilizes receptor expression and reduces downregulation under excitotoxic conditions, preserving GABA(B) receptor-mediated inhibition. This restoration of GABA(B) receptors enhances neuronal survival and function, making them promising targets for neuroprotection in acute cerebral ischemia.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Article
Neurosciences
Anita Lewczuk, Barbara Zablocka, Malgorzata Beresewicz-Haller
Summary: The transcription factor Nrf2 plays a crucial role in regulating genes involved in antioxidant and anti-inflammatory processes, metabolic regulation, and other cellular functions. This study investigates the activation of Nrf2 in different regions of the hippocampus and its impact on endogenous neuroprotection against ischemia-reperfusion (I/R) injury. The results reveal that CA2-4,DG in the hippocampus exhibits higher Nrf2 activity compared to CA1, indicating a potential mechanism explaining the different sensitivities of these regions to I/R injury.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Nutrition & Dietetics
Woosuk Kim, Hyo Young Jung, Dae Young Yoo, Hyun Jung Kwon, Kyu Ri Hahn, Dae Won Kim, Yeo Sung Yoon, Soo Young Choi, In Koo Hwang
Summary: The study demonstrates that GPE-R has neuroprotective effects against ischemic damage and inflammation in the hippocampus, reducing locomotor hyperactivity and increasing neuronal survival. It also shows a decrease in pro-inflammatory cytokines and activated microglia after ischemia/reperfusion, indicating GPE-R as a potential agent for protecting neurons from ischemic damage.
Article
Cell Biology
Hyo Young Jung, Hyun Jung Kwon, Woosuk Kim, In Koo Hwang, Goang-Min Choi, In Bok Chang, Dae Won Kim, Seung Myung Moon
Summary: Tat-SH3GL2 protects neurons from oxidative and ischemic damage by reducing lipid peroxidation and inflammation, and improving synaptic plasticity after ischemia.
Review
Nutrition & Dietetics
Seung J. Baek, Bruce D. Hammock, In-Koo Hwang, Qingxiao Li, Naima Moustaid-Moussa, Yeonhwa Park, Stephen Safe, Nanjoo Suh, Sun-Shin Yi, Darryl C. Zeldin, Qixin Zhong, Jennifer Alyce Bradbury, Matthew L. Edin, Joan P. Graves, Hyo-Young Jung, Young-Hyun Jung, Mi-Bo Kim, Woosuk Kim, Jaehak Lee, Hong Li, Jong-Seok Moon, Ik-Dong Yoo, Yiren Yue, Ji-Young Lee, Ho-Jae Han
Summary: In the 21st century, the incidence of metabolic and chronic diseases, such as cancer, obesity, and inflammation-related diseases, has sharply increased, with inflammation and oxidative stress being major underlying causes. Natural products and their bioactive components are increasingly recognized as promising therapeutic agents for these diseases due to their antioxidant and anti-inflammatory properties. Research in this area has expanded significantly to include chemical identification, mechanism of action determination, food fortification, supplement development, and enhancing bioavailability and bioactivity through nanotechnology.
Article
Plant Sciences
Woosuk Kim, Hyun Jung Kwon, Hyo Young Jung, Soon-Sung Lim, Beom-Goo Kang, Yong-Bok Jo, Dong-Sool Yu, Soo Young Choi, In Koo Hwang, Dae Won Kim
Summary: The study showed that Cissus verticillata leaf extract has significant benefits in alleviating obesity and memory deficits induced by a high-fat diet in mice, with the improvement associated with increased levels of BDNF in the hippocampus.
Article
Plant Sciences
Woosuk Kim, Hyun Jung Kwon, Hyo Young Jung, Soon-Sung Lim, Beom-Goo Kang, Yong-Bok Jo, Dong-Sool Yu, Soo Young Choi, In Koo Hwang, Dae Won Kim
Summary: The Cissus verticillata leaf extracts (CVE) exhibited dose-dependent toxicity in HT22 cells, but could ameliorate oxidative and ischemic neuronal damage at suitable concentrations. In animal studies, administration of CVE decreased ischemia-induced motor activity, neuronal death, and inflammatory responses, indicating its potential as a functional food to prevent neuronal damage induced by ischemia.
Article
Neurosciences
Woosuk Kim, Hyun Jung Kwon, Hyo Young Jung, Kyu Ri Hahn, Yeo Sung Yoon, In Koo Hwang, Soo Young Choi, Dae Won Kim
Summary: Purpurin has significant therapeutic effects on oxidative stress in HT22 cells and ischemic damage in gerbil hippocampus by improving cell survival, reducing DNA fragmentation and reactive oxygen species, inhibiting apoptosis-related protein levels, and decreasing inflammatory responses.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kyu Ri Hahn, Woosuk Kim, Hyo Young Jung, Hyun Jung Kwon, Dae Won Kim, In Koo Hwang, Yeo Sung Yoon
Summary: Feeding mice a diet enriched with cuprizone can induce demyelination in the corpus callosum and increase neural progenitor cells in the dentate gyrus. This damage is more pronounced in young adult mice and may be associated with changes in BDNF and pCREB levels.
NEUROCHEMICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Hyo Young Jung, Hyun Jung Kwon, Woosuk Kim, Kyu Ri Hahn, Seung Myung Moon, Yeo Sung Yoon, Dae Won Kim, In Koo Hwang
Summary: Phosphoglycerate mutase 5 (PGAM5), a glycolytic enzyme, has been found to play a significant role in oxidative stress and ischemic damage. In this study, the authors investigated the effects of PGAM5 on HT22 hippocampal cells and gerbil hippocampus, demonstrating its neuroprotective effects and its ability to alleviate oxidative stress and ischemia-induced neuronal death.
NEUROCHEMISTRY INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Kyu Ri Hahn, Hyun Jung Kwon, Yeo Sung Yoon, Dae Won Kim, In Koo Hwang
Summary: In this study, the researchers synthesized the Tat-CHIP fusion protein and successfully delivered it into brain cells. The results showed that Tat-CHIP has antioxidant and neuroprotective effects, making it a potential therapeutic agent against ischemic damage.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Kyu Ri Hahn, Hyun Jung Kwon, Woosuk Kim, Hyo Young Jung, In Koo Hwang, Dae Won Kim, Yeo Sung Yoon
Summary: This study investigated the neuroprotective effects of SOD1 against cuprizone-induced demyelination and adult hippocampal neurogenesis in C57BL/6 mice. It was found that the fusion protein PEP-1-SOD1 could alleviate the damage caused by cuprizone in terms of demyelination and neurogenesis in the hippocampus.
NEUROCHEMICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Hyun Jung Kwon, Kyu Ri Hahn, Sung Min Nam, Yeo Sung Yoon, Seung Myung Moon, In Koo Hwang, Dae Won Kim
Summary: Purpurin has potent anti-oxidant and anti-inflammatory effects in brain damage. This study shows that purpurin can ameliorate the aging phenotypes induced by D-galactose and improve memory impairment in mice.
NEUROCHEMISTRY INTERNATIONAL
(2023)
Article
Veterinary Sciences
Dae Young Yoo, Woosuk Kim, Hyo Young Jung, In Koo Hwang
Summary: The expression of somatostatin (SST) in the hippocampus of rats increases during postnatal development but decreases after P21. This study suggests that SST is closely associated with the differentiation of neuroblasts in the hippocampus during postnatal development.
VETERINARY SCIENCES
(2023)
Article
Cell Biology
Kyu Ri Hahn, Hyun Jung Kwon, Yeo Sung Yoon, Dae Won Kim, In Koo Hwang
Summary: In this study, a fusion protein PEP-1-PGK1 was synthesized that can cross the blood-brain barrier, and it was found that low concentrations of PEP-1-PGK1 can prevent neuronal stress and ischemia-related damage.
Article
Medicine, Research & Experimental
Hyo Young Jung, Woosuk Kim, Kyu Ri Hahn, Min Soo Kang, Hyun Jung Kwon, Jung Hoon Choi, Yeo Sung Yoon, Dae Won Kim, Dae Young Yoo, Moo-Ho Won, In Koo Hwang
Summary: The study investigated the time-dependent changes in the expression of vacuolar H+-ATPase V1B2 in the hippocampus after transient forebrain ischemia, revealing a transient increase in ATP6V1B2 expression following ischemia. This increase may be a compensatory mechanism for ischemia-related dysfunction of ATP6V1B2 in the hippocampus.
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2021)
Article
Cell Biology
Dae Young Yoo, Hyo Young Jung, Woosuk Kim, Kyu Ri Hahn, Hyun Jung Kwon, Sung Min Nam, Jin Young Chung, Yeo Sung Yoon, Dae Won Kim, In Koo Hwang
Summary: Entacapone treatment promoted hippocampal neurogenesis and improved memory function through activating the BDNF-TrkB-pCREB pathway, while also antagonizing the effects of TrkB receptor antagonist.
NEURAL REGENERATION RESEARCH
(2021)
