期刊
POSTEPY W KARDIOLOGII INTERWENCYJNEJ
卷 11, 期 2, 页码 100-107出版社
TERMEDIA PUBLISHING HOUSE LTD
DOI: 10.5114/pwki.2015.52282
关键词
myocardial regeneration; Wharton's jelly mesenchymal stem cells; human umbilical cord matrix; acute myocardial infarction; first-in-man; safety; feasibility
资金
- 'Gift of Hope' Regenerative Medicine Foundation in Krakow
- 'For the Heart' Foundation in Krakow
- John Paul II Hospital in Krakow, Poland
- Jagiellonian University Medical College [K/ZDS/005644]
- NCBR STRATEGMED, National Centre for Research and Development, Poland [265761]
Introduction: In large-animal acute myocardial infarction (AMI) models, Wharton's jelly (umbilical cord matrix) mesenchymal stem cells (WJMSCs) effectively promote angiogenesis and drive functional myocardial regeneration. Human data are lacking. Aim: To evaluate the feasibility and safety of a novel myocardial regeneration strategy using human WJMSCs as a unique, allogenic but immuno-privileged, off-the-shelf cellular therapeutic agent. Material and methods: The inclusion criterion was first, large (LVEF <= 45%, CK-MB > 100 U/l) AMI with successful infarct-related artery primary percutaneous coronary intervention reperfusion (TIMI >= 2). Ten consecutive patients (age 32-65 years, peak hs-troponin T 17.3 +/- 9.1 ng/ml and peak CK-MB 533 +/- 89 U/l, sustained echo LVEF reduction to 37.6 +/- 2.6%, cMRI LVEF 40.3 +/- 2.7% and infarct size 20.1 +/- 2.8%) were enrolled. Results: 30 x 10(6) WJMSCs were administered (LAD/Cx/RCA in 6/3/1) per protocol at approximate to 5-7 days using a cell delivery-dedicated, coronary-non-occlusive method. No clinical symptoms or ECG signs of myocardial ischemia occurred. There was no epicardial flow or myocardial perfusion impairment (TIMI-3 in all; cTFC 45 +/- 8 vs. 44 +/- 9, p = 0.51), and no patient showed hs-troponin T elevation (0.92 +/- 0.29 <= 24 h before vs. 0.89 +/- 0.28 <= 24 h after; decrease, p = 0.04). One subject experienced, 2 days after cell transfer, a transient temperature rise (38.9 degrees C); this was reactive to paracetamol with no sequel. No other adverse events and no significant arrhythmias (ECG Hotter) occurred. Up to 12 months there was one new, non-index territory lethal AMI but no adverse events that might be attributable to WJMSC treatment. Conclusions: This study demonstrated the feasibility and procedural safety of WJMSC use as off-the-shelf cellular therapy in human AMI and suggested further clinical safety of WJMSC cardiac transfer, providing a basis for randomized placebo-controlled endpoint-powered evaluation.
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