Preventive effect of Polysaccharide of Larimichthys Crocea Swimming Bladder on Activated Carbon-Induced Constipation in Mice

Effects of polysaccharide of Larimichthys crocea swimming bladder (PLCSB) on activated carbon-induced constipation in ICR mice were investigated. ICR mice were subjected to oral administration with lactic acid bacteria for 9 days. Body weight, diet and drinking intake, defecation status, gastrointestinal transit, and defecation time, as well as motilin (MTL), gastrin (Gas), endothelin (ET), somatostatin (SS), acetylcholinesterase (AChE), substance P (SP), and vasoactive intestinal peptide (VIP) levels in serum were used to evaluate the preventive effects of PLCSB on constipation. Bisacodyl, a laxative drug, was used as a positive control. The time to the first black stool defecation for normal, control, bisacodyl-treated (100 mg/kg), 50 and 100 mg/kg PLCSB-treated mice were 88, 202, 126, 155, and 135 min, respectively. Following the consumption of oral administration of 50 and 100 mg/kg PLCSB or bisacodyl (100 mg/kg), the gastrointestinal transit was reduced to 62.6, 78.3, and 90.2%, respectively. The serum levels of mm, Gas, ET, AChE, SP, and VIP were significantly increased, and the serum levels of SS were reduced in the mice treated with PLCSB compared with those in the untreated control mice (p <0.05). These results demonstrate that PLCSB has preventive effects on mouse constipation and high concentration of that demonstrated the better functional activity.