期刊
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
卷 23, 期 8, 页码 1441-1449出版社
SOC BRASILEIRA QUIMICA
DOI: 10.1590/S0103-50532012005000001
关键词
retinol; alpha-tocopherol; lycopene; beta-carotene; HPLC; liquid-liquid extraction
资金
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior [CAPES/DAAD PROBRAL 352/10]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq/MCT) [479613/2009-5]
- CAPES/REUNI
- CNPq
A method for the simultaneous quantification of lycopene, beta-carotene, retinol and alpha-tocopherol by high-performance liquid chromatography (HPLC) with Vis/fluorescence detection with isocratic elution was optimized and validated. The method consists of a rapid and simple liquid-liquid extraction procedure and a posterior quantification of extracted supernatants by HPLC. Aliquots of plasma were stored at -20 degrees C for three months for stability study. The methodology was applied to samples from painters and individuals not exposed to paints (n = 75). The assay was linear for all vitamins (r > 0.99). Intra-and inter-run precisions were obtained with coefficient of variation smaller than 5%. The accuracies ranged from 0.29 to -5.80% and recoveries between 92.73 and 101.97%. Plasma samples and extracted supernatants were stable for 60 days at -20 degrees C. A significant decrease of lycopene, beta-carotene and retinol concentrations in plasma from exposed individuals compared to non-exposed individuals (p < 0.05) was observed. The method is simple, reproducible, precise, accurate and sensitive, and can be routinely utilized in clinical laboratories.
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