Article
Cell Biology
Anastasia Sudarikova, Mikhail Fomin, Regina F. Sultanova, Ying Zhao, Samantha Perez, Mark Domondon, Margarita Shamatova, Daria Lysikova, Denisha R. Spires, Daria Ilatovskaya
Summary: This study investigates the role of histamine-related pathways in the kidney, specifically in the collecting duct cells. The findings demonstrate the expression of histamine receptors and enzymes responsible for histamine metabolism and synthesis in these cells, as well as the effects of histamine on intracellular calcium and cAMP levels. Additionally, histamine inhibits the activity of epithelial sodium channels and decreases the expression of alpha ENaC. These results suggest potential implications of histamine in inflammation-related renal conditions.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Review
Urology & Nephrology
Minolfa C. Prieto, Alexis A. Gonzalez, Bruna Visniauskas, L. Gabriel Navar
Summary: The intrarenal renin-angiotensin system is crucial for regulating kidney function and blood pressure, especially the control in the collecting duct plays a key role in the development of hypertension and kidney injury.
NATURE REVIEWS NEPHROLOGY
(2021)
Article
Physiology
Hsiu-Hui Yang, Shih-Han Su, Cheng-Hsuan Ho, Ai-Hsin Yeh, Yi-Jiun Lin, Ming-Jiun Yu
Summary: The expression of the water channel protein AQP2 is regulated by the glucocorticoid receptor and the pituitary hormone vasopressin. Transcriptomic comparison revealed positive and negative regulatory pathways maintained by the glucocorticoid receptor for the vasopressin-induced Aqp2 gene expression.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Arnab Datta, Chin-Rang Yang, Karim Salhadar, Euijung Park, Chung-Lin Chou, Viswanathan Raghuram, Mark A. Knepper
Summary: This study systematically analyzed vasopressin-induced phosphorylation changes and identified at least six downstream protein kinases that are regulated by vasopressin, providing important insights for future research.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Eric R. Barros Lamus, Valentina Carotti, Christine R. S. de Vries, Femke Witsel, Onno J. Arntz, Fons A. J. van de Loo, Cristian A. Carvajal, Rene J. M. Bindels, Joost G. J. Hoenderop, Juan P. Rigalli
Summary: This study investigates the regulatory effect of EVs from renal proximal tubule and collecting duct cells on extracellular ATP levels and the expression of the alpha subunit of the epithelial sodium channel. The findings reveal that EVs can modulate purinergic signaling through mechanisms such as miRNA regulation and ATPase activity, impacting renal physiological and pathophysiological processes. Further research on EV release and cargo content may shed light on the role of EVs in renal purinergic signaling.
Article
Multidisciplinary Sciences
Xiaoyuan Qian, Junlai Wan, Yuanzhong Tan, Zhenrui Liu, Ying Zhang
Summary: This study investigated the prognostic factors and the impact of cytoreductive nephrectomy (CNx) on the outcomes in patients with metastatic renal collecting duct carcinoma (CDC). It found that CNx improved the prognosis of metastatic CDC patients, and surgical resection of visible lesions and suitable chemotherapy were identified as alternative treatment strategies.
SCIENTIFIC REPORTS
(2022)
Article
Physiology
Euijung Park, Chin-Rang Yang, Viswanathan Raghuram, Venkatesh Deshpande, Arnab Datta, Brian G. Poll, Kirby T. Leo, Hiroaki Kikuchi, Lihe Chen, Chung-Lin Chou, Mark A. Knepper
Summary: Vasopressin regulates renal water excretion by controlling aquaporin-2 (AQP2) through phosphorylation changes in collecting duct cells. This review identifies 51 vasopressin-regulated phosphorylation sites in 45 proteins and emphasizes the importance of these sites in AQP2 regulation. The study provides web pages listing the phosphorylation sites and functions of the targeted phosphoproteins, suggesting a central role for protein kinase A (PKA) in vasopressin signaling. Other kinases, such as ERK1/2, Camkk2, Cdk18, Erbb3, Mink1, and Src, may also be involved in vasopressin-induced phosphorylation changes. Mapping the regulated phosphoproteins to various processes offers opportunities for future studies on vasopressin-mediated control of AQP2.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2023)
Article
Physiology
Juliano Zequini Polidoro, Nancy Amaral Reboucas, Adriana Castello Costa Girardi
Summary: The study demonstrates that ATRAP attenuates Ang II-induced inhibition of ROMK in collecting duct cells by reducing c-Src activation and blocking ROMK internalization.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hamit Hakan Armagan, Mustafa Naziroglu
Summary: The study found that maintaining GSH homeostasis is crucial for quenching oxidative stress in cortical collecting duct cells and is equally important for modulating TRPM2 activation. Suppressing apoptosis and mitochondrial oxidative stress responses elicited by the oxidant action of GSH depletion is essential.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Biochemical Research Methods
Bjorn Reusch, Malte P. Bartram, Claudia Dafinger, Nicolas Palacio-Escat, Andrea Wenzel, Robert A. Fenton, Julio Saez-Rodriguez, Bernhard Schermer, Thomas Benzing, Janine Altmueller, Bodo B. Beck, Markus M. Rinschen
Summary: Mutations in the MAGED2 gene can cause Bartter Syndrome type 5, characterized by the loss of urinary concentration capability and large urine volumes. The MAGED2 gene is expressed in mouse embryonic kidneys and its knockdown affects protein phosphorylation in kidney cells. This study provides insights into the role of MAGED2 in cellular signalling and potential implications for development and disease.
JOURNAL OF PROTEOMICS
(2022)
Article
Multidisciplinary Sciences
Venkateswara R. Gogulamudi, Danielle Y. Arita, Camille R. T. Bourgeois, Justine Jorgensen, Jing He, William C. Wimley, Ryosuke Satou, Alexis A. Gonzalez, Minolfa C. Prieto
Summary: Evidence suggests that high glucose conditions stimulate the translocation of PRR to the plasma membrane in CD cells, leading to activation of downstream fibrotic factors. This may represent a novel mechanism underlying the development of tubulointerstitial fibrosis in diabetic kidneys.
SCIENTIFIC REPORTS
(2021)
Article
Physiology
Ali Sassi, Yubao Wang, Alexandra Chassot, Isabelle Roth, Suresh Ramakrishnan, Valerie Olivier, Olivier Staub, Khalil Udwan, Eric Feraille
Summary: Fine tuning of Na+ reabsorption occurs in the aldosterone-sensitive distal nephron, primarily regulated by aldosterone through ENaC and Na+ -K+/ -ATPase. Paracellular ion permeability is dependent on tight junctions, particularly claudin-8. Aldosterone coordinately regulates the expression of transcellular Na+ transporters and paracellular claudin-8. Multiple protein kinases, including GSK-3, Lyn, and Abl, mediate the aldosterone-induced upregulation of claudin-8 expression.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Oncology
Qingqiang Zhu, Jun Ling, Jing Ye, Wenrong Zhu, Jingtao Wu, Wenxin Chen
Summary: This study characterized the clinical and CT imaging features of 33 patients with CRCC and 13 patients with CCDC. The distinguishing features of CRCC and CCDC on CT and MR images included differences in calcifications, capsule signs, metastasis, and signal intensity, which may aid in differentiation between the two renal tumor types.
Article
Biochemistry & Molecular Biology
Wouter H. van Megen, Esra Canki, Vera H. A. Wagenaar, Charlotte R. M. M. van Waes, Dorien J. M. Peters, Jenny Van Asbeck-Van der Wijst, Joost G. J. Hoenderop
Summary: The flow rate of pro-urine in the kidney can activate mechanosensitive pathways, leading to ATP release. This study investigates the mechanisms underlying flow-induced ATP release in the collecting duct and its relation to ADPKD.
Article
Biochemistry & Molecular Biology
Gennara Cavallaro, Carla Sardo, Emanuela Fabiola Craparo, Gaetano Giammona
Summary: A novel approach of formulating efficient polymeric carriers based on INU-IMI-DETA was investigated, resulting in improved structural, functional, and biological properties. By chemically modifying with EGF and PEG, two new derivatives were synthesized to enhance the performance of inulin complex nanoaggregates (ICONs). The EGF-decorated complexes showed enhanced siRNA complexation and MCF-7 cell internalization, demonstrating a targeting effect. Moreover, complexes containing both EGF and PEG promoted siSUR internalization, increasing sensitivity to anticancer agents.
Article
Medical Laboratory Technology
Marcus R. Orzabal, Emilie R. Lunde-Young, Josue I. Ramirez, Selene Y. F. Howe, Vishal D. Naik, Jehoon Lee, Cristine L. Heaps, David W. Threadgill, Jayanth Ramadoss
TRANSLATIONAL RESEARCH
(2019)
Article
Public, Environmental & Occupational Health
Artem S. Rogovskyy, David W. Threadgill, Igor A. Akimov, Igor V. Nebogatkin, Yuliya V. Rogovska, Maria V. Melnyk, Sergii P. Rogovskyy
VECTOR-BORNE AND ZOONOTIC DISEASES
(2019)
Article
Multidisciplinary Sciences
Jaye Sudweeks, Brandon Hollingsworth, Dimitri Blondel, Karl J. Campbell, Sumit Dhole, John D. Eisemann, Owain Edwards, John Godwin, Gregg R. Howald, Kevin P. Oh, Antoinette J. Piaggio, Thomas A. A. Prowse, Joshua Ross, J. Royden Saah, Aaron B. Shiels, Paul Q. Thomas, David W. Threadgill, Michael R. Vella, Fred Gould, Alun L. Lloyd
SCIENTIFIC REPORTS
(2019)
Article
Genetics & Heredity
Anika C. Bissahoyo, Yuying Xie, Lynda Yang, R. Scott Pearsall, Daekee Lee, Rosemary W. Elliott, Peter Demant, Leonard McMillan, Fernando Pardo-Manuel de Villena, Joe M. Angel, David W. Threadgill
Article
Cell Biology
Adam Price, Atsushi Okumura, Elaine Haddock, Friederike Feldmann, Kimberly Meade-White, Pryanka Sharma, Methinee Artami, W. Ian Lipkin, David W. Threadgill, Heinz Feldmann, Angela L. Rasmussen
Article
Multidisciplinary Sciences
Raena Eldridge, Daniel Osorio, Katia Amstalden, Caitlin Edwards, Colin R. Young, James J. Cai, Kranti Konganti, Andrew Hillhouse, David W. Threadgill, C. Jane Welsh, Candice Brinkmeyer-Langford
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Tiffany A. Garbutt, Kranti Konganti, Thomas Konneker, Andrew Hillhouse, Drake Phelps, Alexis Jones, David Aylor, David W. Threadgill
Article
Endocrinology & Metabolism
Anna C. Salvador, Danny Arends, William T. Barrington, Ahmed M. Elsaadi, Gudrun A. Brockmann, David W. Threadgill
Summary: Genetic mapping revealed QTLs on Chromosome 1, 5, and 7 associated with fat and lean mass gain, as well as a significant QTL on Chromosome 1 linked to serum HDL cholesterol concentration. Causal network analysis showed a connection between HDL cholesterol and fat mass gain with Fmgq1, while strong sex effects were identified at Fmgq2 and Lmgq1, influenced by diet.
INTERNATIONAL JOURNAL OF OBESITY
(2021)
Article
Infectious Diseases
Leta Elias, John C. Blazier, Yuliya Rogovska, Kranti Konganti, Jiangli Wang, Shuling Liu, Kelley M. Thieman Mankin, Igor Nebogatkin, David W. Threadgill, Artem S. Rogovskyy
Summary: Recent studies have shown significant differences in the microbiome of Dermacentor reticulatus ticks between different geographical regions and sexes.
TICKS AND TICK-BORNE DISEASES
(2021)
Article
Genetics & Heredity
Carolina Mantilla Rojas, Michael P. McGill, Anna C. Salvador, David Bautz, David W. Threadgill
Summary: This study highlights the discrepancy between pre-clinical efficacy and clinical translation of targeted cancer therapies and the significant impact of genetic background on the response in mouse colorectal cancer models. The genetic background dependency of ERBB3 has implications for polyp growth in the intestines, with implications for the use of ERBB3 inhibitors in colorectal cancer treatment.
Review
Environmental Sciences
Danila Cuomo, Margaret J. Foster, David Threadgill
Summary: Lead poisoning is a significant public health concern in environmental justice communities and developing countries. Individual susceptibility to lead exposure varies due to genetic and epigenetic factors. Current studies have provided inconsistent evidence on the identification of susceptibility genes or early exposure biomarkers.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Cardiac & Cardiovascular Systems
Hui Yan, Wanbao Yang, Fenghua Zhou, Quan Pan, Kimberly Allred, Clinton Allred, Yuxiang Sun, David Threadgill, David Dostal, Carl Tong, Shaodong Guo
Summary: This study investigates the role of ovaries and ovarian hormone estrogen in cardiac function and energy metabolism. The results show that estrogen can prevent dilated cardiomyopathy, improve cardiac function and energy metabolism, and enhance lifespan. This provides a mechanistic explanation for the gender difference in the incidence of T2D and cardiovascular diseases.
CIRCULATION-HEART FAILURE
(2022)
Article
Microbiology
Kristin Scoggin, Jyotsana Gupta, Rachel Lynch, Aravindh Nagarajan, Manuchehr Aminian, Amy Peterson, L. Garry Adams, Michael Kirby, David W. Threadgill, Helene L. Andrews-Polymenis
Summary: Understanding the molecular mechanisms of resistance and tolerance to pathogen infection can help in developing novel interventions. A study on mice infected with Salmonella Typhimurium found distinct phenotypes of delayed susceptible, tolerant, and resistant strains. Tolerant strains showed lower preinfection core body temperatures, rapid disruption of body temperature postinfection, higher neutrophil levels, and more severe tissue damage compared to resistant strains. Gene analysis identified specific genes associated with tolerance and resistance, particularly fibrinogen genes.
Article
Multidisciplinary Sciences
Luke Gierus, Aysegul Birand, Mark D. Bunting, Gelshan I. Godahewa, Sandra G. Piltz, Kevin P. Oh, Antoinette J. Piaggio, David W. Threadgill, John Godwin, Owain Edwards, Phillip Cassey, Joshua V. Ross, Thomas A. A. Prowse, Paul Q. Thomas
Summary: This study demonstrates a novel gene drive strategy to eradicate invasive rodent populations on islands by leveraging the super-Mendelian transmission of the t haplotype. Transgenic mice were engineered to validate the effectiveness of this approach.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Yeon Sun Kim, Jia Yuan, Amanda Dewar, Jean-Paul Borg, David W. Threadgill, Xiaofei Sun, Sudhansu K. Dey
Summary: HB-EGF recruits VANGL2 for tyrosine phosphorylation through binding to ERBB2 and ERBB3, thereby influencing uterine epithelial cell polarity and regulating the implantation process.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)