Article
Chemistry, Multidisciplinary
Sohyung Lee, Joseph de Rutte, Robert Dimatteo, Doyeon Koo, Dino Di Carlo
Summary: Researchers have developed a scalable method for manufacturing hydrogel microparticles with defined shapes and chemical functionalization. The process involves using a two-phase system and microfluidic technology, and allows for localized surface chemistry on the microparticles. These microparticles can be used for cell loading and single-cell secretion analysis.
Article
Cardiac & Cardiovascular Systems
William Bracamonte-Baran, Nisha A. Gilotra, Taejoon Won, Katrina M. Rodriguez, Monica Talor, Byoung C. Oh, Jan Griffin, Ilan Wittstein, Kavita Sharma, John Skinner, Roger A. Johns, Stuart D. Russell, Robert A. Anders, Qingfeng Zhu, Marc K. Halushka, Gerald Brandacher, Daniela Cihakova
Summary: The study found that myeloid cells dominate the graft leukocyte compartment in heart transplant patients, with variable CD4:CD8 T-cell ratios. PD-L1 expression in graft endothelial cells, fibroblasts, and myeloid leukocytes varied from negligible to 60%. There was a significant inverse correlation between PD-L1(+)HLA-DR+ endothelial cells and CD8(+) T cells, and these patterns were independent of demographic, clinical, and hemodynamic parameters. Additionally, a murine model confirmed the importance of endothelial PD-L1 expression in regulating CD8(+) T-cell infiltration and potential alloresponses in heart transplantation.
CIRCULATION-HEART FAILURE
(2021)
Article
Immunology
Zhizhong Ren, Yuanxun Yue, Yuewei Zhang, Jiahong Dong, Ying Liu, Xiaowei Yang, Xin Lin, Xueqiang Zhao, Zhanqi Wei, Yu Zheng, Tianxiao Wang
Summary: The study demonstrated that m-TACE has a positive regulatory effect on the anticancer immune function of HCC patients and can be used in combination with immune adjuvant therapies to enhance the efficacy of HCC treatment.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Suiane Cavalcante, Sofia Viamonte, Rui S. Cadilha, Ilda P. Ribeiro, Ana Cristina Goncalves, Joao Sousa-Venancio, Marisol Gouveia, Manuel Teixeira, Mario Santos, Jose Oliveira, Fernando Ribeiro
Summary: This study aims to evaluate the effects of a single exercise bout on the circulating levels of EPCs and CECs in heart failure patients. The results showed that a single exercise bout increased the levels of EPCs but didn't change the levels of CECs. Heart failure patients had lower levels of EPCs compared to the age-matched group, but the exercise bout restored their EPCs levels to a level similar to the age-matched group.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Review
Biochemical Research Methods
Kai Pang, Sihan Dong, Yuxi Zhu, Xi Zhu, Quanyu Zhou, Bobo Gu, Wei Jin, Rui Zhang, Yuting Fu, Bingchen Yu, Da Sun, Duanmu Zheng, Xunbin Wei
Summary: Flow cytometry is a versatile tool for detecting and measuring characteristics of cells or particles. Recent advancements in techniques such as in vivo photoacoustic FC, coherent Raman FC, and microfluidic FC have expanded the applications of FC in basic research and clinical practice.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Biochemical Research Methods
Dmitry I. I. Strokotov, Vyacheslav M. M. Nekrasov, Konstantin V. V. Gilev, Andrey A. A. Karpenko, Valeri P. P. Maltsev
Summary: The performance of ultraviolet light scattering (UVLS) in the analysis of individual particles with flow cytometry is demonstrated for the first time. UVLS offers improved analysis of submicron particles due to its dependence on the wavelength of the incident light. A scanning flow cytometer (SFC) is used to analyze submicron particles and solve the inverse light-scattering problem to retrieve particle characteristics. UVLS successfully characterizes standard polystyrene microspheres and has potential applications in the analysis of chylomicrons (CMs) in serum.
Article
Biochemical Research Methods
Jessie E. Arce, Joshua A. Welsh, Sean Cook, John Tigges, Ionita Ghiran, Jennifer C. Jones, Andrew Jackson, Matthew Roth, Aleksandar Milosavljevic
Summary: The NanoFlow Repository, the first implementation of the MIFlowCyt-EV framework, has been developed to meet the need for data sharing and minimum reporting standards for extracellular particles (EPs).
Article
Biochemical Research Methods
Paul H. Dannenberg, Jisoo Kang, Nicola Martino, Anokhi Kashiparekh, Sarah Forward, Jiamin Wu, Andreas C. Liapis, Jie Wang, Seok-Hyun Yun
Summary: This article demonstrates a cell barcode technology using laser particles, which can be used for cell sorting and routing through a microfluidic platform integrated with a high-speed spectrometer. By utilizing the spectral emission of laser particles, individual cells labeled with different laser colors can be distinguished and sorted.
Article
Hematology
Laura Somodi, Ildiko Beke Debreceni, Greta Kis, Marco Cozzolino, Janos Kappelmayer, Miklos Antal, Gyorgy Panyi, Helga Bardos, Nicola J. Mutch, Laszlo Muszbek
Summary: Receptor-mediated activation can expose cFXIII on the surface of more than 60% of platelets, while non-receptor mediated activation failed to do so, indicating that the translocation of cFXIII requires additional receptor-mediated mechanisms.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Chemistry, Analytical
Gugu Kubheka, Estela Climent, Charlie Tobias, Knut Rurack, John Mack, Tebello Nyokong
Summary: This study used azaBODIPY dyes doped into polystyrene particles as optical probes for the detection of high-risk HPV types. The particles were successfully attached with HPV DNA single strands, and demonstrated low detection limits and a narrow detection range for HPV11. The results suggest that these particles have the potential for multiplex assays and further study.
Article
Medical Laboratory Technology
Wouter H. G. Hubens, Tiago Maie, Matthis Schnitker, Ledio Bocova, Deepika Puri, Martina Wessiepe, Jan Kramer, Lothar Rink, Steffen Koschmieder, Ivan G. Costa, Wolfgang Wagner
Summary: Cell-type specific DNA methylation can be used to accurately count different leukocyte subsets in blood. In this study, the authors developed targeted DNAm assays and used digital droplet PCR to measure DNAm levels in venous blood and dried blood samples. The results showed good correlations between epigenetic estimates and conventional cell counting methods, and dried blood samples facilitated self-sampling for easier testing accessibility.
CLINICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Carolus Vitalis, Tobias Wenzel
Summary: Microfluidic droplet screens are an innovative platform for high-throughput biotechnology, contributing to advancements in discovery, product optimization, and analysis. This review focuses on the emerging trends of interaction assays in microfluidic droplets, highlighting their unique suitability. These assays encompass various biological entities and demonstrate versatility and scope. Recent advancements have expanded the scale of bioanalysis and biotechnological product design. Additionally, the review discusses pioneering breakthroughs in extending droplet-based screens into new domains, such as cargo delivery within human bodies and the development of responsive droplet structures.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Chengxin Wu, Xing Wei, Xue Men, Xuan Zhang, Yong-Liang Yu, Zhang-Run Xu, Ming-Li Chen, Jian-Hua Wang
Summary: CytoLM is a two-dimensional cytometry platform developed for single-particle and single-cell sampling and analysis, featuring high sensitivity and high temporal resolution. It combines VCCS-LIF and ICP-MS technologies to enable high-throughput, multi-dimensional, and multi-parameter characterization of particles and cells, showing vast potential in life science analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Ecology
Peter Rubbens, Ruben Props, Frederiek-Maarten Kerckhof, Nico Boon, Willem Waegeman
Summary: This study focuses on the potential of flow cytometry in diagnosing microbiome-associated diseases like Crohn's disease. Reanalyzing a disease cohort characterized by both 16S rRNA gene analysis and flow cytometry, changes in microbial community structure were found to be reflected in both types of data. Cytometric fingerprints can serve as a rapid diagnostic tool for classifying samples based on CD state.
Article
Chemistry, Analytical
Li Wu, Shihan Xu, Jingang Wang, Amy L. Paguirigan, Jerald P. Radich, Yuling Qin, Daniel T. Chiu
Summary: Researchers have developed a single cell dispenser inspired by electrohydrodynamic jet printing, which can be used for single-cell multiomics studies, achieving accurate droplet generation and single-cell dispensing through systematic parameter optimization.
ANALYTICAL CHEMISTRY
(2021)