期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 25, 期 7, 页码 1237-1246出版社
SPRINGER
DOI: 10.1007/s13361-014-0882-3
关键词
Imaging mass spectrometry; Lipidomics; Human lung cancer; Xenografts; UHPLC; MALDI
资金
- Basque Government
- Instituto Carlos III, Ministerio de Economia y Competitividad
- INSERM (Institut National de la sante et de la recherche medical)
Human tumor xenografts in immunodeficient mice are a very popular model to study the development of cancer and to test new drug candidates. Among the parameters analyzed are the variations in the lipid composition, as they are good indicators of changes in the cellular metabolism. Here, we present a study on the distribution of lipids in xenografts of NCI-H1975 human lung cancer cells, using MALDI imaging mass spectrometry and UHPLC-ESI-QTOF. The identification of lipids directly from the tissue by MALDI was aided by the comparison with identification using ESI ionization in lipid extracts from the same xenografts. Lipids belonging to PCs, PIs, SMs, DAG, TAG, PS, PA, and PG classes were identified and their distribution over the xenograft was determined. Three areas were identified in the xenograft, corresponding to cells in different metabolic stages and to a layer of adipose tissue that covers the xenograft.
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