4.5 Article

Absorption-Mode Fourier Transform Mass Spectrometry: The Effects of Apodization and Phasing on Modified Protein Spectra

期刊

出版社

AMER CHEMICAL SOC
DOI: 10.1007/s13361-013-0600-6

关键词

Absorption-mode; Top-down; Harmonics; Apodization

资金

  1. University of Warwick, Department of Chemistry
  2. Warwick Centre for Analytical Science (EPSRC) [EP/F034210/1]
  3. EPSRC [EP/F034210/1] Funding Source: UKRI
  4. Engineering and Physical Sciences Research Council [EP/F034210/1] Funding Source: researchfish

向作者/读者索取更多资源

The method of phasing broadband Fourier transform ion cyclotron resonance (FT-ICR) spectra allows plotting the spectra in the absorption-mode; this new approach significantly improves the quality of the data at no extra cost. Herein, an internal calibration method for calculating the phase function has been developed and successfully applied to the top-down spectra of modified proteins, where the peak intensities vary by 100x. The result shows that the use of absorption-mode spectra allows more peaks to be discerned within the recorded data, and this can reveal much greater information about the protein and modifications under investigation. In addition, noise and harmonic peaks can be assigned immediately in the absorption-mode.

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