Article
Biochemical Research Methods
Rebecca L. Cain, Ian K. Webb
Summary: Electrospray ionization mass spectrometry (ESI-MS) experiments were used to study the solution-like structures of intact proteins, specifically by controlling the protein unfolding process and analyzing folding intermediates. By mixing various pH solutions online with aqueous cytochrome C, the changes in charge states, arrival time distributions (ATDs), and electron capture dissociation (ECD) were measured to detect folding intermediates and unfolded protein structures.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Optics
Hien T Nguyen, Kim-Ngan H Nguyen, Ngoc-Loan Phan, Cam-Tu Le, DinhDuy Vu, Lan-Phuong Tran, Van-Hoang Le
Summary: This study demonstrates the nonperturbative role of dynamic core-electron polarization (DCeP) in high-order harmonic generation (HHG) of the CO molecule. The inclusion of DCeP results in a significant change in the even-to-odd ratio of harmonic intensities, making the theoretically predicted values consistent with experimental data. These findings establish the importance of incorporating DCeP into the standard framework for strong-field physics.
Article
Biochemistry & Molecular Biology
Ioana Cezara Caba, Raluca Stefanescu, Bogdan Ionel Tamba
Summary: This study used mass spectrometry to investigate the formation of noncovalent complexes between oleuropein and amyloid beta. By modifying the chemical environment and conducting proteolytic experiments, the binding site was identified. The results highlight the importance of further research on the effect of oleuropein against amyloid beta aggregation.
Article
Chemistry, Analytical
Qiong Liang, Siyu Liu, Wei Xu, Yanbing Zhai
Summary: In this study, a capillary-in-capillary electrospray ionization (CC-ESI) source was developed for spontaneous liquid sampling and quantitative analysis based on the capillary phenomenon and electrospray ionization mass spectrometry (ESI-MS). The CC-ESI had advantages of cheap price, high portability, disposability, quantitation capability, and adequate sensitivity.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Chen Ling, Qiaofang Shi, Zhanpeng Wei, Jingjing Zhang, Junjie Hu, Jiying Pei
Summary: A rapid, environmentally friendly wooden-tip electrospray ionization mass spectrometry (ESI-MS) method was introduced for the determination of quinones in PM2.5, urine, and serum, showing high sensitivity and applicability in complex matrices without extensive sample preparation (LOD range: 0.22-1.48 ng).
Review
Pharmacology & Pharmacy
Manman Qin, Yuqing Qian, Lu Huang, Chao Zhong, Mingdong Li, Jun Yu, Huanwen Chen
Summary: Extraction electrospray ionization mass spectrometry (EESI-MS) enables effective ionization of trace molecules in complex samples without sample pretreatment, providing high sensitivity, selectivity, and response speed. It also allows for advanced functionalization of high-energy molecular species. This article systematically reviews the effects of operating conditions on the analytical performance of EESI-MS for various pharmaceutical compounds and introduces its potential application in molecular functionalization for drug discovery.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Analytical
C. Thoben, C-R Raddatz, M. Lippmann, Z. Salehimoghaddam, S. Zimmermann
Summary: This study investigated the performance of an electrospray ionization ion mobility spectrometer by varying the electric field strength in the desolvation region, introducing a new tristate ion shutter to enhance sensitivity for ions with higher molecular mass and lower ion mobility. The optimal ratio of electric field strengths in the drift and desolvation region was found to be affected by the electric field in the tristate ion shutter. The solvent flow rate also needs to be considered when setting the field strengths in the desolvation region to maintain proper electrospray function.
Article
Chemistry, Analytical
Madison E. Pursell, Daud Sharif, Anthony DeBastiani, Chong Li, Sandra Majuta, Peng Li, Stephen J. Valentine
Summary: The new ionization technique, vibrating sharp-edge spray ionization (cVSSI), coupled with corona discharge, has been investigated for its atmospheric pressure chemical ionization (APCI) capabilities. The optimized source has shown improved ion signal intensity, overcoming of matrix effects, and limitation of ion suppression, compared to the state-of-the-art ESI source and a new APCI-like source. The results demonstrate significant enhancements in ion signal intensity and abilities to overcome ion suppression in both methanol and aqueous samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
J. Michael Sutton, Noha M. El Zahar, Michael G. Bartlett
Summary: The study focuses on improving the mobile phase of electrospray oligonucleotides to reduce spectral complexity and increase sensitivity. By using different combinations of fluorinated alcohols and alkylamines, it is possible to selectively reduce the charge states of oligonucleotides. Additionally, anionic salts are used to stabilize complexes and provide unique structural information about the molecules.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2021)
Article
Biochemical Research Methods
Hiromori Murashima, Akimasa Fujihara
Summary: The study investigated chiral recognition between amino acids and monosaccharides in the gas phase as a model for chemical evolution in interstellar molecular clouds. Results showed that the optical properties of Trp in the clusters induced by 285-nm photoexcitation were useful for identifying and quantifying pentose enantiomers. Additionally, pentose enantiomeric excess in solution could be determined from relative abundances observed in a single product ion spectrum of 285-nm photoexcited hydrogen-bonded clusters of H+(L-Trp) and pentose.
ANALYTICAL BIOCHEMISTRY
(2021)
Review
Chemistry, Analytical
Bin Hu, Zhong-Ping Yao
Summary: This article reviews the technical aspects and important applications of wooden-tip ESI-MS in various fields. Wooden-tip ESI-MS has shown significant achievements in the past 10 years and has the potential for further development and application.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biotechnology & Applied Microbiology
Niklas Biere, Dennis Kreft, Volker Walhorn, Sabrina Schwarzbich, Thorsten Glaser, Dario Anselmetti
Summary: Dinuclear copper complexes have been designed to selectively bind to neighboring phosphate moieties in DNA. Previous works have investigated the effects of these complexes on DNA through biophysical, biochemical, and cytotoxic analyses, with atomic force microscopy (AFM) being particularly valuable in assessing the macroscopic properties of the DNA strands. To gain a deeper understanding of these effects, cutting-edge preparation methods and scanning force microscopy under ultra-high vacuum (UHV) conditions were used to obtain submolecular resolution images. The mechanics and interactions of the DNA strands were analyzed at the single base pair level, including the formation of melting bubbles and the interaction of individual complex molecules.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Michael Goetze, Lukasz Polewski, Leila Bechtella, Kevin Pagel
Summary: This article introduces a 3D-printable nano-electrospray ionization (nano-ESI) source for Bruker instruments, which can be assembled by anyone with access to a 3D printer. The source can be fitted to any Bruker mass spectrometer with an ionBooster ESI source and only requires minor, reversible changes to the original Bruker hardware. The general utility of the source was demonstrated by recording high-resolution MS spectra of small molecules, intact proteins, as well as complex biological samples in negative and positive ion mode on two different Bruker instruments.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Multidisciplinary Sciences
Amanda Gisler, Kapil Dev Singh, Jiafa Zeng, Martin Osswald, Mo Awchi, Fabienne Decrue, Felix Schmidt, Noriane A. Sievi, Xing Chen, Jakob Usemann, Urs Frey, Malcolm Kohler, Xue Li, Pablo Sinues
Summary: Exhaled breath analysis provides valuable molecular-level information and has promising potential for precision medicine. However, the lack of validation in multicenter trials hinders the transition of breath tests into routine clinical practice. In this study, an interoperability framework was developed and applied, revealing a technical variability of approximately 20% and identifying a core signature of the human exhaled metabolome. The results showed high inter-subject variability for certain metabolic classes but relative stability in other regions, such as the TCA cycle. This framework and the overview of metabolic coverage will pave the way for future large-scale multicenter trials.
Article
Chemistry, Analytical
Ziqi Fang, Yanfen Xu, Lian Duan, Yu Wei, Bo Hong, Ying Lv, Bo Yuan, Wen Liu, Guoxing Jing, Wenshan Li, Wenjie Liu, Jianna Yu
Summary: In this study, a method using ammonium acerate and secondary electrospray ionization ion mobility spectrometry (SESI-IMS) was evaluated for rapid determination of volatile biogenic amine contents in grass carp. The method showed minimum sample preparation and interference, and volatile biogenic amines displayed unique drift times without overlapping. The content of volatile biogenic amines correlated well with storage times and other freshness indicators.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemical Research Methods
Liqing Yao, Yunfeng Chai, Cuirong Sun, Yuanjiang Pan
JOURNAL OF MASS SPECTROMETRY
(2015)
Article
Biochemical Research Methods
Yunfeng Chai, Xingchuang Xiong, Lei Yue, You Jiang, Yuanjiang Pan, Xiang Fang
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2016)
Article
Agriculture, Multidisciplinary
Pingxiang Liu, Hongping Chen, Guanwei Gao, Zhenxia Hao, Chen Wang, Guicen Ma, Yunfeng Chai, Lin Zhang, Xin Liu
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2016)
Article
Biochemical Research Methods
Yunfeng Chai, Lu Wang, Lin Wang
JOURNAL OF MASS SPECTROMETRY
(2016)
Article
Chemistry, Multidisciplinary
Xuan-Feng Jiang, Hui Huang, Yun-Feng Chai, Tracy Lynn Lohr, Shu-Yan Yu, Wenzhen Lai, Yuan-Jiang Pan, Massimiliano Delferro, Tobin J. Marks
Article
Biochemical Research Methods
Yunfeng Chai, Guanwei Gao, Shanshan Shen, Xin Liu, Chengyin Lu
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2017)
Article
Biochemical Research Methods
Yunfeng Chai, Yunlong Shao, Lu Wang, Lin Wang
JOURNAL OF MASS SPECTROMETRY
(2017)
Article
Biochemical Research Methods
Yunfeng Chai, Hongping Chen, Xin Liu, Chengyin Lu
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2018)
Article
Biochemical Research Methods
Zhen Liu, Yunlong Shao, Guizhen Zhu, Xiayan Wang, Yunfeng Chai, Lin Wang
JOURNAL OF MASS SPECTROMETRY
(2019)
Article
Biochemical Research Methods
Yunfeng Chai, Hongping Chen, Guanwei Gao, Xin Liu, Chengyin Lu
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2019)
Article
Biochemical Research Methods
Yunfeng Chai, Hongping Chen, Xin Liu, Chengyin Lu
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2019)
Letter
Biochemical Research Methods
Yunfeng Chai, Lu Wang, Chengyin Lu
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2020)
Article
Biochemical Research Methods
Yunfeng Chai, Hongping Chen, Chengyin Lu
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2020)
Article
Multidisciplinary Sciences
Shanshan Shen, Yunfeng Chai, Qirong Shen, You Jiang, Xiang Fang, Yuanjiang Pan
Summary: This study reports the gas-phase amination reaction of aromatic hydrocarbons under corona discharge conditions with N-2 gas as the nitrogen source. Unusual product ions were observed and identified through high resolution MS and tandem mass spectrometric analysis. The findings provide insights into a potential mechanism for synthesizing anilines in a novel and environmentally friendly manner.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Lu Wang, Yunfeng Chai, Wenquan Zhu, Yuanjiang Pan, Cuirong Sun, Su Zeng