Article
Energy & Fuels
Jianxun Wu, Han Li, Qianhui Zhao, Bing Zhou, Zengmin Lun, Yahe Zhang, Keng H. Chung, Quan Shi
Summary: This study separated natural interfacial materials (IMs) from crude oils in different regions of China using the wet silica adsorption method. High resolution mass spectrometry was used to reveal the functional groups of the separated IMs. The composition of IMs varied greatly among different crude oils, including both naturally occurring compounds and artificially introduced chemicals.
JOURNAL OF PETROLEUM SCIENCE AND ENGINEERING
(2022)
Article
Chemistry, Analytical
Oliver J. Hale, James W. Hughes, Emma K. Sisley, Helen J. Cooper
Summary: This study demonstrates the detection and identification of a range of intact endogenous protein assemblies with various stoichiometries from different tissue types using multiple NAMS techniques, showing a greater than twofold increase in accessible molecular weight. Additionally, spatial distributions of protein assemblies up to 94 kDa were mapped in brain and kidney by nanospray desorption electrospray ionization (nano-DESI) mass spectrometry imaging.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Ivan Mauricio Huerfano Barco, Julio Cesar Espana Amortegui, Jairo Arturo Guerrero Dallos
Summary: The qualitative screening and quantitative determination of 201 pesticides were optimized using design of experiments. The study utilized an upgradeable database for post-targeted screening and performed standard-free quantitation by LC-HRMS. Fine tuning of instrumental parameters, including HESI conditions, sample composition, and MS/MS acquisition modes, showed improved signal intensity and shape for most compounds.
Article
Chemistry, Analytical
Xue-Lian Jiang, Yao Zhu, Gai-Fan Ma, Ping Liu, Lin-Lin Chen
Summary: The overall chemical profile of Renshen-Yangrong Pill (RYP), a traditional Chinese medicine, was investigated using UPLC-LTQ/Orbitrap/MS, and the quantitative distributions of representative components in the preparation were obtained. A total of 132 components were identified or characterized, with hesperidin and paeoniflorin being the most abundant constituents. This study provides valuable insights into the chemical composition of RYP and lays a foundation for further exploration of its effective components and quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemical Research Methods
Keenan Pearson, Caroline Doherty, Drason Zhang, Nicole A. Becker, L. James Maher
Summary: This article describes the challenges encountered when using standard qPCR methods for quantitating random DNA libraries flanked by fixed sequence regions, and proposes simple solutions. The study shows that incomplete renaturation of PCR products containing random regions occurs in late PCR cycles when extension fails due to reagent depletion, resulting in loss of intercalator fluorescence. By adjusting the step at which intercalator fluorescence is monitored, accurate qPCR analysis of random DNA libraries can be achieved.
ANALYTICAL BIOCHEMISTRY
(2022)
Review
Chemistry, Multidisciplinary
Juanning Ren, Rongrong Wang, Wenjing Fan, Tong Li, Pengzhi Dong, Guixiang Pan, Ming Ren, Miaomiao Jiang
Summary: Xin-Su-Ning Capsules (XSNC) is an effective prescription for arrhythmia composed of eleven Chinese herbs. Gas chromatography-mass spectrometry (GC-MS) and ultra high-performance liquid chromatography quadrupole electrostatic orbitrap high resolution mass spectrometry (UHPLC-Q-Exactive-Orbitrap-MS) were used to identify the chemical profile of XSNC, while a rapid UHPLC-QQQ-MS/MS method was developed for quality evaluation. The study identified multiple compounds in XSNC and validated the quantitative analysis method for sixteen components with satisfactory results in terms of linearity, precision, and accuracy.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Applied
Ping Qi, Qingqiong Zhou, Guiyun Chen, Zihao Lin, Jinli Zhao, Huiying Xu, Hui Gao, Donghao Liu, Xinwu Mao
Summary: Public exposure to synthetic dyes through foods has raised continuous and serious concerns. A method for simultaneous screening and quantitation of fat-soluble synthetic dyes in food has been developed and validated, using C-18 d-SPE clean-up and UHPLC-Q-Orbitrap HRMS on Full-MS/dd-MS2 mode. The method can distinguish 104 dyes, including 6 pairs of isomers, based on chromatographic separation and unique product ions. The validated method has been successfully applied to real commercial food samples, revealing the presence of two selected illegal dyes in three samples.
Article
Food Science & Technology
An Du, Wei Jia
Summary: A customized short-chain peptidomics approach was used to screen and analyze potential short-chain peptides in Feng-flavor Baijiu. Thirty-nine characteristic peptides were identified and accurately quantified. Based on a quantitative structure-activity relationship (QSAR) model, four novel antioxidant peptides with high ABTS radical scavenging capacity were obtained. These peptides were found to inhibit the Keap-Nrf2 interaction and promote the accumulation of Nrf2. This study provides insights into functional peptides in Baijiu and the aging mechanism.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Sebastian Imperiale, Ksenia Morozova, Giovanna Ferrentino, Matteo Scampicchio
Summary: This work developed an optimized workflow for targeted analysis of triacylglycerols (TAGs) in milk using liquid chromatography coupled with a Q-Exactive Orbitrap mass spectrometer. The optimized parameters included resolution, automatic gain control target, and flow rate. The optimized conditions were used to successfully identify up to 14 target triglycerides in milk fat extracts, providing helpful information for MS-based lipidomic workflows and targeted milk lipid analysis.
FOOD ANALYTICAL METHODS
(2022)
Article
Biochemical Research Methods
Maoyuan Jiang, Shiyao Hua, Liang Zou, Yitao Wang, Peng Li
Summary: In this study, a method based on online two-dimensional hydrophilic interaction and reversed-phase liquid chromatography coupled with hybrid linear ion trap-Orbitrap mass spectrometry was proposed and used to characterize the chemical profile of Huangqi Guizhi Wuwu decoction. By optimizing trap columns and online dilution, potential analyte loss was decreased and system resolution was improved. A total of 170 chemical constituents were identified or tentatively characterized in both positive and negative ion modes.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yechen Hu, Bo Jiang
Summary: The EnaBe strategy was developed to overcome the challenges in identifying N-phosphorylation status. By utilizing SiO2@DpaZn microspheres and beta-elimination treatment, the strategy efficiently enriched and enhanced the mass spectrometry signals of N-phosphopeptides. As a result, 16 N-phosphoproteins in E. coli were successfully identified.
Article
Biochemical Research Methods
Kevan T. Knizner, Michael C. Bagley, Kenneth P. Garrard, Jan-Peter Hauschild, Fan Pu, Nathaniel L. Elsen, Jon D. Williams, David C. Muddiman
Summary: Infrared matrix-assisted laser desorption ionization (IR-MALDESI) combines the advantages of electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI) and is a valuable tool for high-throughput screening (HTS) analysis. The optimized timing of the C-trap in IR-MALDESI can reduce ambient ion accumulation and maximize analyte ion accumulation. Additionally, this method can be used to measure intact antibodies in large molecules.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Food Science & Technology
Qian-qian Liu, Bin Li, Kang Sun, Cai-hong Li, Nuzhat Shehla, Yu-pei Yang, Liang Cao, Wei Wang, Rong-xia Liu
Summary: A novel UHPLC-Q-Orbitrap HRMS method was established for the simultaneous quali-quantitative analysis of 204 compounds in Kadsura heteroclita stem, including triterpenoids, lignans, sesquiterpenes, fatty acids, phenolic acids, and flavonoids. Twelve representative bioactive components were successfully quantified using this method, which was fully validated and can be applied to quantify more active components of KHS in the future.
JOURNAL OF FOOD AND DRUG ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Seol Jang, Ami Lee, Youn-Hwan Hwang
Summary: In this study, a method for analyzing the components of Oryeong-san quantitatively and qualitatively was established. Ultra-high-performance liquid chromatography/quadrupole Orbitrap mass spectrometry was used to profile the components, and a quantitative method based on ultra-high-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry was established to simultaneously measure the identified compounds. The method was validated and can be used for quality assessment and management of Oryeong-san.
Article
Nanoscience & Nanotechnology
Sen Zhang, Jia-yuan Li, Wei Gao, Jun-qin Qiao, Hong-zhen Lian
Summary: Ti3C2-Fe3O4 composites with exposed non-modified Ti3C2 MXene nanosheets were designed and prepared for the enrichment of phosphopeptides. The composites interacted with the phosphopeptides through metal oxide affinity chromatography and hydrophilic interaction chromatography mechanisms. The magnetic nanomaterial showed high sensitivity and selectivity, and satisfactory results were obtained in the enrichment of phosphopeptides from real samples and traditional Chinese medicine injection.
ACS APPLIED MATERIALS & INTERFACES
(2023)