期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 20, 期 4, 页码 667-673出版社
SPRINGER
DOI: 10.1016/j.jasms.2008.12.003
关键词
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资金
- National Cancer Institute [R33 CA-105295]
- W.M. Keck Foundation
- William R. Kenan, Jr. Fund for Engineering, Technology Science
- North Carolina State University
- National Science Foundation [CHE041-5360]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [0848319] Funding Source: National Science Foundation
We report the implementation of an infrared laser onto our previously reported matrix-assisted laser desorption electrospray ionization (MALDESI) source with ESI post-ionization yielding multiply charged peptides and proteins. Infrared (IR)-MALDESI is demonstrated for atmospheric pressure desorption and ionization of biological molecules ranging in molecular weight from 1.2 to 17 kDa. High resolving power, high mass accuracy single-acquisition Fourier transform ion cyclotron resonance (FT-ICR) mass spectra were generated from liquid-and solid-state peptide and protein samples by desorption with an infrared laser (2.94 mu m) followed by ESI post-ionization. Intact and top-down analysis of equine myoglobin (17 kDa) desorbed from the solid state with ESI post-ionization demonstrates the sequencing capabilities using IR-MALDESI coupled to FT-ICR mass spectrometry. Carbohydrates and lipids were detected through direct analysis of milk and egg yolk using both UV- and IR-MALDESI with minimal sample preparation. Three of the four classes of biological macromolecules (proteins, carbohydrates, and lipids) have been ionized and detected using MALDESI with minimal sample preparation. Sequencing of O-linked glycans, cleaved from mucin using,, reductive beta-elimination chemistry is also demonstrated. (J Am Soc Mass Spectrom 2009, 20, 667-673) (C) 2009 Published by Elsevier Inc. on behalf of American Society for Mass Spectrometry
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