期刊
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
卷 19, 期 7, 页码 1027-1040出版社
AMER CHEMICAL SOC
DOI: 10.1016/j.jasms.2008.03.005
关键词
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资金
- NCRR NIH HHS [P41 RR018942, P41 RR018942-035067, P41 RR018942-01A29004, RR018942, P41 RR018942-028693] Funding Source: Medline
Characterization of structural isomers has become increasingly important and extremely challenging in glycobiology. This communication demonstrates the capability of ion-trap mass spectrometry in conjunction with 157 nm photofragmentation to identify different structural isomers of permethylated N-glycans derived from ovalburnin without chromatographic separation. The results are compared with collision-induced dissociation (CID) experiments. Photodissociation generates' extensive cross-ring fragment ions as well as diagnostic glycosidic product ions that are not usually observed in CID MS/MS experiments. The detection of these product ions aids in characterizing indigenous glycan isomers. The ion trap facilitates MS experiments on the diagnostic glycosidic fragments and cross-ring product ions generated through photofragmentation, thus allowing unambiguous assignment of all of the isomeric structures associated with the model glycoprotein used in this study. Photofragmentation is demonstrated to be a powerful technique for the structural characterization of glycans.
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