Article
Environmental Sciences
Da Huo, Fang Su, Hongsheng Yang, Lina Sun
Summary: This study reported exosomes secreted by sea cucumber Apostichopus japonicus, which were cup-shaped and had an average size of 74.65 nm, and identified specific exosome biomarkers (HSP70, TSG101, and CD9). The global expression of microRNAs in exosomes from normal and heat-stressed A. japonicus was analyzed, revealing increased exosome production and altered expression profiles of microRNAs under heat stress. Several differentially expressed microRNAs were commonly found, indicating their importance in the heat stress response. Target gene analysis revealed their involvement in replication and repair during the initial response and metabolism adjustments during acclimation to high temperature conditions. These findings provide insights into the regulatory roles of exosomes and microRNAs in sea cucumber's response to environmental stresses exacerbated by global warming.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Chenxi Zhang, Libin Zhang, Lingling Li, Mohamed Mohsen, Fang Su, Xu Wang, Chenggang Lin
Summary: This study compared the transcriptomes of sea cucumbers before and after exposure to cadmium (Cd) and/or microplastics (MPs), and investigated the changes in enzyme activity and content of certain compounds. The results showed that high concentration of MPs increased superoxide dismutase (SOD) activity. Combination treatment of high concentration Cd and MPs resulted in significant upregulation of various indicators and a large number of differential expression genes (DEGs). Cd exposure led to the upregulation of a protein associated with apoptosis and inflammation, while MPs exposure resulted in an imbalance of certain inflammatory proteins.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Fisheries
Xiaoshang Ru, Libin Zhang, Shilin Liu, Yunning Jiang, Long Li
Summary: Income breeding strategy allows all adults of sea cucumber to reproduce annually, even individuals with poor body condition and small maternal size. During reproduction, sea cucumbers allocate income resources to the demands of reproduction, growth and storage simultaneously, but income breeding also places a metabolic burden on the breeding adults.
Article
Environmental Sciences
Mohamed Mohsen, Chenggang Lin, Shilin Liu, Hongsheng Yang
Summary: Laboratory experiments showed that microplastics and microfibers can transfer into the body wall of sea cucumbers, entering through the outer surface. In samples collected from the field, 86% of sea cucumbers were found to contain microplastics, mainly in the form of microfibers. The need to control microplastic pollution during the farming and processing of sea cucumbers was emphasized based on the findings of this study.
Article
Environmental Sciences
Nobuharu Inaba, Takuma Matsumoto, Hiroshi Kawai, Yuji Anaguchi, Kohei Matsuno
Summary: The study reveals that the kelp crab Pugettia ferox may cause significant predation mortality on commercially important Japanese sea cucumber Apostichopus japonicus, especially in areas releasing hatchery-produced juveniles. Furthermore, the unique ecological trait of Pugettia ferox utilizing freshly chopped sea cucumbers as decoration material and food storage is worth noting.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Environmental Sciences
Zhonglei Ju, Guoxiang Liao, Yulu Zhang, Nan Li, Xishan Li, Yuhang Zou, Wei Yang, Deqi Xiong
Summary: This study investigated the oxidative stress response to high temperature in the respiratory tree and body wall of the sea cucumber. The results showed that high temperature caused an increase in reactive oxygen species levels and antioxidant activities in the respiratory tree, but no significant changes were observed in the body wall. Lipid peroxidation was observed in both tissues. The respiratory tree was found to be more susceptible to high temperature changes compared to the body wall. These findings provide insights into the oxidative stress response of the sea cucumber to high temperature.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Fisheries
Chong Wang, Wei Wang, Liang Yao, Songwen Sang, Chenghua Li, Qingli Zhang
Summary: CMNV, a covert mortality nodavirus, was found to infect sea cucumber Apostichopus japonicus, causing various pathological lesions in tissues including muscle necrosis. This infection could potentially impact larval rearing and has implications for aquaculture in China. Sea cucumber is an important species in Chinese aquaculture, and further study of CMNV infection in this species is needed to understand the full implications.
Article
Food Science & Technology
Xin Xiong, Wancui Xie, Jingwen Xie, Hang Qi, Xihong Yang, Hongyan Li, Hongxia Che, Lin Song, Xiufang Dong
Summary: By tenderizing Apostichopus japonicus body wall at 37 degrees Celsius, this study found changes in collagen fiber structure, protein oxidation, and degradation, laying the foundation for further research on the tenderization mechanism of sea cucumbers.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Jingjing Zhang, Yeqing Zhou, Luo Wang, Yanxia Liu, Zhiping Lin, Zhenlin Hao, Jun Ding, Yaqing Chang
Summary: This study used metagenome sequencing to analyze the gut microbiota of A. japonicus and found that Bacteroidetes and Proteobacteria were the dominant phyla. The bacterial community in the gut showed asymmetry compared to the surrounding environment, and the gut-colonized bacteria were influenced by the surrounding water and feed, potentially affecting the growth and metabolism of A. japonicus.
Article
Environmental Sciences
Chenxi Zhang, Chenggang Lin, Lingling Li, Mohamed Mohsen, Ting Wang, Xu Wang, Libin Zhang, Wei Huang
Summary: This study investigated the individual and combined toxicities of cadmium (Cd) and microplastics in Apostichopus japonicus and their effects on growth, Cd tissue accumulation, digestive enzymes, and gut microbes. Exposure to high concentrations of Cd and microplastics led to decreased body weight gain and specific growth rate. Cd content increased in the respiratory tree, digestive tract, and body wall. Digestive enzymes, such as amylase, lipase, and trypsin, decreased in the group exposed to high concentrations of Cd/microplastics. Firmicutes, especially Lactobacillales, were significantly reduced in multiple treatment groups. Cd had the greatest negative impact, but the presence of microplastics increased its toxicity.
MARINE ENVIRONMENTAL RESEARCH
(2023)
Article
Fisheries
Xiaoshang Ru, Qiming Feng, Shuangyan Zhang, Shilin Liu, Libin Zhang, Hongsheng Yang
Summary: In this study, an eco-friendly method for rearing sea cucumber larvae was developed by providing a suitable living environment in open-air outdoor ponds using a cage system. The optimal stocking density and substrate were identified to improve survival and settlement of A. japonicus larvae.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Jin-Liang Wei, Jing-Jing Cong, Zhi-Hui Sun, Jian Song, Chong Zhao, Ya-Qing Chang
Summary: Sex-specific markers were identified in sea cucumbers using a 2b-RAD-seq method, leading to the development of an efficient genetic sex identification method. This study sheds light on the sex determination mechanism in sea cucumbers and will enhance genetic and sex control breeding efficiency in this species.
Article
Fisheries
Wenyang Ma, Ya Li, Yiran Yang, Qingxi Han, Weiwei Zhang
Summary: The pacifastin gene Ajpacifastin-like was cloned and characterized in Apostichopus japonicus, showing ubiquitous expression in all tissues with the highest expression in muscle. The protein encoded by Ajpacifastin-like acts as an immunomodulatory factor via phenoloxidase to mediate phagocytosis by coelomocytes in pathogen-challenged A. japonicus.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Environmental Sciences
Zihe Zhao, Yushi Yu, Huiyan Wang, Xiyuan Huang, Peng Ding, Yingzhuo Sun, Jun Ding, Yaqing Chang, Chong Zhao
Summary: The study found that sea cucumbers exhibit different behavioral and physiological responses to the circadian rhythm. These findings suggest that aqua-farmers should consider carrying out seeding at night and providing diets before the peak of sea cucumber feeding at night. The study also revealed that cortisol concentration is higher at night, indicating that sea cucumbers may be more stressed during this time. However, 5-HT and melatonin are not significantly affected by the circadian rhythm. Overall, this study provides valuable insights into the behavior and physiology of sea cucumbers and their aquaculture.
MARINE ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Juanwen Yu, Yuichi Sakai, Sayaka Mino, Tomoo Sawabe
Summary: This study assessed the changes in microbial community during the larval development of sea cucumber using a laboratory rearing system. It was found that the microbial community structure was related to the developmental stage, with significant alterations detected in the late auricularia stage.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Evolutionary Biology
Boyang Hu, Yuan Tian, Qi Li, Shikai Liu
Summary: This study investigated the genomic signatures of long-term selective breeding in Pacific oysters, revealing the impact of artificial selection on genetic diversity and genomic features. Population structure analysis and identification of selective sweeps highlighted the genetic differences between fast-growing oyster strains and wild populations. The findings provide valuable insights for understanding the genetic basis of growth regulation in Pacific oysters and will be beneficial for future genome-assisted breeding programs.
EVOLUTIONARY APPLICATIONS
(2022)
Article
Fisheries
Ben Yang, Shangyu Zhai, Fuqiang Zhang, Hebing Wang, Liting Ren, Yongjing Li, Qi Li, Shikai Liu
Summary: This study conducted a genome wide association study in Pacific oysters to identify genetic loci underlying resistance to bacterial Vibrios. It revealed a polygenic architecture of resistance traits and identified 18 SNP loci and three QTL underlying the trait of resistance to Vibrio. In total, 178 genes involved in various processes of immune response were identified, providing valuable genetic resources for genomic selection breeding of Vibrio-resistant strains in the C. gigas.
Article
Biotechnology & Applied Microbiology
Chao Tan, Chenyu Shi, Yin Li, Wen Teng, Yongjing Li, Huiru Fu, Liting Ren, Hong Yu, Qi Li, Shikai Liu
Summary: This study investigated the role of DNA methylation in growth regulation of the Pacific oyster through analyzing the DNA methylome of two selectively bred C. gigas strains. The results revealed a widespread distribution of DNA methylation across the genome, with higher levels observed within genic regions. Functional annotation analysis identified genes enriched in various biological processes related to growth. Additionally, a positive correlation between gene expression and DNA methylation within gene-body regions was found. Integrative analysis of methylome and transcriptome profiles identified integrin beta-6 as a key regulator of the PI3K/Akt signaling pathway in growth-related processes.
MARINE BIOTECHNOLOGY
(2022)
Article
Zoology
Supannee Somrup, Akkarasiri Sangsawang, Nichanun McMillan, Supanida Winitchai, Jitti Inthoncharoen, Shikai Liu, Narongrit Muangmai
Summary: A new species of the genus Pinctada, Pinctada phuketensis sp. nov., is described from samples collected in Thailand. It is distinguished from other species by morphological and molecular data. Phylogenetic analysis supports its status as a distinct species closely related to Pinctada albina and Pinctada nigra.
Article
Genetics & Heredity
Billy Moore, Marcela Herrera, Emma Gairin, Chengze Li, Saori Miura, Jeffrey Jolly, Manon Mercader, Michael Izumiyama, Erina Kawai, Timothy Ravasi, Vincent Laudet, Taewoo Ryu
Summary: Anemonefish, especially the yellowtail clownfish Amphiprion clarkii, has unique characteristics that make it an ideal model organism for studying genetic, ecological, evolutionary, and developmental traits. The publication of a high-quality genome assembly of A. clarkii, along with its unique traits, provides valuable insights into its evolutionary history, behavioral strategies, fish-anemone symbiosis, and color pattern evolution. The transcriptomic analysis also revealed tissue-specific gene expression patterns, contributing to a better understanding of this species. Overall, this study highlights the importance of A. clarkii as a model organism for scientific research in various fields.
G3-GENES GENOMES GENETICS
(2023)
Article
Genetics & Heredity
Kuiqin Wang, Ben Yang, Qi Li, Shikai Liu
Summary: This study systematically compared ten genomic-prediction algorithms and validated them using genotype and phenotype data of five aquaculture species. The results showed that reproducing kernel Hilbert space and support-vector machine algorithms performed well in predicting most traits. The study also developed an R package to assist genomic selection breeding in aquaculture species.
Article
Engineering, Marine
Yiming Hu, Qi Li, Chengxun Xu, Shikai Liu, Lingfeng Kong, Hong Yu
Summary: This study investigated the differences in temperature and salinity tolerance between C. nippona and C. gigas spat and determined the optimal environmental conditions for the aquaculture of C. nippona spat. The results showed that C. nippona had lower survival rates than C. gigas under temperature changes and salinity decreases, but higher survival rates with increasing salinity. The findings suggest that particular attention should be paid to the inferior adaptability of C. nippona when farming outside their natural habitats.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Article
Materials Science, Paper & Wood
Bencheng Zhao, Chengze Li, Yongfang Chen, Qiong Tian, Yilmaz Yurekli, Fengxian Qiu, Tao Zhang
Summary: Wearable materials have attracted much attention for their versatile compatibility and utility in addressing the harsh living space caused by extreme temperatures and electromagnetic wave floods. In this study, a Janus MXene/cellulose/ZnO nanorods membrane was fabricated for accurate personalized thermal management and electromagnetic interference shielding. The obtained membrane exhibited great optical properties and radiative thermal management performances, with remarkable temperature differences achieved on both sides. Furthermore, the membrane showed excellent hydrophobicity and stain resistance, along with active Joule heating and superior electromagnetic interference shielding characteristics.
Article
Engineering, Electrical & Electronic
Yiwei Zhang, Chengze Li, Haiyang Wan, Qingxin Shi, Wenxia Liu, Yanhui Xu
Summary: In this paper, a collaborative stochastic expansion planning model of cyber-physical system (CPS) with resilience constraints is proposed, which can reduce coupling risk and enhance resilience under extreme scenarios. The model optimizes the siting of transmission lines, communication fibres, and service routing to minimize investment cost. Resilience constraints analyze the impact of cyber failures on dispatch strategy and limit load shedding in stochastic scenarios. The model is solved using the progressive hedging algorithm and case studies show its superiority over traditional independent planning models in limiting load loss and enhancing resilience under extreme scenarios.
IET GENERATION TRANSMISSION & DISTRIBUTION
(2023)
Article
Psychology, Multidisciplinary
Qisheng Zhan, Su Wang, Chengze Li, Mengri Li, Dan Liu, Wei Peng, Fanglin Song, Tongxiao Shi, Yonghui Li
Summary: This study revised and validated the Prosocialness Scale for Adults (PSA) for Chinese college students, providing a measurement tool for prosocial behavior among this population.
Article
Biochemistry & Molecular Biology
Yunze Liu, Haocheng Zheng, Anna Meilin Gu, Yuan Li, Tieshan Wang, Chengze Li, Yixiao Gu, Jie Lin, Xia Ding
Summary: Through the TCGA and GEO databases, we identified 173 metabolism-related genes (MRGs) associated with M2 macrophage infiltration in gastric cancer. Twelve MRGs were adopted as a prognostic signature and used to develop a risk model. Patients in the high-risk group had poorer survival outcomes and were less sensitive to certain drugs. Risk scores were correlated with immune cell infiltration, including CD8+ T cells and M2 macrophages. Single-cell sequencing analysis revealed differences in the expression and distribution of the 12 signature genes in the tumor microenvironment. In vitro experiments confirmed that Sorcin-knockdown gastric cancer cells suppressed M2 polarization of macrophages, inhibiting the proliferation and migration of gastric cancer cells. These findings provide a valuable prognostic signature for evaluating clinical outcomes, treatment options, and potential targets for gastric cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Computer Science, Software Engineering
Yinghua Liu, Chengze Li, Xueting Liu, Huisi Wu, Zhenkun Wen
Summary: In this work, a deep learning-based cartoon remastering application is proposed to improve the presentation of old cartoon animations by integrating noise removal, super-resolution, and color enhancement.
Article
Computer Science, Artificial Intelligence
Chenshu Xu, Xuemiao Xu, Nanxuan Zhao, Weiwei Cai, Huaidong Zhang, Chengze Li, Xueting Liu
Summary: Using a sequence of discrete still images to tell a story or introduce a process has become a tradition in the field of digital visual media. Comic, as a representative form, has gone digital and requires a different approach due to its unique characteristics. In this paper, the authors propose P-2 Comic, a model that takes page sequences of comics as input and uses panel-page understanding to classify the genre of the comic. Experimental results show that their approach outperforms existing methods and is applicable in multi-modal scenarios.
IEEE TRANSACTIONS ON IMAGE PROCESSING
(2023)
Proceedings Paper
Construction & Building Technology
Yingjie Tian, Fan Li, Yi Wu, Haoran Chen, Chengze Li, Xiu Yang
Summary: This study proposes a topology identification method for medium voltage distribution network based on Copula entropy. It aims to solve the problems of frequent changes in network topology and difficult identification. The method effectively measures the dependency of global variables without linear and Gaussian assumptions, and demonstrates its effectiveness and advantages through simulation.
2022 12TH INTERNATIONAL CONFERENCE ON POWER AND ENERGY SYSTEMS, ICPES
(2022)
Article
Fisheries
Fengping Li, Mingfu Fan, Shunshun Wang, Zhifeng Gu, Aimin Wang, Chunsheng Liu, Yi Yang, Shikai Liu
Summary: The complete mitochondrial DNA of the Gryphaeidae family, represented by Hyotissa hyotis, was sequenced for the first time and compared with other available oyster mitochondrial DNA. The study revealed significant differences in gene order and identified new features, such as the duplication of trnW. Phylogenetic analysis supported the close relationship between Gryphaeidae and Ostreidae. This study provides important insights into the mitochondrial evolution of oysters.
