Non-Linear Concentration-Response Relationships between Ambient Ozone and Daily Mortality

Background Ambient ozone (O-3) concentration has been reported to be significantly associated with mortality. However, linearity of the relationships and the presence of a threshold has been controversial. Objectives The aim of the present study was to examine the concentration-response relationship and threshold of the association between ambient O-3 concentration and non-accidental mortality in 13 Japanese and Korean cities from 2000 to 2009. Methods We selected Japanese and Korean cities which have population of over 1 million. We constructed Poisson regression models adjusting daily mean temperature, daily mean PM10, humidity, time trend, season, year, day of the week, holidays and yearly population. The association between O-3 concentration and mortality was examined using linear, spline and linear-threshold models. The thresholds were estimated for each city, by constructing linear-threshold models. We also examined the city-combined association using a generalized additive mixed model. Results The mean O-3 concentration did not differ greatly between Korea and Japan, which were 26.2 ppb and 24.2 ppb, respectively. Seven out of 13 cities showed better fits for the spline model compared with the linear model, supporting a non-linear relationships between O-3 concentration and mortality. All of the 7 cities showed J or U shaped associations suggesting the existence of thresholds. The range of city-specific thresholds was from 11 to 34 ppb. The city-combined analysis also showed a non-linear association with a threshold around 30-40 ppb. Conclusion We have observed non-linear concentration-response relationship with thresholds between daily mean ambient O-3 concentration and daily number of non-accidental death in Japanese and Korean cities.