Review
Biochemistry & Molecular Biology
Francesca Camponeschi, Mario Piccioli, Lucia Banci
Summary: Despite limited knowledge about the mechanism of mitoNEET protein, its link to cancer suggests that targeting mitoNEET and its Fe-S clusters could inhibit cancer cell proliferation. Spectroscopic techniques, such as NMR, have been used to study mitoNEET and understand the differences in cluster stability and reactivity, as well as its cellular function. NMR assignment and characterization of the first coordination sphere can aid in drug design to interfere with cellular processes or participate in redox reactions or protein-protein recognition mechanisms.
Article
Multidisciplinary Sciences
Ping Li, Amber L. Hendricks, Yong Wang, Rhiza Lyne E. Villones, Karin Lindkvist-Petersson, Gabriele Meloni, J. A. Cowan, Kaituo Wang, Pontus Gourdon
Summary: This study utilizes cryo-EM to reveal the structure of Atm1 transporter and proposes its molecular mechanism in the maturation of iron-sulfur proteins. These findings are of significant importance for understanding the transport mechanism of eukaryotic ABCB7-type proteins.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Sven-A Freibert, Michal T. Boniecki, Claudia Stuempfig, Vinzent Schulz, Nils Krapoth, Dennis R. Winge, Ulrich Muehlenhoff, Oliver Stehling, Miroslaw Cygler, Roland Lill
Summary: The conserved N-terminal Tyr35 of ISCU2 plays a crucial mechanistic role in de novo mitochondrial [2Fe-2S] cluster synthesis, with ionic forces replacing Tyr-Tyr interactions during dimerization-induced [2Fe-2S] cluster formation. Hydrophobic interaction of two conserved N-terminal tyrosines induces ISCU2 dimerization and concomitant [2Fe-2S] cluster synthesis.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Ke Zuo, Henri-Baptiste Marjault, Kara L. Bren, Giulia Rossetti, Rachel Nechushtai, Paolo Carloni
Summary: NEET proteins are a unique class of [2Fe-2S] proteins, existing in two redox states. Studies show that the reduced state of human NEET proteins is kinetically inert, contrasting with the reactivity of the oxidized state.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Zechariah Thompson, Insiya Fidai, Christine Wachnowsky, Amber L. Hendricks, J. A. Cowan
Summary: This study reveals the potential role of SyNfu as the principal scaffold protein in iron-sulfur cluster biosynthesis in Synechocystis. SyNfu can form a [2Fe-2S] dimeric protein complex and transfer clusters to various target proteins.
Article
Biochemistry & Molecular Biology
Kady M. Oakley, Ryan L. Lehane, Ziyi Zhao, Eunsuk Kim
Summary: In this study, a biomimetic [4Fe-4S] complex was reported to react with oxygen in the presence of thiolate and convert to a [2Fe-2S] analogue. The cluster conversion could also be achieved by using disulfide as an oxidant. Oxygen was found to facilitate the [4Fe-4S] to [2Fe-2S] cluster conversion at a faster rate than disulfide, although disulfide reaction produced higher yields of the [2Fe-2S] analogue.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Vinzent Schulz, Sven-A Freibert, Linda Boss, Ulrich Muehlenhoff, Oliver Stehling, Roland Lill
Summary: This review focuses on the structure, function and specificity of mitochondrial [2Fe-2S] FDXs and their important roles in various biological processes. Mitochondrial FDXs play crucial functions in steroid transformations, Fe/S protein biogenesis, and cofactor biosynthesis through electron transfer.
Article
Biochemistry & Molecular Biology
Brianne E. Lewis, Courtney J. Campbell, Andria Rodrigues, Lindsey Thompson, Ashutosh K. Pandey, Sarah N. Gallagher, Debkumar Pain, Andrew Dancis, Timothy L. Stemmler
Summary: This article investigates the role of the C-terminal region of the Isu1 protein in mitochondrial [2Fe-2S] cluster assembly and ISCU Myopathy in yeast. They find that the Isu1 C-terminus is crucial for Fe-S cluster assembly but also has independent functions beyond protein iron binding.
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Ola Karmi, Henri-Baptiste Marjault, Fang Bai, Susmita Roy, Yang-Sung Sohn, Merav Darash Yahana, Faruck Morcos, Konstantinos Ioannidis, Yaakov Nahmias, Patricia A. Jennings, Ron Mittler, Jose N. Onuchic, Rachel Nechushtai
Summary: The NEET protein family plays a crucial role in regulating mitochondrial labile iron and reactive oxygen species homeostasis. MiNT interacts with VDAC1 to transfer [2Fe-2S] clusters from mitochondria to the cytosol, impacting mitochondrial structure and function in breast cancer cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Michele Invernici, Giulia Selvolini, Jose Malanho Silva, Giovanna Marrazza, Simone Ciofi-Baffoni, Mario Piccioli
Summary: This study presents the effects of different iron-sulfide-glutathione ratios on the speciation of iron-sulfur cluster glutathione complexes. An excess of sulfide promotes the formation of a tetranuclear complex, while an excess of iron ions favors the formation of a dinuclear complex. These two complexes establish an interconversion equilibrium.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
R. Sophia Weerth, Amy E. Medlock, Harry A. Dailey
Summary: The article explores the distribution and function of [2Fe-2S] clusters in ferrochelatases involved in heme synthesis, revealing that while these clusters are rare within the bacterial ferrochelatase family, they are prevalent in certain phyla and correlate with microbial aerobic metabolism. Analysis also suggests that Firmicute ferrochelatases are the most ancient and preceded the Alphaproteobacterial precursor to eukaryotic mitochondria, shedding light on the evolution of these clusters.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sambuddha Sen, Amber L. Hendricks, James A. Cowan
Summary: Mitochondrial BOLA1 forms a [2Fe-2S] cluster-bridged heterodimeric complex with mitochondrial monothiol glutaredoxin GLRX5, with unclear function. This study explores the cluster exchange reactivity of the BOLA1-GLRX5 complex, demonstrating the ability to form the complex from certain donors but not others. The holo [2Fe-2S] BOLA1-GLRX5 complex is incapable of donating clusters to apo protein acceptors, providing evidence for a non-trafficking role. Additionally, the study compares the stability and cluster exchange reactivity of the holo-heterodimer and holo BOLA1 homodimer.
Article
Biochemistry & Molecular Biology
Kouhei Kunichika, Ryosuke Nakamura, Takashi Fujishiro, Yasuhiro Takahashi
Summary: The study determined the crystal structure of IscU from Methanothrix thermoacetophila, revealing a dimer structure with two [2Fe-2S] clusters in a specific arrangement. It was confirmed that two adjacent [2Fe-2S] clusters can be converted to a [4Fe-4S] cluster via reductive coupling in solution, with His106 identified to play a functional role in this process.
Article
Chemistry, Inorganic & Nuclear
Suman Dhara, Sanjib Panda, Goutam Kumar Lahiri
Summary: The facile reduction of 2,2'-dithiobisbenzothiazole through metal-to-ligand charge transfer or internal reducing equivalent is demonstrated, resulting in various binding modes of thiolates in a series of mononuclear and dinuclear ruthenium complexes. The electron transfer processes in the dinuclear complex resemble that of a [2Fe-2S] cluster.
DALTON TRANSACTIONS
(2021)
Article
Chemistry, Multidisciplinary
Alejandro Villalta, Batoul Srour, Audrey Lartigue, Martin Clemancey, Deborah Byrne, Florence Chaspoul, Antoine Loquet, Bruno Guigliarelli, Genevieve Blondin, Chantal Abergel, Benedicte Burlat
Summary: Scientists have discovered a protein in the giant virus mimivirus that is rich in the amino acids glycine and cysteine. This protein, called GciS, is one of the most abundant proteins in the viral particles and is likely essential for infection. It has unique binding properties with iron and inorganic sulfide.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Biochemistry & Molecular Biology
Ron Mittler, Merav Darash-Yahana, Yang Sung Sohn, Fang Bai, Luhua Song, Ioav Z. Cabantchik, Patricia A. Jennings, Jose N. Onuchic, Rachel Nechushtai
ANTIOXIDANTS & REDOX SIGNALING
(2019)
Article
Psychology, Educational
Ezgi Ayturk, Heining Cham, Patricia A. Jennings, Joshua L. Brown
EDUCATIONAL AND PSYCHOLOGICAL MEASUREMENT
(2020)
Article
Multidisciplinary Sciences
Colin H. Lipper, Jason T. Stofleth, Fang Bai, Yang-Sung Sohn, Susmita Roy, Ron Mittler, Rachel Nechushtai, Jose N. Onuchic, Patricia A. Jennings
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biochemistry & Molecular Biology
Dominique T. Capraro, David J. Burban, Patricia A. Jennings
JOURNAL OF MOLECULAR BIOLOGY
(2020)
Article
Mathematics, Interdisciplinary Applications
Ezgi Ayturk, Heining Cham, Patricia A. Jennings, Joshua L. Brown
Summary: The study found that LMS-cat is excellent at estimating structural parameters but may produce highly biased measurement parameters. The performance of LMS-cat varies under different conditions, as revealed by the simulation study.
STRUCTURAL EQUATION MODELING-A MULTIDISCIPLINARY JOURNAL
(2021)
Article
Physiology
Cynthia S. Indralingam, Alma K. Gutierrez-Gonzalez, Scott C. Johns, Tzuhan Tsui, Daniel T. Cannon, Mark M. Fuster, Timothy D. Bigby, Patricia A. Jennings, Ellen C. Breen
Summary: Pulmonary arterial hypertension (PAH) is a severe disease characterized by pulmonary artery remodeling, elevated right ventricular pressure, and cardiac failure. Pulmonary endothelial cells play a crucial role in PAH by sensing danger or damage and triggering cell proliferation through alarmin cytokines. This study found that IL-33/ST2 signaling is essential for the proliferative response of pulmonary artery endothelial cells in PAH.
PHYSIOLOGICAL REPORTS
(2022)
Article
Psychology, Educational
Polina P. Mischenko, Pamela Nicholas-Hoff, Deborah L. Schussler, Jessica Iwu, Patricia A. Jennings
Summary: This study provides an overarching analysis on the barriers and facilitators that implementers experienced in delivering a mindfulness-based social-emotional learning program. It emphasizes the importance of relational trust between implementers and actors within various ecological levels. The study applies a framework of relational trust to understand the relationships that influence the effectiveness of program delivery.
PSYCHOLOGY IN THE SCHOOLS
(2022)
Editorial Material
Psychology, Clinical
Patricia A. Jennings
Summary: There is a growing interest in studying the impacts of mindfulness-based programs in PreK-12 school settings, but the research lacks rigor and the results have been mixed. Baelen et al. present a conceptual framework for implementing school-based mindfulness programs, which will contribute to building a more solid evidence base. It is important to simultaneously focus on implementation practice to bridge the gap between knowledge and practice.
Article
Education & Educational Research
Holly E. Brophy-Herb, Ahnalee Brincks, Jody L. Cook, Ann Stacks, Claire D. Vallotton, Cynthia Frosch, Russell Carson, Rebecca Wheeler, Haiden A. Perkins, Patricia A. Jennings
Summary: This study examined the perceptions of stress and exhaustion among infant/toddler teachers. The findings revealed that workload and personal life stressors were associated with stress and exhaustion. Teachers used fewer than two coping strategies per day, and reframing was the only strategy negatively associated with stress and emotional exhaustion. The study highlights the need for professional development supports and workload reduction for infant/toddler teachers.
EARLY EDUCATION AND DEVELOPMENT
(2023)
Article
Education & Educational Research
Holly E. Brophy-Herb, Ann M. Stacks, Cynthia Frosch, Ahnalee M. Brincks, Jody L. Cook, Claire D. Vallotton, Haiden A. Perkins, Loria E. Kim, Russell Carson, Maria Muzik, Katherine Rosenblum, Patricia A. Jennings
Summary: The study focused on a relationship-based professional development intervention for infant and toddler teachers to enhance their use of mindfulness-based strategies for coping. While the intervention did not have an impact on stress and exhaustion trajectories, teachers in the intervention group reported increased use of mindfulness-based coping strategies over time.
EARLY CHILDHOOD EDUCATION JOURNAL
(2022)
Review
Psychology, Clinical
Patricia A. Jennings, Helen H. Min
Summary: Teachers' social and emotional competence and well-being are crucial for creating and maintaining supportive learning environments. However, teachers often lack the necessary support to provide trauma-sensitive care to students who have experienced adversity. This review explores the concept of empathy-based stress and proposes that shifting towards compassionate responding can help teachers effectively meet students' needs while protecting their own well-being.
Article
Psychology, Clinical
Sebrina L. Doyle L. Fosco, Deborah L. Schussler, Patricia A. Jennings
Summary: This research investigated how school and district leaders perceive and respond to an adapted version of the CARE program. The study found that acceptability of the program was inconsistent among educational leaders, with those with prior mindfulness experience benefiting the most. The format of the program also influenced acceptability, with many leaders finding it too long. However, all leaders reported using CARE practices and saw improvements in intrapersonal and interpersonal aspects.
Article
Education & Educational Research
Vanessa Rodriguez, S. Lynneth Solis, Bryan Mascio, Kathleen Kiely Gouley, Patricia A. Jennings, Laurie M. Brotman
EARLY EDUCATION AND DEVELOPMENT
(2020)
Article
Psychology, Educational
Damira Rasheed, Joshua Brown, Sebrina L. Doyle, Patricia A. Jennings
Article
Chemistry, Multidisciplinary
Anat Iosub-Amir, Fang Bai, Yang-Sung Sohn, Luhua Song, Sagi Tamir, Henri-Baptiste Marjault, Guy Mayer, Ola Karmi, Patricia A. Jennings, Ron Mittler, Jose N. Onuchic, Assaf Friedler, Rachel Nechushtai