Review
Biochemistry & Molecular Biology
Eerappa Rajakumara, Dubey Saniya, Priyanka Bajaj, Rajanna Rajeshwari, Jyotsnendu Giri, Mehdi D. D. Davari
Summary: Cytochrome P450s are important enzymes for the oxidative transformation of organic substrates. Factors such as protein structure, substrate binding, and redox potential modulation play a crucial role in determining the catalytic properties and selectivity of P450s. Understanding these factors is increasingly important in the fields of synthetic biology and biotechnology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Energy & Fuels
Qing Guo, Xiyuan Feng, Z. Conrad Zhang, Limin Huang
Summary: This paper focuses on recent achievements in establishing interfacial interactions between cocatalysts and semiconducting light absorbers to improve the efficiency of solar-light-driven redox reactions. Four kinds of interactions are summarized to clarify the accelerated photogenerated charge carrier kinetics, thereby enhancing the activity of these reactions. The extended applications of these interfacial interactions to other challenging reactions, such as N-2 fixation and organic synthesis, are also predicted.
Article
Chemistry, Multidisciplinary
Jiabao Wang, Yu Xu, Dandan Chen, Jiang Tao, Hongbo Wang, Wen Liu
Summary: In this study, a multifunctional cytochrome P450 enzyme, PyrE2, was discovered to catalyze the successive oxidation of an inert methyl group to produce a carboxyl product in pyrroindomycin biosynthesis. The time-course biotransformation revealed the presence of intermediates and a distributive mechanism for each oxidation step. Bioinformatics analysis showed that pyrE2 homologs are common in gene clusters related to spirotetronates, suggesting the generality and diversity of P450-catalyzed oxygenation in biosynthetic pathways.
CHINESE JOURNAL OF CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Nemanja Rajcevic, Danka Bukvicki, Tanja Dodos, Petar D. Marin
Summary: Plant-based natural products have been used as a source for therapeutics throughout history, with traditional medicine relying on a combination of plants to amplify the therapeutic potential. Understanding the synergistic effect of compounds is crucial in designing effective therapeutic agents.
Article
Pharmacology & Pharmacy
Zeyad Alehaideb, Mohamed Sheriffdeen, Francis C. P. Law
Summary: The study found that pre-treatment of volunteers with four ARFP extracts significantly inhibited caffeine metabolism, with increases in AUC(0-inf) ratio linearly related to the doses of furanocoumarins in the extracts. In vitro incubation studies showed that individual furanocoumarin bioactive compounds were potent inhibitors of caffeine-N-demethylation. CYP1A2 inactivation by these compounds was concentration- and time-dependent, involving irreversible inhibition mechanism.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Yohei Iizaka, David H. Sherman, Yojiro Anzai
Summary: Cytochrome P450 enzymes play a significant role in actinomycetes by catalyzing diverse oxidative reactions to produce a variety of compounds such as alcohols, ketones, aldehydes, and carboxylic acids. Understanding the enzymatic properties and reaction mechanisms of these enzymes can facilitate their utilization in biotechnological applications for creating high-value compounds.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Anand Mohan, Anupam Roy, Krishnabrunda Duggirala, Loren Klein
Summary: The problem of oxidative deterioration in meat and meat products is economically significant, causing loss of nutritional composition and product quality. This article emphasizes the importance of understanding oxidative processes and factors that influence protein degradation in muscle foods for the meat industry.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biology
Masaru K. Hojo
Summary: In this review, the author discusses how chemical communication based on condition-dependent signaling and recognition plasticity regulates the payoff in mutualistic associations. The selfishness of participants explains signaling and communication, contributing to the stability of these mutualisms. Future research on ant collective behavior, genetic and neural basis of cooperation, and a deeper understanding of the costs and benefits of these interactions will help uncover the origin and maintenance of ant mutualistic associations.
CURRENT OPINION IN INSECT SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Sharmila Samaroo, Charley Hengesbach, Chase Bruggeman, Nunzio Giorgio G. Carducci, Lincoln Mtemeri, Richard J. J. Staples, Thomas Guarr, David P. P. Hickey
Summary: This article investigates the correlation between the solubility of N-substituted pyridinium salts in a non-aqueous solvent and the C-H...p interactions. It finds a direct correlation between the two, highlighting the significant impact of minimal structural changes on pyridinium solubility.
Review
Biochemistry & Molecular Biology
Anna S. Alekseeva, Ivan A. Boldyrev
Summary: Secreted phospholipases A2 (sPLA2s) play important roles in various diseases, with their expression increasing significantly. Despite numerous proposed molecules as inhibitors, none have successfully passed clinical trials. The failures have led to in-depth studies on alternative ways to control sPLA2 activity. This review summarizes evidence and proposes new types of sPLA2 inhibitors.
Article
Biochemistry & Molecular Biology
Bikash Dangi, Nadezhda Y. Davydova, Marc A. Maldonado, Deepak Ahire, Bhagwat Prasad, Dmitri R. Davydov
Summary: This study investigated the relationship between drug metabolism routes and the composition of the P450 ensemble in human liver microsomes. The results showed that CYP2B6 and CYP3A5 play a crucial role in C152 metabolism. Analysis using PCA revealed significant differences between HLM samples, highlighting the pivotal role of P450-P450 interactions in determining drug metabolism routes.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Thirakorn Mokkawes, Tamar De Visser, Yuanxin Cao, Sam P. De Visser
Summary: In this study, a computational analysis was performed to investigate the binding and activation of different P450 isozymes with melatonin. The differences in product distributions were found to be primarily due to variations in the size and shape of the substrate binding pocket. P450 2C19 orients the substrate with its methoxy group towards the heme, resulting in O-demethylation products, while P450 1A1, 1A2, and 1B1 direct the substrate away from the heme and activate an alternative site, leading to aromatic hydroxylation instead.
Article
Multidisciplinary Sciences
Chuanwu Xia, Baoying Lou, Zhuji Fu, Al-Walid Mohsen, Anna L. Shen, Jerry Vockley, Jung-Ja P. Kim
Summary: ACAD9 is a dual function protein that catalyzes fatty acid beta-oxidation and is essential for mitochondrial respiratory complex I assembly. It interacts with ECSIT and NDUFAF1 to form the core mitochondrial CI assembly complex. Recent studies focus on the molecular mechanism of ACAD9/ECSIT/NDUFAF1 interactions.
Article
Thermodynamics
Dapeng Liu, Binod R. Giri, Milan Szori, Bela Viskolcz, Et-touhami Es-sebbar, Aamir Farooq
Summary: The study focused on measuring the absolute rate coefficients for the redox reaction between allyl radical (C3H5) and NOx, showing strong positive temperature dependence but no pressure dependence. This high-temperature measurement of allyl + NOx reactions provides valuable insights into the interaction of NOx with resonantly stabilized radicals and its effect on hydrocarbon fuels.
PROCEEDINGS OF THE COMBUSTION INSTITUTE
(2021)
Article
Chemistry, Physical
D. V. Pinakov, V. G. Makotchenko, G. I. Semushkina, G. N. Chekhova, I. P. Prosvirin, I. P. Asanov, Yu. V. Fedoseeva, A. A. Makarova, Yu. V. Shubin, A. V. Okotrub, L. G. Bulusheva
Summary: This study reports redox reactions occurring between acetonitrile and nitrogen oxide guests in galleries of fluorinated graphite, demonstrating the dissociation of N2O4 in fluorinated graphite matrices to form various nitrogen-containing species. The interaction of NO3 with acetonitrile yields HNO3 as a predominant guest in the synthesis products, leading to the removal of fluorine atoms weakly bonded to the graphite layers and partial defluorination of the matrices. The findings suggest the potential use of fluorinated graphite as a controllable test nanoreactor for various chemical reactions.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Review
Biochemistry & Molecular Biology
Zhong Li, Yuanyuan Jiang, F. Peter Guengerich, Li Ma, Shengying Li, Wei Zhang
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Jia Zhang, Yefei Wang, Baihui Chai, Jichao Wang, Lulu Li, Min Liu, Guang Zhao, Lishan Yao, Xiaolian Gao, Yifeng Yin, Jian Xu
ACS SYNTHETIC BIOLOGY
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Vandna Sharma, Brian Shing, Anjan Debnath, Larissa Podust
Article
Chemistry, Physical
Ying Ye, Lei Du, Xingwang Zhang, Sean A. Newmister, Morgan McCauley, Juan Alegre-Requena, Wei Zhang, Shuai Mu, Atsushi Minami, Amy E. Fraley, Maria L. Adrover-Castellano, Nolan A. Carney, Vikram V. Shende, Feifei Qi, Hideaki Oikawa, Hikaru Kato, Sachiko Tsukamoto, Robert S. Paton, Robert M. Williams, David H. Sherman, Shengying Li
Article
Chemistry, Medicinal
Zhong Li, Shuai Li, Lei Du, Xingwang Zhang, Yuanyuan Jiang, Wenhua Liu, Wei Zhang, Shengying Li
Summary: The positive regulatory roles of orf1 and bafG in bafilomycin biosynthesis were characterized, with their overexpression significantly increasing bafilomycin production. The genetically engineered S. lohii strain produced bafilomycin A(1) solely with high titer, holding potential for large-scale production for new drug development.
Article
Biotechnology & Applied Microbiology
Sheng Dong, Jingfei Chen, Xingwang Zhang, Fei Guo, Li Ma, Cai You, Xiao Wang, Wei Zhang, Xiaobo Wan, Shuang-Jiang Liu, Li-Shan Yao, Shengying Li, Lei Du, Yingang Feng
Summary: Selective oxidation of C-H bonds in alkylphenols is crucial for structural derivatization in pharmaceutical and biomanufacturing, as well as for biological degradation of toxic chemicals in environmental protection. A novel chemomimetic biocatalytic system using cytochrome P450 monooxygenase CreJ has been developed, showing excellent regio- and stereoselectivity in oxidizing various alkylphenol substrates. The results provide mechanistic insights into CreJ regio- and stereoselectivity, offering promising directions for further enzyme design and engineering efforts.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiangfei Song, Liaoyuan An, Mengting Wang, Jingfei Chen, Zhijun Liu, Lishan Yao
Summary: This study found that glycerol stabilizes protein folding, while betaine and taurine destabilize protein folding by affecting folding rates and instability. Betaine and taurine enhance quinary interactions between the protein and cellular environment, weakening electrostatic attraction in protein salt bridges, leading to protein destabilization in E. coli cells. These factors counteract the preferential exclusion mechanism used by osmolytes to stabilize proteins.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Cai You, Fengwei Li, Xingwang Zhang, Li Ma, Yu-Zhong Zhang, Wei Zhang, Shengying Li
Summary: Mycophenolic acid (MPA) is a fungal natural product and primary immunosuppressive drug used in organ transplantations and autoimmune diseases. The study of the MpaH' enzyme, which hydrolyzes MPA-CoA with high specificity in peroxisomes, reveals a new subfamily of alpha/beta hydrolase fold proteins with unique structural features.
Article
Chemistry, Multidisciplinary
Xiangfei Song, Mengting Wang, Xiaoxu Chen, Xueying Zhang, Ying Yang, Zhijun Liu, Lishan Yao
Summary: This study investigated how the cellular environment affects electrostatic interactions between protein charges using in-cell nuclear magnetic resonance spectroscopy. The results showed that the transfer free energy in cells can modulate protein electrostatic interactions, with a larger effect on the interactions in the unfolded state compared to the folded state. This work highlights the importance of direct in-cell studies for understanding protein interactions and function.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Xiaoyan Liu, Mingming Dong, Yating Yao, Yan Wang, Jiawei Mao, Lianghai Hu, Lishan Yao, Mingliang Ye
Summary: This study presents an antibody-free approach to identify phosphotyrosine peptides by utilizing protein tyrosine phosphatase (PTP). By combining two Ti4+-IMAC-based phosphopeptide enrichment steps with PTP-catalyzed dephosphorylation, a cost-effective alternative for tyrosine phosphoproteomics analysis is provided.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Tingting Liu, Han Wu, Xiaofan Du, Jinzhi Wang, Zheng Chen, Hao Wang, Jinran Sun, Jianjun Zhang, Jiaping Niu, Lishan Yao, Jingwen Zhao, Guanglei Cui
Summary: This study presents a hydrated eutectic electrolyte with a water-locked effect, which strengthens the O-H bond of water via weak hydrogen bonding, leading to improved rechargeability and stability of aqueous sodium batteries.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Multidisciplinary Sciences
Mengting Wang, Xiangfei Song, Jingfei Chen, Xiaoxu Chen, Xueying Zhang, Ying Yang, Zhijun Liu, Lishan Yao
Summary: Conformational dynamics of proteins play a crucial role in cellular functions. However, the influence of the intracellular environment on protein conformational dynamics is not well understood. In this study, the loop conformational dynamics of a model protein in Escherichia coli cells was investigated using nuclear magnetic resonance (NMR) spectroscopy. Weak interactions between the protein and surrounding macromolecules in cells hindered the rotational diffusion of the protein, allowing the detection of dynamics in the microsecond timescale using NMR spin relaxation method. The loop dynamics in the picosecond to microsecond range were confirmed using nanoparticle-assisted spin relaxation and residual dipolar coupling methods. Point mutation of the loop sequence perturbed the loop interactions with the intracellular environment. For the protein sequence with stronger interactions with surrounding macromolecules, the loop became more rigid in cells. In contrast, the effect of mutation on the loop dynamics in vitro was small. This study provides direct evidence that the intracellular environment can modify protein loop conformational dynamics through weak interactions.
Article
Chemistry, Analytical
Yefei Wang, Liaoyuan An, Ying Yang, Lishan Yao
ANALYTICAL CHEMISTRY
(2020)
Article
Biotechnology & Applied Microbiology
Fengwei Li, Li Ma, Xingwang Zhang, Jingfei Chen, Feifei Qi, Yinyue Huang, Zepeng Qu, Lishan Yao, Wei Zhang, Eung-Soo Kim, Shengying Li
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2020)