期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 136, 期 28, 页码 9874-9877出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja505122n
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资金
- RFBR [14-03-31839, 12-04-01435, 13-04-00680, 13-04-01176]
- MES RF [14.B25.31.0028]
Resolving the nanometer-scale structure of biomolecules in natural conditions still remains a challenging task. We report the first distance measurement in nucleic acid at physiological temperature using electron paramagnetic resonance (EPR). The model 10-mer DNA duplex has been labeled with reactive forms of triarylmethyl radicals and then immobilized on a sorbent in water solution and investigated by double quantum coherence EPR. We succeeded in development of optimal triarylmethyl-based labels, approach for site-directed spin labeling and efficient immobilization procedure that, working together, allowed us to measure as long distances as similar to 4.6 nm with high accuracy at 310 K (37 degrees C).
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