期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 136, 期 22, 页码 8131-8137出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja503864v
关键词
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资金
- Gottingen Graduate School for Neurosciences, Biophysics, and Molecular Biosciences (GGNB) [DFG GSC 226/2]
- Cluster of Excellence
- DFG Research Center Nanoscale Microscopy and Molecular Physiology of the Brain (CNMPB)
- International Research Training Group Metal Sites in Biomolecules [IRTG1422]
- Max Planck Society
A general and efficient single-step method was established for site-specific post-transcriptional labeling of RNA. Using Tb3+ as accelerating cofactor for deoxyribozymes, various labeled guanosines were site-specifically attached to 2'-OH groups of internal adenosines in in vitro transcribed RNA. The DNA-catalyzed 2',5'-phosphodiester bond formation proceeded efficiently with fluorescent, spin-labeled, biotinylated, or cross-linker-modified guanosine triphosphates. The sequence context of the labeling site was systematically analyzed by mutating the nucleotides flanking the targeted adenosine. Labeling of adenosines in a purine-rich environment showed the fastest reactions and highest yields. Overall, practically useful yields >70% were obtained for 13 out of 16 possible nucleotide (nt) combinations. Using this approach, we demonstrate preparative labeling under mild conditions for up to similar to 160-nt-long RNAs, including spliceosomal U6 small nuclear RNA and a cyclic-di-AMP binding riboswitch RNA.
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