期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 135, 期 51, 页码 19147-19153出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja4114193
关键词
-
资金
- National Science Foundation-Major Research Instrument Development Program [DBI-0821700]
- Department of Energy, Division of Chemical Sciences [BES DE-FG02-04ER15520]
- National Science Foundation [CHE-0541587]
Past experimental results and molecular dynamics simulations provide evidence that, under some conditions, electrospray ionization (ESI) of biomolecules produces ions that retain elements of solution phase structures. However, there is a dearth of information regarding the question raised by Breuker and McLafferty, for how long, under what conditions, and to what extent, can solution structure be retained without solvent? (Proc. Natl. Acad. Sci. U.S.A. 2008, 105, 18145). Here, we use cryogenic ion mobility-mass spectrometry to experimentally probe the structural evolution of the undecapeptide substance P (SP) during the final stages of ESI. The results reveal that anhydrous SP conformers originate from evaporation of cluster ions, specifically, [SP + 2H](2+) (H2O)(n) (n = 0 to similar to 50) and [SP + 3H](3+) (H2O) (n = 0 to similar to 30), and that major structural changes do not occur during the evaporative process. In the case of [SP + 3H](3+), the results demonstrate that a compact dehydrated conformer population can be kinetically trapped on the time scale of several milliseconds, even when an extended gas phase conformation is energetically favorable.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据