期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 135, 期 6, 页码 2037-2039出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja312032c
关键词
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资金
- U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-FG02-07ER15865, DE-AC02-06CH11357]
- U.S. Department of Energy (DOE) [DE-FG02-07ER15865] Funding Source: U.S. Department of Energy (DOE)
The Salmonella PmrA/PmrB two-component system uses an iron(III)-binding motif on the cell surface to sense the environmental or host ferric level and regulate PmrA-controlled gene expression. We replaced the iron(III)-binding motif with a lanthanide-binding peptide sequence that is known to selectively recognize trivalent lanthanide ions. The newly engineered two-component system (PmrA/PmrB) can effectively sense lanthanide ion and regulate gene expression in E. coli. This work not only provides the first known lanthanide-based sensing and response in live cells but also demonstrates that the PmrA/PmrB system is a suitable template for future synthetic biology efforts to construct bacteria that can sense and respond to other metal ions in remediation or sequestration.
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