Editorial Material
Biochemistry & Molecular Biology
F. Peter Guengerich
Summary: The paper introduces the concept of cytochrome b(5) as an auxiliary protein facilitating cytochrome P450 reactions, providing important insights for future research.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Hebatalla Mohamed, Amna Ghith, Stephen G. Bell
Summary: The cytochrome P450 superfamily of enzymes can catalyse oxidation reactions and their heme and active site environment can be studied using UV-visible absorbance spectroscopy. The binding of imidazole and pyridine-based ligands to different forms of bacterial cytochrome P450 enzymes was evaluated using this technique. The majority of ligands interacted with the heme as expected, but differences in heme environment were observed across enzyme/ligand combinations.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Xiangquan Qin, Yiping Jiang, Fuquan Yao, Jie Chen, Fanhui Kong, Panxia Zhao, Longyi Jin, Zhiqi Cong
Summary: In this study, artificial metalloenzymes (ArMs) were constructed by optimizing the structure of dual-functional small molecules (DFSMs) to enhance their binding affinity with P450 enzymes. This strategy successfully resulted in the formation of P450BM3-based peroxygenases with high catalytic activity and promiscuity. The use of editable organic cofactors as co-catalytic centers allows for a low load requirement and widens the substrate range for peroxygenase reactions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Inorganic & Nuclear
Lu-Lu Liu, Li-Xin Wang, Min Peng, Jing Xiang, Huan Yang, Shek-Man Yiu, Tai-Chu Lau
Summary: The reactivity of electrophilic OsVI and RuVI nitrides with amines has been reported before. In this study, a luminescent osmium(VI) nitrido complex was found to undergo ring nitrogenation of aromatic amines under visible light irradiation. The reaction proceeds through nucleophilic addition of the amine nitrogen to the excited state complex. The products were characterized using spectroscopic techniques and X-ray crystallography. The reaction mechanism involves electron transfer and proton transfer steps.
INORGANIC CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Chiemela S. S. Odoemelam, Elena Hunter, Zeeshan Ahmad, Caroline Lynn Kamerlin, Samuel White, Philippe B. B. Wilson
Summary: This study investigated the interactions of selected flavonoids with cytochrome P450 enzymes and found that isorhamnetin and pedalitin had the strongest binding energies. These flavonoids may serve as potential precursors for natural product-derived therapies.
CURRENT PHARMACEUTICAL DESIGN
(2022)
Article
Chemistry, Inorganic & Nuclear
Kyungmin Kim, Seongmin Oh, Donghyun Jeong, Yuri Lee, Dohyun Moon, Sunggi Lee, Jaeheung Cho
Summary: In this study, a series of cobalt(III)-peroxo complexes and cobalt(III)-hydroperoxo complexes were synthesized and characterized. The results showed that their structures and properties were influenced by the electronic effects of the ligands, providing important insights into the electronic nature-reactivity relationship of metal-oxygen species.
INORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Christopher S. Campomizzi, George E. Ghanatios, D. Fernando Estrada
Summary: Cytochromes P450 are versatile enzymes involved in endogenous and exogenous metabolism, undergoing structural changes related to function. This study demonstrates the utility of fluorine (19F)-NMR spectroscopy in monitoring structural changes in CYP121A1, revealing insights into its role in substrate recognition and mechanistic details of this essential enzyme from Mycobacterium tuberculosis.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Andre Fischer, Martin Smiesko
Summary: This study investigates an allosteric site in Cytochrome P450 enzymes, finding it to be well conserved in the CYP2 family with the exception of CYP2E1, and observing an enlargement of ligand tunnels in certain members that facilitate ligand translocation. The research contributes to understanding ligand access phenomena and aids in estimating the selectivity of metabolic transformations using computational techniques.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Bankala Krishnarjuna, Sang -Ghoul Im, Thirupathi Ravula, Joseph Marte, Richard J. Auchus, Ayyalusamy Ramamoorthy
Summary: This study reports the successful functional reconstitution of a membrane-bound redox complex in non-ionic inulin-based polymer nanodiscs. The nanodiscs have a higher phase-transition temperature and can align in an externally applied magnetic field. These findings suggest that non-ionic polymer nanodiscs can be used for studying the function and structures of various membrane proteins/complexes.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Inorganic & Nuclear
Williamson N. Oloo, Miklos Szavuly, Jozsef Kaizer, Lawrence Que
Summary: This study describes a substrate oxidation reaction catalyzed by [Fe-II(IndH)(CH3CN)(3)](ClO4)(2) with H2O2, involving a spectroscopically characterized intermediate capable of olefin epoxidation and alkane hydroxylation. The research identified a direct nucleophilic attack of the substrate carbonyl group by the peroxo species. Additionally, the study found that the high-valent iron-oxo oxidant derived from O-O bond cleavage of the peroxo intermediate contributes to the oxidative reactivities associated with nonheme diiron enzymes.
INORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Xinkun Ren, Bo M. Couture, Ningyu Liu, Manjinder S. Lall, Jeffrey T. Kohrt, Rudi Fasan
Summary: This study reports the development of an iron-based biocatalytic strategy for enantioselective alpha-C-H functionalization of pyrrolidines and other saturated N-heterocycles, using a carbene transfer reaction with diazoacetone. The method shows high yields, catalytic activity, and stereoselectivity, and is applicable to drug scaffolds synthesis.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Jing Xiang, Min Peng, Yi Pan, Li-Juan Luo, Shun-Cheung Cheng, Xin-Xin Jin, Shek-Man Yiu, Wai-Lun Man, Chi-Chiu Ko, Kai-Chung Lau, Tai-Chu Lau
Summary: The study presented the first example of N-dealkylation of various alkylamines by a luminescent osmium(VI) nitrido complex induced by visible light.
Article
Biochemistry & Molecular Biology
Tatsiana Sushko, Anton Kavaleuski, Irina Grabovec, Anna Kavaleuskaya, Daniil Vakhrameev, Sergey Bukhdruker, Egor Marin, Alexey Kuzikov, Rami Masamrekh, Victoria Shumyantseva, Kouhei Tsumoto, Valentin Borshchevskiy, Andrei Gilep, Natallia Strushkevich
Summary: Research finds that RubB efficiently transfers electrons from cognate reductases to support bacterial CYPs activity, mutations on RubB surface do not significantly decrease CYPs activity, suggesting interactions are transient and nonspecific.
BIOORGANIC CHEMISTRY
(2021)
Article
Chemistry, Inorganic & Nuclear
Ge Tian, Gangping Hao, Xiaohua Chen, Yongjun Liu
Summary: The study investigates the decarboxylation mechanism of Coproporphyrin III, revealing the crucial role of Tyr144 in the decarboxylation of P2 propionate and the difficulty of Lys148-mediated decarboxylation of P4, suggesting the possibility of rearrangement of the generated harderoheme III intermediate in the active site.
INORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Alexander Brinkmeier, Kristian E. Dalle, Lorenzo D'Amore, Roland A. Schulz, Sebastian Dechert, Serhiy Demeshko, Marcel Swart, Franc Meyer
Summary: The study revealed the strong binding of alkali metal ions to reactive cis-peroxo dicopper(II) intermediates, affecting the electronic and electrochemical properties of the core structure and leading to changes in charge transfer dynamics. This has significant implications for understanding oxidation processes in synthetic and natural environments.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biochemistry & Molecular Biology
Geoffrey A. Heinzl, Weiliang Huang, Elizabeth Robinson, Fengtian Xue, Pierre Moeenne-Loccoz, Angela Wilks
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Dante Avalos, Sinan Sabuncu, Kyle J. Mamounis, Victor L. Davidson, Pierre Moenne-Loccoz, Erik T. Yukl
Article
Chemistry, Multidisciplinary
Jesse B. Gordon, Avery C. Vilbert, Maxime A. Siegler, Kyle M. Lancaster, Pierre Moenne-Loccoz, David P. Goldberg
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Multidisciplinary
Melanie A. Ehudin, Leland B. Gee, Sinan Sabuncu, Augustin Braun, Pierre Moenne-Loccoz, Britt Hedman, Keith O. Hodgson, Edward I. Solomon, Kenneth D. Karlin
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Multidisciplinary
Jeffrey J. Liu, Maxime A. Siegler, Kenneth D. Karlin, Pierre Moenne-Loccoz
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2019)
Article
Chemistry, Inorganic & Nuclear
Alex M. Confer, Sinan Sabuncu, Maxime A. Siegler, Pierre Moenne-Loccoz, David P. Goldberg
INORGANIC CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Jesse B. Gordon, Avery C. Vilbert, Ida M. DiMucci, Samantha N. MacMillan, Kyle M. Lancaster, Pierre Moenne-Loccoz, David P. Goldberg
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Article
Chemistry, Multidisciplinary
Jorge L. Martinez, Sean A. Lutz, Daniel M. Beagan, Xinfeng Gao, Maren Pink, Chun-Hsing Chen, Veronica Carta, Pierre Moenne-Loccoz, Jeremy M. Smith
ACS CENTRAL SCIENCE
(2020)
Article
Chemistry, Multidisciplinary
Kelsey R. Miller, Saborni Biswas, Andrew Jasniewski, Alec H. Follmer, Ankita Biswas, Therese Albert, Sinan Sabuncu, Emile L. Bominaar, Michael P. Hendrich, Pierre Moenne-Loccoz, A. S. Borovik
Summary: The research focuses on designing artificial proteins to achieve active sites with entatic states by simulating a controlled environment. Utilizing biotin-streptavidin technology, artificial di-Fe cores containing Fe centers have been successfully assembled, revealing the process of Fe center separation regulation by the protein host.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Inorganic & Nuclear
Cayden X. Bullock, Cooper S. Jamieson, Pierre Moenne-Loccoz, Buck Taylor, Jordan A. M. Gonzalez, Ellie A. Draves, Louis Y. Kuo
Summary: A new class of dioxomolybdenum(VI) catalyst is used to achieve sulfide oxidation, with experiments and computational modeling supporting a hypothesized reaction mechanism.
INORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Aniruddha Dey, Jesse B. Gordon, Therese Albert, Sinan Sabuncu, Maxime A. Siegler, Samantha N. MacMillan, Kyle M. Lancaster, Pierre Moenne-Loccoz, David P. Goldberg
Summary: A new nonheme iron(II) complex was reported, which reacts with NO(g) to form a stable mononitrosyl complex. The mononitrosyl complex exhibits highly activated characteristics and can rapidly convert to N2O in the absence of an exogenous reductant.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemistry & Molecular Biology
Alecia T. Dent, Marley Brimberry, Therese Albert, William N. Lanzilotta, Pierre Moenne-Loccoz, Angela Wilks
Summary: Interactions between Y75H holo-HasAp and HasR inhibit the release of heme for signaling and uptake, as shown by spectroscopic characterization. The structural features and ligand field of Y75H holo-HasAp are consistent with intermediate values, supported by EPR spectrum and crystal structure analysis.
Article
Chemistry, Multidisciplinary
Jireh Joy D. Sacramento, Therese Albert, Maxime Siegler, Pierre Moenne-Loccoz, David P. Goldberg
Summary: A new ferrous corrole complex [Fe-II(ttppc)](-) has been shown to bind dioxygen to form Fe-O-2, exhibiting an O-16/18(2)-isotope sensitive n(O-O) stretch at 1128 cm(-1) and a single n(Fe-O-2) at 555 cm(-1). This complex can mediate the dioxygenation of an indole derivative and extract hydrogen atoms from activated O-H bonds.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Ravikumar Reddi, Kimberly Matulef, Erika Riederer, Pierre Moenne-Loccoz, Francis Valiyaveetil
Summary: Regulation of ion conduction through the pore of a K+ channel involves coordinated action of the activation gate and inactivation gate, with the W67F mutant of the KcsA channel showing reduced inactivation and enhanced activation rate. Structural studies provide insights into the allosteric pathway and highlight the importance of ion occupancy at the S2 site for channel inactivation.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Shiliang Tian, Ruixi Fan, Therese Albert, Rahul L. Khade, Huiguang Dai, Kevin A. Harnden, Parisa Hosseinzadeh, Jing Liu, Mark J. Nilges, Yong Zhang, Pierre Moenne-Loccoz, Yisong Guo, Yi Lu
Summary: This study successfully achieved stepwise nitrosylation of a mononuclear nonheme iron site in an engineered azurin under physiological conditions, controlled formation of {FeNO}(7) and {Fe(NO)(2)}(9) species by tuning the nitric oxide equivalents and reaction time. The potential formation of the elusive {FeNO}(8) species was inferred by EPR spectroscopy, while the conversion of {FeNO}(7) to {Fe(NO)(2)}(9) species was supported by various spectroscopic techniques.