Article
Cell Biology
Yoko K. Takada, Masaaki Fujita, Yoshikazu Takada
Summary: The study showed that inflammatory chemokines can allosterically activate platelet integrin αIIbβ3, triggering platelet aggregation, which may serve as a missing link between inflammation and thrombosis.
Article
Hematology
Aster E. Pijning, Mitchell T. Blyth, Michelle L. Coote, Freda Passam, Joyce Chiu, Philip J. Hogg
Summary: Research has shown that the αIIbβ3 integrin receptor is produced in various covalent forms with different cell surface distribution and function. The C490, C545 cysteine pair is conserved across all 18 integrin alpha subunits, suggesting that similar forms and functions of integrin are also conserved in cultured cells.
Review
Biochemistry & Molecular Biology
Jinmi Zou, Frauke Swieringa, Bas de Laat, Philip G. de Groot, Mark Roest, Johan W. M. Heemskerk
Summary: This paper reviews the functional reversibility of integrin alpha IIb beta 3 activation and transient aggregation and discusses the signaling pathways involved. The study finds that ADP P2Y(1) and P2Y(12) receptor signaling via phosphoinositide 3-kinases and Akt are the principal pathways linked to reversible integrin activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Ming-Ming Zhang, Ling-Ling Luo, Yu Liu, Gui-Jie Wang, Huan-Huan Zheng, Xu-Sheng Liu, Jia-Lin Wang
Summary: This study reveals the interaction between insect CIB1-like and the integrin alpha-cytoplasmic domain, which facilitates cell adhesion. This finding enhances our understanding of the molecular mechanism underlying integrin-mediated cellular immunity in insects.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2022)
Article
Cell Biology
Lina M. Gallego-Paez, William J. S. Edwards, Manasa Chanduri, Yanyu Guo, Thijs Koorman, Chieh-Yu Lee, Nina Grexa, Patrick Derksen, Jie Yan, Martin A. Schwartz, Jan Mauer, Benjamin Thomas Goult
Summary: This study identified a previously unannotated cassette exon, referred to as exon 17b, in the TLN1 gene. The presence of this exon alters the function of the TLN1 protein in the mechanosensitive adapter system and affects cell adhesion dynamics and motility. This novel exon is detectable in multiple healthy tissues and significantly enriched in certain cancer subtypes.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Daniel Gao, Caroline W. Sun, Angela B. Woodley, Jing-fei Dong
Summary: Clot retraction, which requires the functional platelet alpha(IIb)beta(3) integrin, is an important measure of platelet function and can be used to detect functional changes in platelets. Platelet counts and the surface density of the integrin alpha(IIb)beta(3) affect clot retraction.
Article
Multidisciplinary Sciences
Annemiek Dickhout, Bibian M. E. Tullemans, Johan W. M. Heemskerk, Victor L. J. L. Thijssen, Marijke J. E. Kuijpers, Rory R. Koenen
Summary: This study showed that gal-1 and CXCL4 colocalize on the platelet surface and co-activation of platelets by both can enhance aggregation. Their individual effects on platelets exhibit distinct and complementary activation profiles, with additive rather than synergistic effects.
Article
Urology & Nephrology
Veronika Binder, Barbara Chruscicka-Smaga, Brith Bergum, Stephane Jaisson, Philippe Gillery, Joar Sivertsen, Tor Hervig, Marta Kaminska, Ronak Tilvawala, Venkatesh V. Nemmara, Paul R. Thompson, Jan Potempa, Hans-Peter Marti, Piotr Mydel
Summary: The study explored carbamylation as a potential mechanistic link between uremia and platelet dysfunction in patients with ESKD, revealing that carbamylation inhibited platelet activation, adhesion, and aggregation.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2022)
Article
Chemistry, Multidisciplinary
Mingxi Li, Liang Wang, Dalin Tang, Gutian Zhao, Zhonghua Ni, Ning Gu, Fang Yang
Summary: Platelet membrane biomimetic nanobubbles (PNBs) with ideal echogenicity were fabricated using an adjustable pressure-induced shear stress method, enriching platelet membrane lipid rafts and proteins during high shear stress. The conformation of platelet integrin alpha(IIb)beta(3) was transformed into a shear stress-induced intermediate affinity state, enhancing PNBs' adhesion ability to vascular endothelial injury for potential targeted ultrasound diagnosis.
Article
Oncology
Sven Jacob, Florian Boesch, Markus B. Schoenberg, Elise Pretzsch, Christopher Lampert, Ren Haoyu, Bernhard W. Renz, Marlies Michl, Jorg Kumbrink, Thomas Kirchner, Jens Werner, Martin K. Angele, Jens Neumann
Summary: This study identified CIB1 as significantly overexpressed in CRC leading to liver metastases compared to M0 and PER. The findings suggest that CIB1 may play a crucial role in hematogenous spread to the liver but not for peritoneal carcinomatosis. Consequently, CIB1 seems to be a promising prognostic marker and a potential tool for future targeted therapies as well as early diagnostics and follow-up.
Article
Hematology
Ana Kasirer-Friede, Emilia Peuhu, Johanna Ivaska, Sanford J. Shattil
Summary: This study demonstrates the involvement of SHARPIN in regulating important functions of platelets, including inflammation and integrin adhesion. Platelets lacking SHARPIN showed enhanced spreading and adhesion on fibrinogen, but compromised thrombus growth on collagen. The loss of SHARPIN also resulted in reduced NF-kappa B activation and linear ubiquitination of protein substrates in platelets, and significantly reduced inflammation in mouse models.
Article
Biophysics
Dudu Tong, Nidhi Soley, Reza Kolasangiani, Martin A. Schwartz, Tamara C. Bidone
Summary: The platelet integrin aIIbb3 undergoes conformational transitions from inactive to active states during hemostasis. The properties and functions of intermediate conformations, which are between the well-characterized bent and extended states, are largely unexplored. In this study, the researchers evaluated the properties of intermediate conformations of integrin aIIbb3 and found that they stabilize adhesions. These findings provide insights into the mechanisms of aIIbb3 integrin adhesion stability.
BIOPHYSICAL JOURNAL
(2023)
Article
Hematology
Karen P. Fong, Kathleen S. Molnar, Nicholas Agard, Rustem Litvinov, Oleg Kim, James A. Wells, John W. Weisel, William F. DeGrado, Joel S. Bennett
Summary: Blood clot contraction is driven by traction forces generated by the platelet cytoskeleton and regulated by integrin alpha IIb beta 3. Proteolytically cleaved platelet proteins, particularly talin and vinculin, play crucial roles in clot contraction. Calpain-mediated cleavage of these proteins occurs independent of cytoskeletal-generated tensile forces, suggesting calpain's involvement in later stages of clot retraction through focal adhesion disassembly.
Review
Biochemistry & Molecular Biology
Marco Pedretti, Luca Bombardi, Carolina Conter, Filippo Favretto, Paola Dominici, Alessandra Astegno
Summary: Centrins are a family of small proteins containing EF hands found in all eukaryotes and often complexed with centrosome-related structures, displaying diverse cellular functions and large variance in Ca2+ sensing abilities compared to calmodulin (CaM). Current knowledge on centrins from biophysical and structural perspectives emphasizes centrin-target interactions, providing insight into their different functions and contribution to the complexity of Ca2+ signaling cascade. This understanding can help clarify the functional redundancy of centrins and centrin-binding proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Jung-Hae Shin, Hyuk-Woo Kwon, Muhammad Irfan, Man Hee Rhee, Dong-Ha Lee
Summary: The study showed that G-Rk1 has an inhibitory effect on human platelet aggregation, particularly more effective on collagen-stimulated platelet aggregation. It can also suppress the activity of various platelet signaling molecules, thus blocking platelet formation of thrombosis.
JOURNAL OF GINSENG RESEARCH
(2021)