期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 134, 期 1, 页码 247-255出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja205524x
关键词
-
资金
- National Science Foundation [CHE-0645793]
- Center for Scalable and Integrated NanoManufacturing (SINAM) [DMI-0327077]
- NSF IGERT [DGE-0654431]
- California NanoSystems Institute
- Alfred P. Sloan Foundation
- Swiss National Science Foundation [PBEZP2- 133211]
- Swiss National Science Foundation (SNF) [PBEZP2-133211] Funding Source: Swiss National Science Foundation (SNF)
- Directorate For Engineering [0751621] Funding Source: National Science Foundation
- Div Of Civil, Mechanical, & Manufact Inn [0751621] Funding Source: National Science Foundation
Understanding and controlling cell adhesion on engineered scaffolds is important in biomaterials and tissue engineering. In this report we used an electron-beam (e-beam) lithography technique to fabricate patterns of a cell adhesive integrin ligand combined with a growth factor. Specifically, micron-sized poly(ethylene glycol) (PEG) hydrogels with aminooxy- and styrene sulfonate-functional groups were fabricated. Cell adhesion moieties were introduced using a ketone-functionalized arginine-glycine-aspartic acid (RGD) peptide to modify the O-hydroxylamines by oxime bond formation. Basic fibroblast growth factor (bFGF) was immobilized by electrostatic interaction with the sulfonate groups. Human umbilical vein endothelial cells (HUVECs) formed focal adhesion complexes on RGD- and RGD and bFGF-immobilized patterns as shown by immunostaining of vinculin and actin. In the presence of both bFGF and RGD, cell areas were larger. The data demonstrate confinement of cellular focal adhesions to chemically and physically well-controlled microenvironments created by a combination of e-beam lithography and click chemistry techniques. The results also suggest positive implications for addition of growth factors into adhesive patterns for cell-material interactions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据