Article
Biochemistry & Molecular Biology
Angel Santorelli, Kurt Gothelf
Summary: Labelling of oligonucleotides is crucial in life sciences, and we propose a simple and cost-effective method to introduce modifications to oligonucleotides during solid phase synthesis without the need for special phosphoramidites. This method is compatible with current automated oligonucleotide synthesis and serves as a simple alternative to the unstable and expensive special phosphoramidites currently used for conjugation.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Organic
Zehong Liang, Petja Rosenqvist, Ella Pajuniemi, Mikko Ora, Petri Heinonen, Pasi Virta, Mikko Oivanen
Summary: 5'-O-(2-isopropoxyprop-2-yl) (IIP)-protection was introduced to 5'-OH function of nucleosides in high yields by an acid-catalysed transacetalization with 2,2-diisopropoxypropane. The applicability of this temporal 5'-O-protecting group was demonstrated in the liquid phase oligonucleotide synthesis (LPOS) using the corresponding phosphoramidite building blocks (dA, dG, dC and dT) and a tetrapodal precipitative soluble support. The IIP was shown to be a capable choice to the 5'-O protection in solution phase synthesis. It could be readily removed with formic acid (t(1/2)<10 s in 6 % HCOOH in dichloromethane/methanol (2/1) at RT), resulting in volatile byproducts (acetone and isopropanol).
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ivana Jestrabova, Lenka Postova Slavetinska, Michal Hocek
Summary: We designed and synthesized a series of novel nucleoside phosphoramidites for the solid-phase synthesis of modified oligonucleotides. The hybridization properties of these modified oligonucleotides were studied, and they have potential for the synthesis of highly modified functional nucleic acid-based polymers.
Article
Chemistry, Organic
Mayumi Takahashi, Andrzej Grajkowski, Brian M. Cawrse, Serge L. Beaucage
Summary: The implementation of protecting groups for the 2'-hydroxyl function of ribonucleosides remains challenging for RNA purification for therapeutic applications, requiring easy installation and efficient incorporation while minimizing impurities. Complete cleavage of the protecting group without the formation of side products or mutagenic adducts is crucial.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Ivan Sanchez-Campillo, Judit Miguel-Gracia, Periklis Karamanis, Juan B. Blanco-Canosa
Summary: Chemical protein synthesis is a consolidated field that utilizes the high chemospecificity of amide-forming reactions, particularly the native chemical ligation (NCL), alongside new technologies such as the Ser/Thr ligation and the alpha-ketoacid-hydroxylamine (KAHA) condensation. The NCL was initially designed for the ligation of peptides with a C-terminal thioester and an N-terminal cysteine. This study combines the Nbz and Bt approaches in a versatile linker, 1,2-diaminobenzene (Dbz), demonstrating high yields of ligation and the potential for transseleoesterification reactions. The results indicate faster NCL kinetics with Bt precursors, enabling rapid transthioesterification.
Article
Materials Science, Multidisciplinary
Pandian Paulraj, Kalimuthu Rajendran, Arumugam Sathamraja, Kannaiyan Pandian
Summary: Among the pi-conjugate conducting polymers, polyaniline derivatives have received much attention due to their efficient applications. In this study, Poly(o-anisidine) and POA protected Silver nanoparticles nanocomposites were successfully synthesized using a solid phase mechanochemical approach, and their properties were thoroughly analyzed. The newly fabricated POA@Ag/GCE electrode showed high stability and sensitivity in detecting dopamine, with excellent selectivity towards dopamine in the presence of additional electroactive agents.
MATERIALS TODAY COMMUNICATIONS
(2021)
Review
Engineering, Environmental
Mukesh Bhatt, Anand Gupta Chakinala, Jyeshtharaj B. Joshi, Anita Sharma, K. K. Pant, Kalpit Shah, Abhishek Sharma
Summary: Global economic development and rising living standards have led to resource depletion and increased waste generation, making waste management critical. Thermo-chemical conversion of solid waste into fuel and value-added products is important for sustainable development, but challenges posed by the heterogeneous nature of the waste must be addressed for effective implementation.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Ziming Bao, Tengwei Li, Jianzhao Liu
Summary: The natural modifications of mRNA in living organisms play essential roles in physiology and pathology. Chemical sequencing technology is necessary for mapping both natural modifications and nucleoside tags on mRNA at single base resolution, as well as for studying RNA dynamics in a pulse-chase manner through metabolic labeling.
Article
Chemistry, Medicinal
Annalaura Brai, Claudia Immacolata Trivisani, Federica Poggialini, Claudia Pasqualini, Chiara Vagaggini, Elena Dreassi
Summary: Globalization, global warming, and population aging have contributed to the spread of emerging viruses. The COVID-19 pandemic has highlighted the need for new broad-spectrum antiviral agents. DDX3X helicase has been identified as a host factor required for viral replication and can be targeted as a broad-spectrum antiviral drug.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Zoltan Kupihar, Gyorgyi Ferenc, Vencel L. Petrovicz, Viktoria R. Fay, Lajos Kovacs, Tamas A. Martinek, Zsofia Hegedus
Summary: Oligonucleotide conjugates are versatile scaffolds that can be used in various applications, such as DNA-based screening platforms and therapeutics. Different chemical approaches are available for functionalizing oligonucleotides, usually at the 5' or 3' end. Modifying oligonucleotides in the middle of the sequence allows for ligation and the creation of DNA strands with multiple ligands. In this study, a complete workflow was established for the synthesis and application of thiol-modified thymidine nucleoside phosphoramidite to prepare ligatable oligonucleotide conjugates. The conjugations were performed in solution and on solid phase, resulting in multivalent oligonucleotides decorated with tissue-targeting peptides through templated ligation.
Article
Chemistry, Multidisciplinary
Xiaona Li, You Yang
Summary: Automated chemical solid-phase synthesis is a reliable and efficient automation platform for synthesizing glycans. Automated Glycan Assembly (AGA), as a key breakthrough in the field, has evolved from a proof-of-concept to a robust technology for streamlined production of various glycans. In addition, HPLC-assisted automated synthesis shows promising potential in accessing glycans.
CHINESE JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Applied
Zhenglian Mo, Yuehong Pang, Lihong Yu, Xiaofang Shen
Summary: The study developed a method for detecting trace E2 in milk samples using COFLZU1 and Nafion coated stainless steel wire for SPME fiber preparation. The stability of the SPME fiber was improved with the protection of dialysis membrane, resulting in only 7% reduction in extraction efficiency after 160 repeated uses.
Article
Chemistry, Multidisciplinary
Anja Bluemler, Harald Schwalbe, Alexander Heckel
Summary: In this study, a multi-cycle chemoenzymatic synthesis of modified RNA with simplified solid-phase handling was developed to overcome the size limitations of RNA synthesis. The successful introduction of light-controllable RNA nucleotides was confirmed, and the method allows for modifications in the RNA backbone and introductions of photocaged and photoswitchable nucleotides, as well as photocleavable strand breaks and fluorophores.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Physical
Sol Hui Park, Ye Yeong Hwang, Inho Park, Ho Bum Park, Yun Jung Lee
Summary: This study demonstrates a solution-based strategy for highly efficient bromine doping in Li3PS4 (LPS) solid-state electrolyte, leading to significantly improved Li+ ion conductivity and cycling stability. The proposed approach shows great potential for fabricating high-performance sulfide solid-state electrolytes for large-scale applications.
ENERGY STORAGE MATERIALS
(2023)
Article
Environmental Sciences
Mariella Baratti, Sara Pinosio, Massimo Gori, Stefano Biricolti, Giacomo Chini, Sara Fratini, Stefano Cannicci, Ilaria Caliani, Matteo Oliva, Lucia De Marchi, Carlo Pretti
Summary: The study revealed significant differences in chemical composition and gene expression patterns of marbled crabs between Livorno Harbour and Secche della Meloria Marine Protected Area, reflecting responses to contaminant exposure.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
Tivadar Kiss, Andrea Szabo, Gabor Oszlanczi, Anita Lukacs, Zoltan Timar, Laszlo Tiszlavicz, Dezso Csupor
Article
Chemistry, Medicinal
Jozsef Huszar, Zoltan Timar, Krisztina Katalin Szalai, Gyoergy Keserue, Ferenc Fueloep, Botond Penke
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2008)
Article
Biochemistry & Molecular Biology
Jozsef Huszar, Zoltan Timar, Ferenc Bogar, Botond Penke, Robert Kiss, Krisztina Katalin Szalai, Eva Schmidt, Andrea Papp, Gyorgy Keserue
JOURNAL OF PEPTIDE SCIENCE
(2009)
Article
Biochemical Research Methods
Zoltan Kupihar, Zoltan Timar, Zsuzsanna Darula, Douglas J. Dellinger, Marvin H. Caruthers
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2008)
Article
Neurosciences
Franciska Erdo, Ildiko Nagy, Beata Toth, Annamaria Bui, Eva Molnar, Zoltan Timar, Remi Magnan, Peter Krajcsi
BRAIN RESEARCH BULLETIN
(2017)
Article
Pharmacology & Pharmacy
Kitti Szabo, Zoltan Nagy, Viktoria Juhasz, Joseph K. Zolnerciks, Attila Csorba, Zoltan Timar, Eva Molnar, Petra Padar, William Johnson, Erzsebet Beery, Peter Krajcsi
DRUG METABOLISM AND PHARMACOKINETICS
(2017)
Article
Chemistry, Multidisciplinary
C Hetényi, G Paragi, U Maran, Z Timár, M Karelson, B Penke
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2006)
Article
Biochemistry & Molecular Biology
Z Kupihar, Z Timar, DJ Dellinger, MH Caruthers
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
(2005)