期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 133, 期 50, 页码 20335-20340出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja2071362
关键词
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资金
- NIH [GM053640]
- NSF [MCB-0919860]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0919860] Funding Source: National Science Foundation
The time scale for ordering of the polypeptide backbone relative to the side chains is a critical issue in protein folding. The interplay between ordering of the backbone and ordering of the side chains is particularly important for the formation of beta-sheet structures, as the polypeptide chain searches for the native stabilizing cross-strand interactions. We have studied these issues in the N-terminal domain of protein L9 (NTL9), a model protein with mixed alpha/beta structure. We have developed a general approach for introducing site-specific IR probes for the side chains (azide) and backbone ((13)C=(18)O) using recombinant protein expression. Temperature-jump time-resolved IR spectroscopy combined with site-specific labeling enables independent measurement of the respective backbone and side-chain dynamics with single residue resolution. We have found that side-chain ordering in a key region of the beta-sheet structure occurs on a slower time scale than ordering of the backbone during the folding of NTL9, likely as a result of the transient formation of non-native side-chain interactions.
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