Article
Chemistry, Physical
Fangyu Jin, Shiqin Liao, Qingqing Wang, Huiying Shen, Chenyu Jiang, Jiawen Zhang, Qufu Wei, Reza A. Ghiladi
Summary: In this study, a scalable and facile method to immobilize long persistent phosphor and photosensitizer onto cotton fabric was reported. The resulting composite material exhibited superior antibacterial activity against Staphylococcus aureus and methicillin-resistant S. aureus under visible-light illumination. Mechanistic studies showed enhanced singlet oxygen production, resulting in effective pathogen inactivation. In addition, the composite fabric possessed excellent abrasion resistance, mechanical strength, and color fastness. These findings suggest the potential of using long persistent phosphors in composite fabrics for infection control applications.
APPLIED SURFACE SCIENCE
(2022)
Article
Chemistry, Applied
Huiying Shen, Shiqin Liao, Chenyu Jiang, Jiawen Zhang, Qufu Wei, Reza A. Ghiladi, Qingqing Wang
Summary: A study was conducted on the development of multifunctional water purification materials capable of degrading organic pollutants and simultaneously inactivating microorganisms in contaminated wastewater. The nanocomposite BC/MoS2 exhibited photocatalytic activity generating center dot OH from H2O2, as well as photodynamic/photothermal mechanisms, synergistically degrading pollutants and inactivating bacteria. The BC/MoS2 also showed excellent photodegradation activity for common aqueous contaminants, demonstrating potential applications in wastewater treatment and microorganism disinfection.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Multidisciplinary
Tadeo Moreno-Chicano, Leiah M. Carey, Danny Axford, John H. Beale, R. Bruce Doak, Helen M. E. Duyvesteyn, Ali Ebrahim, Robert W. Henning, Diana C. F. Monteiro, Dean A. Myles, Shigeki Owada, Darren A. Sherrell, Megan L. Straw, Vukica Srajer, Hiroshi Sugimoto, Kensuke Tono, Takehiko Tosha, Ivo Tews, Martin Trebbin, Richard W. Strange, Kevin L. Weiss, Jonathan A. R. Worrall, Flora Meilleur, Robin L. Owen, Reza A. Ghiladi, Michael A. Hough
Summary: Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, but can present challenges, especially for metalloenzymes. This study describes and compares the structures of a multifunctional globin, dehaloperoxidase B (DHP-B), obtained using different methods of room-temperature crystallographic structure determination. The results show the differences in sample requirements, data-collection time, and potential for radiation damage between these methods.
Article
Biochemistry & Molecular Biology
Nikhila Kashyap Dhanvantari Madhuresh, Hilbert Nguyen, Stefan Franzen
Summary: The pH-dependent peroxidase activity of dehaloperoxidases A and B was studied. Both isozymes followed the peroxidase ping-pong kinetic model at pH 7.0, but showed deviation at lower pH values and higher TCP concentrations due to multiple processes.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
C. V. Popescu, Thanhminh Dinh, Hongli Chen, Danielle Miller, Anastasia Washburn, Ashlyn McGuire, Rania Dumarieh, Jennifer D'Antonio, Reza A. Ghiladi
Summary: Dehaloperoxidase (DHP) is a multi-functional catalytic globin found in a marine worm. Its physiological functions include oxygen transport and oxidation of toxic substances. The electronic structure of DHP varies in different states, with pH playing a role in modulating its electron-donating properties.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Dongju Yun, Vesna de Serrano, Reza A. Ghiladi
Summary: Dehaloperoxidase (DHP) from the marine polychaete Amphitrite ornata is a multifunctional enzyme that can catalyze the oxidation of bisphenols and biphenols. The reactivity of DHP B with bisphenol A (BPA) and related compounds was studied using HPLC and GC-MS/LC-MS. The results showed that DHP B can oxidize BPA to produce cleavage products and oligomers. The crystal structures of DHP bound with different substrates were determined, and a reaction mechanism was proposed based on stopped-flow UV-visible spectroscopy. This study provides evidence of how infaunal invertebrates contribute to the biotransformation of marine pollutants.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Talita Malewschik, Leiah M. Carey, Vesna de Serrano, Reza A. Ghiladi
Summary: This study shows that the multifunctional catalytic globin dehaloperoxidase (DHP) from the marine worm Amphitrite ornata can catalyze the oxidation of various dihalophenols, and the binding positions and number of halogens play a role in the activity of DHP.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Heather R. Lucas, Eunsuk Kim, Reza A. Ghiladi
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Biographical-Item
Biochemistry & Molecular Biology
Heather R. Lucas, Eunsuk Kim, Reza A. Ghiladi
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Materials Science, Biomaterials
Wen-wen Deng, Chuan-ru Zang, Qiu-chen Li, Bo Sun, Xue-ping Mei, Lu Bai, Xin-miao Shang, Ying Deng, Ya-qian Xiao, Reza A. Ghiladi, George H. Lorimer, Xue-ji Zhang, Jun Wang
Summary: Biomass carbon dots derived from broccoli water extracts exhibit excellent antioxidant activity and biocompatibility, with potential applications in nanomedicine. The abundant C=C, carbonyl, and amino groups on their surface contribute to their outstanding antioxidant properties. These green and low-toxicity CDs effectively scavenge reactive oxygen species and possess anti-inflammatory activities.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Chemistry, Applied
Wen-wen Deng, Xue-ping Mei, Zi-jun Cheng, Tian-xiang Gan, Xuan Tian, Jiang-nan Hu, Chuan-ru Zang, Bo Sun, Jing Wu, Yin Deng, Reza. A. Ghiladi, George H. Lorimer, Gizem Keceli, Jun Wang
Summary: A novel method based on salting-out assisted deep eutectic solvent (DES) has been developed for efficient extraction of sulforaphane (SFN) from broccoli. The method showed excellent extraction efficiency, with high recovery rates achieved using activated carbon. The underlying mechanism for SFN extraction by DES was also revealed.
Article
Biochemistry & Molecular Biology
Mst Sharmin Aktar, Vesna de Serrano, Reza Ghiladi, Stefan Franzen
Summary: This study compares the binding and reactivity of 2,4-dichlorophenol (DCP) in two isoforms of dehaloperoxidase-hemoglobin (DHPA and DHPB). DCP is widely used in the chemical industry, present in waste streams, and has particular reactivity to form toxic compounds. The results show that DHPB has 3.5 times higher catalytic efficiency for DCP oxidation than DHPA by a peroxidase mechanism, but both isoforms exhibit self-inhibition at low concentrations of DCP, similar to the inhibition of 2,4,6-trichlorophenol (TCP) at higher concentration.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Chemistry, Medicinal
Li Wu, Ning Yang, Meng Guo, Didi Zhang, Reza A. Ghiladi, Hasan Bayram, Jun Wang
Summary: Sound vibration is a natural stimulus that triggers physiological changes in plants, leading to the production of secondary metabolites. This review explores the potential role of sound stimulation in regulating the biosynthesis of secondary metabolites and the subsequent physiological changes in plants, focusing on transcriptional and epigenetic regulation. Various factors, such as plant species, growth stage, sound types, frequency, intensity, and exposure time, can influence the production of secondary metabolites. The review also discusses the biochemical and physiological changes induced by sound stimulation, as well as the limitations of previous studies and the future application of sound waves in plant secondary metabolite biosynthesis.
NATURAL PRODUCTS AND BIOPROSPECTING
(2023)
Article
Chemistry, Physical
Kacie M. Wells, Yusuf Ciftci, Bharadwaja S. T. Peddinti, Reza A. Ghiladi, Govindsamy Vediyappan, Richard J. Spontak, Revathi Govind
Summary: This study found that a self-disinfecting anionic block polymer can rapidly inactivate drug-resistant and transmissible healthcare pathogens such as C. difficile and C. auris on a solid surface. The polymer generates a highly acidic (pH < 1) surface layer upon hydration, allowing proton migration from the film's interior to the surface through contiguous nanoscale hydrophilic channels. The polymer effectively inactivates C. difficile and Candida, preventing the spread of these potentially dangerous microbes in healthcare facilities.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Materials Science, Biomaterials
Hua Tian, Jixuan Hong, Changgui Li, Yuheng Qiu, Mengting Li, Ziyu Qin, Reza A. Ghiladi, Xueqiong Yin
Summary: As bacterial infections continue to pose a global healthcare challenge, new therapeutic strategies are needed. This study confirms the potential of photothermal therapy (PTT) as a non-chemotherapeutic approach to combat bacterial infections. The use of Au@CD composite nanoparticles as a photothermal agent demonstrated excellent antimicrobial activity and accelerated wound healing, indicating promising applications in healthcare.
BIOMATERIALS ADVANCES
(2022)