Article
Cell Biology
Pijus K. Barman, Juliana E. Shin, Sloan A. Lewis, Seokjo Kang, Di Wu, Yizhou Wang, Xiaoming Yang, Prakash S. Nagarkatti, Mitzi Nagarkatti, Ilhem Messaoudi, Berenice A. Benayoun, Helen S. Goodridge
Summary: Aging is associated with increased monocyte production as well as altered function, with classical monocytes in old mice showing higher expression of DCMo signature genes. This leads to increased production of moDC in old mice. Additionally, higher expression of Aw112010 in old monocytes may be linked to elevated MHCII expression, indicating a potential mechanism for altered monocyte function during aging.
Article
Medicine, Research & Experimental
Antigona Ulndreaj, Angela Li, Yonghong Chen, Rickvinder Besla, Shaun Pacheco, Marwan G. Althagafi, Myron I. Cybulsky, Thomas Lindsay, Clinton S. Robbins, John S. Byrne
Summary: The study found that aortic macrophage accumulation in abdominal aortic aneurysm (AAA) is primarily caused by adventitial recruitment of Lyve-1(-) macrophages, with a relative depletion of Lyve-1(+) macrophages. Selective targeting of macrophage subtypes may represent a potential novel therapeutic avenue for the medical treatment of AAA.
Article
Biotechnology & Applied Microbiology
Anri Koyanagi, Iichiroh Onishi, Karin Muraoka, Ikue Sato, Shingo Sato, Tsuyoshi Kimura, Akio Kishida, Kouhei Yamamoto, Masanobu Kitagawa, Morito Kurata
Summary: Hematopoiesis is maintained by the interaction of hematopoietic stem cells and bone marrow mesenchymal stem cells in bone marrow microenvironments. Certain genetic mutations in the mesenchymal stem cells can lead to hematopoietic neoplasms. This study used a specific gene mutation in human mesenchymal stem cells and a bone scaffold to create an in vivo bone marrow microenvironment model, providing insights into these interactions and disease onset.
BIOENGINEERING-BASEL
(2022)
Article
Biology
Parastoo Boroumand, David C. Prescott, Tapas Mukherjee, Philip J. Bilan, Michael Wong, Jeff Shen, Ivan Tattoli, Yuhuan Zhou, Angela Li, Tharini Sivasubramaniyam, Nancy Shi, Lucie Y. Zhu, Zhi Liu, Clinton Robbins, Dana J. Philpott, Stephen E. Girardin, Amira Klip
Summary: During obesity and high fat-diet feeding, there is an increase in pro-inflammatory macrophages in adipose tissue as well as invasive Ly6C(high) monocytes produced by the bone marrow. This study explores the relationship between bone marrow adipocytes and Ly6C(high) monocytes, and finds that the whitening of bone marrow adipocytes precedes the rise of adipose tissue macrophages during high fat-diet feeding in mice. Ex vivo treatment of bone marrow cells with conditioned media from bone marrow adipocytes or white adipocytes also favors the presence of Ly6C(high) monocytes. Furthermore, there is a metabolic reprogramming and mitochondrial fission in monocytes before the shift towards Ly6C(high) phenotype.
Article
Cell Biology
Annamaria Aprile, Laura Raggi, Simona Bolamperti, Isabella Villa, Mariangela Storto, Gaia Morello, Sarah Marktel, Claudio Tripodo, Maria Domenica Cappellini, Irene Motta, Alessandro Rubinacci, Giuliana Ferrari
Summary: Clinical evidence suggests a relationship between blood and bone, but the underlying mechanism is not fully understood. A study using beta-thalassemia as a model found that increased fibroblast growth factor 23 (FGF23) levels in patients and mice with beta-thalassemia were induced by erythropoietin via ERK1/2 and STAT5 pathways. Inhibiting FGF23 signaling with carboxyl-terminal FGF23 peptide rescued bone defects and restored hematopoietic stem cell function in mice with beta-thalassemia. FGF23 may serve as a molecular link connecting anemia, bone, and the hematopoietic stem cell niche.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Annamaria Aprile, Laura Raggi, Simona Bolamperti, Isabella Villa, Mariangela Storto, Gaia Morello, Sarah Marktel, Claudio Tripodo, Maria Domenica Cappellini, Irene Motta, Alessandro Rubinacci, Giuliana Ferrari
Summary: This study demonstrates the overproduction of fibroblast growth factor 23 (FGF23) in patients and mice with beta-thalassemia, and shows that inhibition of FGF23 signaling is a safe and effective therapeutic strategy to rescue bone defects and restore hematopoietic stem cell function. FGF23 may serve as a molecular link connecting anemia, bone, and the HSC niche.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Engineering, Biomedical
Yinbo Xiao, ChanelleA. S. McGuinness, W. Sebastian Doherty-Boyd, Manuel Salmeron-Sanchez, Hannah Donnelly, Matthew J. Dalby
Summary: Hematopoietic stem cells play a fundamental role in the generation of blood and immune cells. Although the signaling mechanisms in their bone marrow niche microenvironment have been increasingly understood, the ability to recreate these mechanisms in vitro for clinical purposes is still lacking. Recent studies have used engineering techniques to establish ex vivo bone marrow niche models, showing promising potential for research and clinical applications.
Article
Multidisciplinary Sciences
Runfeng Miao, Harim Chun, Xing Feng, Ana Cordeiro Gomes, Jungmin Choi, Joao P. Pereira
Summary: This study reveals that a complex homeostatic balance between hematopoietic stem cells (HSCs) and hematopoietic progenitor cells is maintained through competition for a limited amount of cell signaling molecule. When early hematopoietic progenitors fail to interact with specific cells, HSC numbers increase.
NATURE COMMUNICATIONS
(2022)
Article
Hematology
Gustavo Falero-Diaz, Catarina de A. Barboza, Felipe Pires, Maeva Fanchin, Jingjing Ling, Zachary M. Zigmond, Anthony J. Griswold, Laisel Martinez, Roberto Vazquez-Padron, Omaida C. Velazquez, Roberta M. Lassance-Soares
Summary: This study found that functional reprogramming of monocytes after ischemia training improves blood flow and arteriogenesis after hindlimb ischemia. The changes in metabolism and gene expression may contribute to this beneficial effect.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2022)
Article
Hematology
Nagham Alouche, Amelie Bonaud, Vincent Rondeau, Rim Hussein-Agha, Julie Nguyen, Valeria Bisio, Melanie Khamyath, Etienne Crickx, Niclas Setterblad, Nicolas Dulphy, Matthieu Mahevas, David H. McDermott, Philip M. Murphy, Karl Balabanian, Marion Espeli
Summary: Enhanced Cxcr4 signaling due to defective receptor desensitization leads to exacerbated extrafollicular B-cell response, as demonstrated in a mouse model bearing a gain-of-function mutation of Cxcr4 found in human hematologic disorders. Mutant B cells exhibit enhanced mechanistic target of rapamycin signaling, cycle more, and differentiate more potently into plasma cells after Toll-like receptor stimulation. Additionally, Cxcr4 gain of function promotes enhanced homing and persistence of immature plasma cells in the bone marrow, resulting in increased and more sustained antibody production after T-independent immunization.
Review
Biochemistry & Molecular Biology
Kathryn A. Skelding, Daniel L. Barry, Danielle Z. Theron, Lisa F. Lincz
Summary: Acute myeloid leukaemia (AML) has a low survival rate and its resistance to traditional therapies has led to the exploration of the bone marrow microenvironment as a potential source of therapeutic targets. This review discusses the role of the microenvironment in AML development and progression, and focuses on drugs that modulate or target this niche in in vivo models or clinical studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Endocrinology & Metabolism
Matthew J. Kim, Rodrigo J. Valderrabano, Joy Y. Wu
Summary: This article discusses various populations of osteoblasts at different stages of differentiation and summarizes the role of the osteoblast lineage in supporting hematopoiesis.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Nicole Pui-Yu Ho, Hitoshi Takizawa
Summary: This article summarizes the current understanding of how hematopoietic stem cell functions are maintained, damaged, or exhausted during acute, prolonged, and pathological inflammatory conditions. It also highlights the impact of inflammation-altered hematopoietic stem cell niche on escalating the insults on hematopoietic stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Giulia Pozzi, Cecilia Carubbi, Giuliana Gobbi, Sara Tagliaferri, Prisco Mirandola, Marco Vitale, Elena Masselli
Summary: The detection of CCR2(+)CD34(+) cells by flow cytometry has good diagnostic accuracy in distinguishing between different MPN subtypes with varying degrees of bone marrow fibrosis, particularly for differentiating between ET and prePMF as well as between prePMF and overtPMF.
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Siddharth Krishnan, Kelly Wemyss, Ian E. Prise, Flora A. McClure, Conor O'Boyle, Hayley M. Bridgeman, Tovah N. Shaw, John R. Grainger, Joanne E. Konkel
Summary: HSPCs were found to reside and differentiate in healthy gingiva, a key oral barrier. Gingival HSPCs exhibited similar characteristics to bone marrow HSPCs but responded differently to inflammatory insults, only responding to oral rather than systemic inflammation.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Neurosciences
Somang Kang, Chul-Hoon Kim, Hosung Jung, Eosu Kim, Ho-Taek Song, Jong Eun Lee
Article
Biochemistry & Molecular Biology
Hyeshik Chang, Jinah Yeo, Jeong-gyun Kim, Hyunjoon Kim, Jaechul Lim, Mihye Lee, Hyun Ho Kim, Jiyeon Ohk, Hee-Yeon Jeon, Hyunsook Lee, Hosung Jung, Kyu-Won Kim, V. Narry Kim
Article
Cell Biology
Incheol Ryu, You-Sub Won, Hongseok Ha, Eunjin Kim, Yeonkyoung Park, Min Kyung Kim, Do Hoon Kwon, Junho Choe, Hyun Kyu Song, Hosung Jung, Yoon Ki Kim
Article
Cell Biology
Toshiaki Shigeoka, Max Koppers, Hovy Ho-Wai Wong, Julie Qiaojin Lin, Roberta Cagnetta, Asha Dwivedy, Janaina de Freitas Nascimento, Francesca W. van Tartwijk, Florian Strohl, Jean-Michel Cioni, Julia Schaeffer, Mark Carrington, Clemens F. Kaminski, Hosung Jung, William A. Harris, Christine E. Holt
Review
Neurosciences
Eunjin Kim, Hosung Jung
CURRENT OPINION IN NEUROBIOLOGY
(2020)
Article
Oncology
Hyunji Lee, Hyunjung Chin, Hyeyoung Kim, Hosung Jung, Daekee Lee
MOLECULAR ONCOLOGY
(2020)
Article
Clinical Neurology
Joohyun Park, Jong Youl Kim, Yu Rim Kim, Meiying Huang, Ji Young Chang, A. Young Sim, Hosung Jung, Won Taek Lee, Young-Min Hyun, Jong Eun Lee
Summary: After ischemic stroke, CCR2(high)CX3CR1(low) monocytes recruited to the injured brain are cytokine-dependently converted into CCR2(low)CX3CR1(high) macrophages, attenuating neuroinflammation. The regulation of monocyte-switching is crucial in ischemic stroke, and the adaptation of monocytes in a locally inflamed milieu is vital in alleviating the effects of stroke through innate immunity.
TRANSLATIONAL STROKE RESEARCH
(2021)
Article
Neurosciences
Chunmei Jin, Yeunkum Lee, Hyojin Kang, Kwon Jeong, Joori Park, Yinhua Zhang, Hyae Rim Kang, Ruiying Ma, Hyunyoung Seong, Yoonhee Kim, Hosung Jung, Jin Young Kim, Yoon Ki Kim, Kihoon Han
Summary: The study suggests that Shank3 may remodel the postsynaptic proteome by regulating synaptic protein synthesis, and dysfunction in this process could be implicated in SHANK3-associated synaptopathies.
Article
Multidisciplinary Sciences
Min Ji Yoon, Boyoon Choi, Eun Jin Kim, Jiyeon Ohk, Chansik Yang, Yeon-Gil Choi, Jinyoung Lee, Chanhee Kang, Hyun Kyu Song, Yoon Ki Kim, Jae-Sung Woo, Yongcheol Cho, Eui-Ju Choi, Hosung Jung, Chungho Kim
Summary: The study reveals that UXT functions as an autophagy adaptor for p62-dependent aggrephagy, delaying motor neuron degeneration in a Xenopus model. Additionally, the cooperative relationship between molecular chaperones and the aggrephagy machinery is demonstrated for efficient removal of misfolded protein aggregates.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Hyun Jung Park, Hosung Jung
Summary: Recent technological advances in single-cell and single-nucleus transcriptomics have allowed for a detailed examination of neuro-immune interactions in both healthy and diseased brains. This review provides an overview of literature that investigates gene expression in inflamed brains at the single-cell level, focusing on areas such as maternal immune activation, viral infection, and auto-immune diseases. The reviewed articles also offer strong evidence for the involvement of the immune system in neurodegenerative diseases, which are not traditionally considered as neuroinflammatory. Additionally, this review includes a diagram of experimental and analytical processes in single-cell analysis of the brain and discusses recurring themes and potential future research directions in neuro-immune interactions.
ANIMAL CELLS AND SYSTEMS
(2022)
Article
Neurosciences
Jane Jung, Jiyeon Ohk, Hyeyoung Kim, Christine E. Holt, Hyun Jung Park, Hosung Jung
Summary: In this study, a method for capturing the axonal transcriptome was developed, allowing deep sequencing of metabolically labeled mRNAs in retinal ganglion cell axon terminals in mice. The comparison of axonal-to-somal transcriptomes and axonal translatome-to-transcriptome provides a genome-wide visualization of mRNA transport and translation and identifies potential regulators for each process, including FMRP and TDP-43. Experiments in knockout mice confirm the role of FMRP in axonal transportation of synapse-related mRNAs. Pulse-and-chase experiments reveal a strong coupling between mRNA translation and decay. The absolute mRNA abundance per axon terminal remains stable due to persistent transport. These findings provide valuable insights into RNA-based mechanisms in maintaining presynaptic structure and function in vivo.
Article
Medicine, Research & Experimental
Minjee Kim, Hyeyoung Kim, Bu-Gyeong Kang, Jooyoung Lee, Taegun Kim, Hwanho Lee, Jane Jung, Myung Joon Oh, Seungyoon Seo, Myung-Jeom Ryu, Yeojin Sung, Yunji Lee, Jeonghun Yeom, Gyoonhee Han, Sun-Shin Cha, Hosung Jung, Hyun Seok Kim
Summary: This study identifies a novel NAMPT inhibitor, A4276, that selectively targets NAPRT-deficient EMT-subtype cancer cells and prevents chemotherapy-induced peripheral neuropathy. It highlights the potential of A4276 as a promising anti-cancer agent for use in cancer monotherapy or combination therapy with conventional chemotherapeutics.
Article
Biochemistry & Molecular Biology
Boyoon Choi, Hyeyoung Kim, Jungim Jang, Sihyeon Park, Hosung Jung
Summary: Neurons form long-distance connections through their axons, and the accuracy and stability of these connections are crucial for brain function. Investigating the molecular and cellular mechanisms underlying neuronal circuit assembly and maintenance requires an efficient vertebrate model. Xenopus tropicalis, a diploid amphibian species, is an ideal model for visualizing axonal pathfinding and degeneration of a specific neuronal cell type - the retinal ganglion cell. This study demonstrates that the visual system of X. tropicalis is highly efficient for identifying new molecular mechanisms underlying axon guidance and survival.
MOLECULES AND CELLS
(2022)
Article
Medicine, Research & Experimental
Jae Young Kim, Jong Youl Kim, Jae Hwan Kim, Hosung Jung, Won Taek Lee, Jong Eun Lee
EXPERIMENTAL NEUROBIOLOGY
(2019)
Article
Clinical Neurology
Yongjin Yoo, Jane Jung, Yoo-Na Lee, Youngha Lee, Hyosuk Cho, Eunjung Na, JeaYeok Hong, Eunjin Kim, Jin Sook Lee, Je Sang Lee, Chansik Hong, Sang-Yoon Park, Jinhong Wie, Kathryn Miller, Natasha Shur, Cheryl Clow, Roseanne S. Ebel, Suzanne D. DeBrosse, Lindsay B. Henderson, Rebecca Willaert, Christopher Castaldi, Irina Tikhonova, Kaya Bilguvar, Shrikant Mane, Ki Joong Kim, Yong Seung Hwang, Seok-Geun Lee, Insuk So, Byung Chan Lim, Hee-Jung Choi, Jae Young Seong, Yong Beom Shin, Hosung Jung, Jong-Hee Chae, Murim Choi
ANNALS OF NEUROLOGY
(2017)