Article
Plant Sciences
Abujunaid Khan, Madhukar S. Said, Balasaheb R. Borade, Rajesh Gonnade, Vitthal Barvkar, Ravindar Kontham, Syed G. Dastager
Summary: Three novel compounds and a new acid were identified from the strain Amycolatopsis sp. MCC 0218, and their chemical structures and activities against methicillin-susceptible and -resistant Staphylococcus aureus were determined.
JOURNAL OF NATURAL PRODUCTS
(2022)
Article
Microbiology
Mingli Liu, Ruiqin Hu, Wenhao Li, Wenyi Yang, Qianghua Xu, Liangbiao Chen
Summary: This study confirmed the formation process of mature hepcidins in D. mawsoni and evaluated their antibacterial activity. The results indicated that hepcidin plays an important role in the innate immunity of D. mawsoni and is of great value in improving resistance to pathogens.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shu-Ping Zou, Xin Han, Han-Yue Zhu, Qi Sheng, Heng Tang, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: This study demonstrated the functional expression of Echinocandin B deacylase (ECBD) in Escherichia coli (E. coli) for the first time, using peptide tags to enhance solubility. Optimal pH and temperature conditions were determined through culture optimization. These results can serve as a reference for the high-level expression of other deacylases in a possible industrial application.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Huan Li, Shuang Fu, Yudan Wang, Xuan Yuan, Lijia Liu, Hongxing Dong, Qiang Wang, Zhijia Zhang
Summary: Thirteen cationic peptidomimetics derived from amino acids were designed and synthesized to mimic the structure of cationic antibacterial peptides. Most of them showed significant broad-spectrum antibacterial activity against various bacteria, with some exhibiting low cytotoxicity. These peptidomimetics may have potential for the treatment of bacterial infections.
BIOORGANIC CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Tianxi Li, Lulu Li, Fangyuan Du, Lei Sun, Jichao Shi, Miao Long, Zeliang Chen
Summary: This review focuses on antifungal peptides isolated from various microorganisms, outlining their antifungal activity, toxic effects, and mechanisms of action, as well as common solutions to mitigate their toxicity. This work serves as a useful reference for further clinical research and the development of safe antifungal drugs with high efficiencies and broad application spectra.
Article
Agriculture, Dairy & Animal Science
Thippawan Pimchan, Fu Tian, Kanjana Thumanu, Sureelak Rodtong, Jirawat Yongsawatdigul
Summary: The study aims to fractionate antibacterial peptides from pepsin-hydrolyzed egg yolks using flash chromatography and determine their mode of action. Fraction 6 (F6) exhibited antibacterial activity against Staphylococcus aureus and Salmonella typhimurium. The peptides caused DNA leakage, cell membrane disintegration, and cell ruptures, indicating their potential as antistaphylococcal agents.
Article
Food Science & Technology
Yangliu Liu, Panpan Han, Yingmin Jia, Zhou Chen, Siting Li, Aijin Ma
Summary: This study established an antibacterial activity database and investigated the application of brevilaterins, antibacterial lipopeptides from Brevibacillus laterosporus S62-9, in food. The results showed that brevilaterins exhibited broad-spectrum inhibitory and bactericidal activity, particularly against Gram-positive bacteria and spoilage bacteria in grain products. In addition, brevilaterins effectively inhibited and killed multiple antibiotic-resistant bacteria and did not readily develop resistance. The study also found that brevilaterins interacted with sodium dodecyl sulfate and soybean phospholipid. There was no significant difference in activity between brevilaterin B and brevilaterin C.
Article
Food Science & Technology
Yihan Yu, Qiuhui Hu, Jianhui Liu, Anxiang Su, Hui Xu, Xiuting Li, Qingrong Huang, Jinlan Zhou, Alfred Mugambi Mariga, Wenjian Yang
Summary: A novel immunologically active peptide KSPLY from Hericium erinaceus was obtained, which could regulate immune function by promoting the secretion of various cytokines by macrophages. In addition to inhibiting NO and IL-6 secretion by M1 macrophages, KSPLY also promoted the transformation of M2 macrophages into M1 macrophages.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Guangrong Liu, Mayila Kamilijiang, Aytursun Abuduwaili, Deng Zang, Nuermaimaiti Abudukelimu, Geyu Liu, Abulimiti Yili, Haji Akber AIsa
Summary: The optimum conditions for extracting Saussurea involucrata polysaccharide (SIP) were determined, and a new acidic polysaccharide SIP-II was isolated and characterized. SIP-II showed significant anti-inflammatory activity in LPS-stimulated macrophages, potentially making it a valuable ingredient in the functional food and pharmaceutical industry.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biochemistry & Molecular Biology
Sinan Wang, Chuang Li, Liyan Zhang, Bingxia Sun, Yuting Cui, Feng Sang
Summary: Bacterial infections are a major threat to global public health, and the abuse of antibiotics has led to the emergence of bacterial resistance. Natural chalcones, with their potent antibacterial properties, have the potential to become new treatments for infectious diseases. However, research on the antibacterial activity and mechanisms of chalcones is limited. This review focuses on seventeen natural chalcones and provides a classification based on their antibacterial mechanisms, summarizes their isolation and biological activity, and explores their antibacterial mechanisms.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Run Xu, Yongxiang Song, Jun Li, Jianhua Ju, Qinglian Li
Summary: Desotamides A4 and A6, with l-Ile at position II and d-Lys or d-Arg at position VI, exhibit enhanced antibacterial activities against a range of Gram-positive pathogens, including drug-resistant strains like MRSA. Their broad spectrum and increased potency make them promising candidates for further antibiotic development.
Article
Food Science & Technology
Xiao-Yan Zu, Wen-Bo Liu, Guang-Quan Xiong, Tao Liao, Hai-Lan Li
Summary: The antioxidant activity and physicochemical properties of swim bladder polypeptides (SBPs) from Acipenser schrencki were analyzed. The optimal conditions for enzymatic digestion were determined as alkaline protease with a solid-to-liquid ratio of 1:20, 4-hour incubation at 55 degrees C, and an enzyme dosage of 5000 U/g. Ultrafiltration yielded three fractions (F1, F2, and F3) with F3 showing the highest removal rates of O-2 center dot(-), DPPH center dot, and center dot OH. F3 contained proline, hydroxyproline, and hydrophobic amino acids, as well as antioxidant and inhibitory peptides.
Article
Microbiology
Longfeng Wei, Qinyu Zhang, Ailin Xie, Yang Xiao, Kun Guo, Shuzhen Mu, Yudan Xie, Zhu Li, Tengxia He
Summary: This study explored the bioactive compounds of Aspergillus niger and evaluated their antibacterial activity against three plant pathogens. The results showed that compound 4 exhibited the strongest antibacterial activity against Ralstonia solanacearum, suggesting its potential as a biocontrol agent.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ping Xu, Libo Yuan, Ke Wang, Boyuan Pan, Yong Ye, Kui Lu
Summary: Two peptide-carbazole conjugates, CTAT and CNLS, were synthesized and used as modifications for cell membrane penetrating peptide TAT (47-57) and nuclear localization peptide NLS. Their interactions with ctDNA were studied through multispectral and agarose gel electrophoresis. It was found that both CTAT and CNLS bind to ctDNA in a minor groove binding manner and have a tighter binding affinity compared to CIBA, TAT, and NLS. Furthermore, CTAT and CNLS can unfold parallel G-quadruplex structures and exhibit antimicrobial activity by disrupting cell membrane bilayer integrity and binding to DNA.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Zhengshi Zhang, Yongcan Zhou, Han Zhang, Xiangyu Du, Zhenjie Cao, Ying Wu, Chunsheng Liu, Yun Sun
Summary: TroHepc2-22, a derived peptide from Trachinotus ovatus hepcidin2, exhibits superior antibacterial activity against both Gram-negative and Gram-positive bacteria by inducing bacterial membrane depolarization and changing membrane permeability. It also possesses hydrolytic activity on bacterial genomic DNA. In vivo assays demonstrate that TroHepc2-22 enhances the resistance against V. harveyi infection and stimulates the expression of immune-related genes and cytokines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Mamdouh Nabil Samy, Geraldine Le Goff, Philippe Lopes, Katerina Georgousaki, Sentiljana Gumeni, Celso Almeida, Victor Gonzalez-Menendez, Olga Genilloud, Ioannis P. Trougakos, Nikolas Fokialakis, Jamal Ouazzani
Summary: Three known compounds were isolated from Virgaria nigra CF-231658, with compound 2 displaying the most potent activity in inhibiting elastase at a concentration of 5 μM. The structures of the compounds were elucidated through spectroscopic analyses, and their abilities to inhibit the enzyme activity were examined using normal human diploid fibroblasts.
NATURAL PRODUCT RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Mohammed Aldholmi, Rizwan Ahmad, Daniel Carretero-Molina, Ignacio Perez-Victoria, Jesus Martin, Fernando Reyes, Olga Genilloud, Lea Gourbeyre, Thierry Gefflaut, Hanne Carlsson, Alexei Maklakov, Ellis O'Neill, Robert A. Field, Barrie Wilkinson, Maria O'Connell, A. Ganesan
Summary: The study discovered that euglenatides from Euglena gracilis can inhibit pathogenic fungi and cancer cells, and bear resemblance to nemamides in Caenorhabditis elegans. Molecular network analysis also revealed the presence of multiple euglenatide-like metabolites in Euglena species.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Matilde Ortiz-Gonzalez, Ignacio Perez-Victoria, Inmaculada Ramirez-Macias, Nuria de Pedro, Angel Linde-Rodriguez, Victor Gonzalez-Menendez, Victoria Sanchez-Martin, Jesus Martin, Ana Soriano-Lerma, Olga Genilloud, Virginia Perez-Carrasco, Francisca Vicente, Jose Maceira, Carlos A. Rodrigues-Poveda, Jose Maria Navarro-Mari, Fernando Reyes, Miguel Soriano, Jose A. Garcia-Salcedo
Summary: Sleeping sickness is a serious health concern in sub-Saharan Africa and there is currently no vaccine available. Researchers have discovered a new compound, curvicollide D, that shows strong inhibitory effects on Trypanosoma brucei and can induce cell death.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Psychology, Developmental
Brianna Piro-Gambetti, Jessica Greenlee, Emily J. Hickey, Jennifer M. Putney, Emily Lorang, Sigan L. Hartley
Summary: Autistic youth are at risk for internalizing mental health problems, such as depression and anxiety. Parents of autistic youth also experience higher levels of depression compared to parents of typically developing children. This study reveals bidirectional associations between parent depression symptoms and the internalizing problems of autistic youth. Future research should focus on exploring the genetic and environmental pathways that link parent depression and internalizing problems in autistic youth.
JOURNAL OF AUTISM AND DEVELOPMENTAL DISORDERS
(2023)
Article
Pediatrics
William E. E. MacLean Jr, Sara R. R. Jeglum, Emily J. J. Hickey, Alexandra Ament
CLINICAL PEDIATRICS
(2023)
Article
Pharmacology & Pharmacy
Frederick Boye Annang, Guiomar Perez-Moreno, Cristina Bosch-Navarrete, Victor Gonzalez-Menendez, Jesus Martin, Thomas A. A. Mackenzie, Maria C. C. Ramos, Luis M. M. Ruiz-Perez, Olga Genilloud, Dolores Gonzalez-Pacanowska, Francisca Vicente, Fernando Reyes
Summary: Memnoniella is a fungal genus that produces a variety of biologically active compounds. A specific extract from Memnoniella dichroa CF-080171 showed potent activity against Plasmodium falciparum 3D7 and Trypanosoma cruzi Tulahuen parasites. Eight new meroterpenoids with varying potencies were isolated from the extract, and compounds 1, 2, and 4 exhibited strong antiparasitic activity against the mentioned parasites and cytotoxic activity against HepG2 cells.
Article
Microbiology
Marina Sanchez-Hidalgo, Maria Jesus Garcia, Ignacio Gonzalez, Daniel Oves-Costales, Olga Genilloud
Summary: In this study, a strain of Kribbella sp. CA-293567 was found to produce sandramycin and kribbellichelins A-B, which are new secondary metabolites. The complete genome sequencing and in silico identification of biosynthetic gene clusters were performed, focusing on the pathways encoding these compounds. A comparative analysis of the biosynthetic potential of 38 publicly available genomes from Kribbella strains was also presented.
Article
Psychology, Developmental
Jessica L. Greenlee, Emily Hickey, Claire R. Stelter, Tuyen Huynh, Sigan L. Hartley
Summary: Parents of autistic children often experience more stress and have worse mental and physical health compared to parents of neurotypical children. However, not all parents of autistic children are distressed. This study examined the different parenting profiles of 183 mothers and fathers of autistic children and found that parents in the adaptive profile, characterized by low stress, high competence, and positive parenting strategies, had children with fewer behavioral problems and less severe autism symptoms. The findings suggest the importance of understanding and supporting diverse parenting experiences in interventions for families of autistic children.
Article
Education, Special
Jocelyn Kuhn, Emily Hickey, Olivia Lindly, Michelle Stransky, Marisa Masaro, Gregory J. Patts, Howard Cabral, Morgan Crossman, Marilyn Augustyn, Emily Feinberg, Sarabeth Broder-Fingert
Summary: This study examined the relationships between child social functioning, parenting stress, and Part C Early Intervention (EI) enrollment. The results showed no direct relationship between EI enrollment and parenting stress, but a significant inverse association was found between child social functioning and parenting stress, which was moderated by EI enrollment. The findings suggest potential benefits of timely referral and access to EI for children at risk of developmental delay in historically underserved communities.
JOURNAL OF EARLY INTERVENTION
(2023)
Article
Biochemistry & Molecular Biology
Ramon I. Santamaria, Ana Martinez-Carrasco, Jose R. Tormo, Jesus Martin, Olga Genilloud, Fernando Reyes, Margarita Diaz
Summary: The co-culturing of microorganisms is a well-known strategy to study microbial interactions in the laboratory. In this study, the effects of the interaction between nine Streptomyces species and predator bacteria Myxococcus xanthus were investigated. It was found that certain Streptomyces species induce mound formation of M. xanthus on complex media. Moreover, the study identified siderophores produced by S. griseus and molecules produced by S. peucetius that affect the behavior of M. xanthus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Mary D. Slavin, Hannah M. Bailey, Emily J. Hickey, Ananya Vasudevan, Aileen Ledingham, Linda Tannenbaum, Lucinda Bateman, David L. Kaufman, Daniel L. Peterson, Ilene S. Ruhoy, David M. Systrom, Donna Felsenstein, Lewis E. Kazis
Summary: Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a chronic disease that affects a significant number of individuals in the United States. Patients experience reduced ability to engage in activities and exhibit various symptoms. Diagnosis is challenging due to the complex and fluctuating nature of the symptoms.
Article
Pharmacology & Pharmacy
Andrea Bagan, Sergio Rodriguez-Arevalo, Teresa Taboada-Jara, Christian Grinan-Ferre, Merce Pallas, Iria Brocos-Mosquera, Luis F. Callado, Jose A. Morales-Garcia, Belen Perez, Caridad Diaz, Rosario Fernandez-Godino, Olga Genilloud, Milan Beljkas, Slavica Oljacic, Katarina Nikolic, Carmen Escolano
Summary: This study aims to find molecular entities that modulate imidazoline I-2 receptors (I-2-IRs) as potential therapeutic targets for Alzheimer's disease. The findings demonstrate that the new I-2-IR ligands have beneficial effects in neuroprotection, anti-inflammation, and improvement of cognitive impairment, suggesting their potential as a promising therapeutic strategy for neurodegenerative diseases.
Article
Microbiology
Ignacio Fernandez-Pastor, Victor Gonzalez-Menendez, Kevin Martinez Andrade, Rachel Serrano, Thomas A. Mackenzie, Guillermo Benitez, Manuel Casares-Porcel, Olga Genilloud, Fernando Reyes
Summary: In this study, six species of lichens from gypsum areas in southern Spain were tested for their potential as sources of new antifungal metabolites. Three lichen extracts showed antifungal activity, and ten metabolites were identified, including a new derivative. These metabolites exhibited broad-spectrum antifungal activity against various phytopathogenic fungi.
Article
Microbiology
Rachel Serrano, Victor Gonzalez-Menendez, Jose R. Tormo, Olga Genilloud
Summary: This paper aims to develop and validate a high-throughput screening platform for evaluating the antifungal potential of chemicals and natural products against four important plant pathogens. The optimal assay conditions were determined through several experiments, and the platform was validated using known antifungal agents and microbial natural product extracts.
Article
Chemistry, Applied
Yaojie Guo, Simone Ghidinelli, Mercedes de la Cruz, Thomas A. Mackenzie, Maria C. Ramos, Pilar Sanchez, Francisca Vicente, Olga Genilloud, Thomas O. Larsen
Summary: Two new nonribosomal peptides, oxepinamides L (1) and M (2), were isolated from Aspergillus californicus IBT 16748 through chemical investigation. The structures of these compounds were characterized by spectroscopic analysis, and the absolute structure of compound 1 was determined by ECD calculation. The antibacterial and cytotoxic properties of compound 1 were evaluated.
NATURAL PRODUCT RESEARCH
(2022)