期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 130, 期 21, 页码 6813-6819出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja8004607
关键词
-
资金
- Medical Research Council [G0300122] Funding Source: researchfish
- MRC [G0300122] Funding Source: UKRI
- Medical Research Council [G0300122] Funding Source: Medline
Mustards, including sulfur mustards and nitrogen mustards, form a class of cytotoxic, vesicant chemical warfare agents. Mustards have also been used to treat cancer and played a vital role in the development of chemotherapy. Additionally, because of their destructive properties, ease of synthesis, and the lack of effective antidotes, mustards are unquestionably terrorist threats. Therefore, quick and convenient detection of mustards is a critical issue. In the present study, we achieved detection of various mustards on the basis of their chemical reactivity by using engineered alpha-hemolysin (alpha HL) protein pores as sensor elements. We describe four classes of reactions for detecting mustards. These reactions occur between mustards and thiol groups contributed by cysteine side-chains within the lumen of the alpha HL pore or on an internal molecular adapter. The approach is quick and straightforward. It can confirm the existence of mustards in as little as 10 min at 50 mu M or lower.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据