期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 130, 期 27, 页码 8594-+出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja802931q
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The active site of a Diels-Alderase ribozyme is located in solution by photoaffinity cross-linking using a productlike azidobenzyl probe. Two key nucleotides are identified that contact the Diels-Alder product in a conformation-dependent fashion. The design of such probes does not require knowledge of the three-dimensional structure of the ribozyme, and the technique yields both static and dynamic structural information. This work establishes photoaffinity cross-linking as an empirical approach that is applied here for the first time to an artificial ribozyme.
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