Review
Biotechnology & Applied Microbiology
Shiyong Huang, Yanfen Xue, Yanhe Ma, Cheng Zhou
Summary: This mini-review provides an overview of the research on IspH, an enzyme involved in the MEP pathway and isoprenoid biosynthesis. It highlights the importance of studying this enzyme for the synthesis of alternative fuels and as a potential drug target for infectious diseases.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tuniyazi Abuduaini, Vincent Roy, Julien Marlet, Catherine Gaudy-Graffin, Denys Brand, Cecile Baronti, Franck Touret, Bruno Coutard, Tamara R. McBrayer, Raymond F. Schinazi, Luigi A. Agrofoglio
Summary: Novel 1,4-disubstituted-1,2,3-triazol-(E)-2-methyl-but-2-enyl nucleosides phosphonate prodrugs with 4-substituted-1,2,3-triazoles were synthesized through olefin acyclic cross metathesis. Compound 15j, a HDP/POC prodrug, exhibited significant activity against HBV in Huh7 cell cultures, with 62% inhibition at 10 μM.
Article
Biotechnology & Applied Microbiology
Yeon Bok Kim, Sang-Min Kim, Ramaraj Sathasivam, Yong-Kyoung Kim, Sang Un Park, Soo-Un Kim
Summary: Overexpression of Ginkgo biloba HDR in transgenic tobacco results in increased chlorophyll and carotenoid levels, leading to enhanced photosynthetic rate. This is attributed to the upregulation of downstream genes involved in isoprenoid biosynthesis.
Article
Multidisciplinary Sciences
L. Matchett-Oates, S. Braich, G. C. Spangenberg, S. Rochfort, N. O. Cogan
Summary: This study proposes an in silico best practices approach for genome editing technologies in cannabis, targeting all genes involved in cannabinoid biosynthesis. By analyzing copy number variation and SNP variants in a large dataset of reference genomes, multiple online CRISPR design tools were used to design sgRNA targeting every gene, resulting in successful creation of knockout mutations for further investigation. This framework has wide applications in all plant species and could potentially lead to novel applications in agriculture and medicine by identifying SNP variants and improving genome editing processes.
Article
Chemistry, Analytical
Shixin Cai, Xin Chen, Haohan Chen, Yuting Zhang, Xiaoli Wang, Nandi Zhou
Summary: A fluorescent aptasensor for ATP detection is presented in this study, which combines a split aptamer and a DNAzyme/walker to realize fluorescence detection of ATP. The aptasensor shows a linear response range from 5 nM to 10,000 nM, with a detection limit of 0.27 nM. It has the potential application in medical samples for measuring ATP levels in human serum.
Article
Chemistry, Medicinal
Serkan Yigitkan, Abdulselam Ertas, Ramin Ekhteiari Salmas, Mehmet Firat, Ilkay Erdogan Orhan
Summary: This study investigated the HMG-CoA reductase inhibitory potential of extracts from the roots and aerial parts of Salvia multicaulis Vahl. and identified 7-acetoxyhorminone as the most effective inhibitory compound.
Article
Food Science & Technology
Min-Kyoung Kang, Ji-Young Kim, Young-Im Choi, Lujie Hu, Chaodong Yang, Zhehao Jin, Yun Ji Park, Soo-Un Kim, Sang-Min Kim
Summary: This study aims to enhance the terpenoid metabolic flux and production in plants by overexpressing the GbHDR1 gene in poplar plants, which led to accelerated growth, delayed winter-bud formation, and increased photosynthetic rate.
APPLIED BIOLOGICAL CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Jean-Alexandre Bureau, Magdalena Escobar Oliva, Yueming Dong, Codruta Ignea
Summary: This review covers the progress in producing terpenoid compounds in microbial hosts by metabolic engineering and synthetic biology approaches from 2011 to 2022, with a focus on four directions: manipulation of host metabolism, rewiring and reconstructing metabolic pathways, engineering the catalytic activity, substrate selectivity and product specificity of biosynthetic enzymes, and localizing terpenoid production via enzymatic fusions and scaffolds, or subcellular compartmentalization.
NATURAL PRODUCT REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Eunice Hui Yen Hui, Barindra Sana, Timothy Ho, Ding Ke, Farid J. Ghadessy, Hung A. Duong, Jayasree Seayad
Summary: The study introduces a novel enzymatic halogenation method, which can efficiently carry out halogenation of industrially important compounds without the need for external enzymes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jinmei Wang, Yuan Lu, Pengpeng Cheng, Chuyue Zhang, Lan Tang, Lihua Du, Jinghua Li, Zhimin Ou
Summary: In this study, bi-enzyme self-assembly clusters (BESCs) consisting of carbonyl reductase (CpCR) and glucose dehydrogenase (GDH) were synthesized. CpCR-SpyCatcher-SpyTag-GDH showed better catalytic activity and efficiency in converting ethyl 2-oxo-4-phenylbutyrate (OPBE) to ethyl (R)-2-hydroxy-4-phenylbutanoate ((R)-HPBE).
Article
Chemistry, Multidisciplinary
Yuanfan Wang, Jie Chen, Jie Yang, Zhiwei Jiao, Cheng-Yong Su
Summary: A visible light photosensitizing metal-organic cage is used as an artificial supramolecular reactor to control the reaction of aryl radicals with terminal olefins, resulting in the selective transformation of geometrically defined E/Z-alkenes. The hydrophobic cage efficiently includes aromatic substrates, promoting Meerwein arylation and protecting E-isomeric products, while benzonitrile induces efficient E -> Z isomerization. In addition to pi-pi stacking, hydrogen bonding and halogen bonding interactions play a role in controlling the arylation of aliphatic terminal olefins. This switchable cage-confined arylation catalysis has been demonstrated in the syntheses of Tapinarof and a marine natural product, achieving controllable E/Z selectivity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Agata Lipko, Cezary Paczkowski, Laura Perez-Fons, Paul D. Fraser, Magdalena Kania, Marta Hoffman-Sommer, Witold Danikiewicz, Michel Rohmer, Jaroslaw Poznanski, Ewa Swiezewska
Summary: This study investigated the biosynthesis of dolichols and polyprenols in plant cells. It found that dolichols are derived from both the MEP and MVA pathways, while polyprenols are predominantly synthesized via the MEP pathway. The study also developed a novel methodology for analyzing isotopologues of metabolically labeled isoprenoids.
BIOCHEMICAL JOURNAL
(2023)
Article
Nutrition & Dietetics
Xiaoting Lu, Rongzhu Huang, Shuyi Li, Aiping Fang, Yuming Chen, Si Chen, Fan Wang, Xinlei Lin, Zhaoyan Liu, Huilian Zhu
Summary: The study found that higher serum betaine levels, mutations in MTHFR G1793A and A1298C genes, as well as their combined effects, are associated with a lower risk of type 2 diabetes.
Article
Pharmacology & Pharmacy
Gangadharan Leela Shyni, Kaithathara Francis Sajin, Sivasankaran Nair Mangalam, Kozhiparambil Gopalan Raghu
Summary: TM from Myristica fragrans has shown anti-obesity effects in 3T3-L1 adipocytes by reducing lipid accumulation, promoting lipolysis, and regulating genes and proteins involved in lipid metabolism. It also enhances glucose uptake and increases carnitine palmitoyltransferase levels, suggesting its potential as an anti-obesity therapeutic.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Chemistry, Inorganic & Nuclear
Wlodzmierz Buchowicz, Piotr A. . Gunka, Piotr Buchalski, Michal Piszcz, Sylwia Bus, Michal Mrozowicz, Maria Mazur, Rafal Wasilewski
Summary: In this study, a series of ansa[6]-ferrocenes were synthesized through a catalytic reaction, and the stereoselectivity of the reaction was influenced by the structure of the substrates. Single-crystal X-ray analysis showed that ansa[6]-ferrocenes were axially chiral in the solid state. Additionally, the (E) and (Z) isomers exhibited remarkable differences in solution.