期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 130, 期 2, 页码 501-510出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja072543f
关键词
-
资金
- NIGMS NIH HHS [GM059380] Funding Source: Medline
The chemical synthesis of complex glycoproteins is an ongoing challenge,in protein chemistry: We have examined the synthesis of a single glycoform of monocyte chemotactic protein-3 (MCP-3), a CC-chemokine that consists of 76 amino acids and one N-glycosylation site. A three-segment native chemical ligation strategy was employed using unprotected peptides and glycopeptide. Importantly, the synthesis required the development of methods for the generation of sialylglycopeptide-(alpha)thioesters. For the sialylglycopeptide-(alpha)thioester segment, we examined and successfully implemented approaches using Fmoc-SPPS and Boc-SPPS. To avoid use of hydrogen fluoride, the Boc approach utilized minimal side chain protection and direct thiolysis of the resin bound peptide. Using these strategies, we successfully synthesized a glycoprotein having an intact and homogeneous complex-type sialyloligosaccharide.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据