Review
Medicine, Research & Experimental
Po-Chun Hsieh, Yao-Kuang Wu, Mei-Chen Yang, Wen-Lin Su, Chan-Yen Kuo, Chou Chin-Lan
Summary: Acute lung injury (ALI) is a highly fatal disease characterized by diffuse pulmonary infiltrates. Damage-associated molecular patterns (DAMPs) play a crucial role in the initiation and progression of ALI. Proinflammatory cytokine overproduction, cell death, destruction of alveoli-endothelial barriers, and neutrophil infiltration are the main features of ALI. Alveolar macrophages (AMs), neutrophils, and epithelial cells (AECs) are important players in the pathogenesis of ALI.
Article
Multidisciplinary Sciences
Rouven Schulz, Medina Korkut-Demirbas, Gloria Colombo, Sandra Siegert
Summary: This study introduces a chemogenetic approach to study G protein-coupled receptors (GPCRs) by designing GPCR chimeras that can mimic the functions of the target receptors without knowing their immediate ligands. The researchers demonstrate the successful use of this approach in studying the beta 2-adrenergic receptor in microglia and its role in inflammation. The results show that the chimeric receptors exhibit similar responses to the endogenous receptors in terms of signaling pathways and cellular processes. This new approach allows for the investigation of cell type-specific pathways without the need for known ligands.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Huaqiao Tang, Dan Yang, Ling Zhu, Fei Shi, Gang Ye, Hongrui Guo, Huidan Deng, Ling Zhao, Zhiwen Xu, Yinglun Li
Summary: The study demonstrates that paeonol can inhibit the virulence of P.aeruginosa and decrease the inflammation response, providing potential for the development of new anti-infective drugs.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Gastroenterology & Hepatology
Ombretta Colasanti, Rani Burm, Hao-En Huang, Tobias Riedl, Jannik Traut, Nadine Gillich, Teng-Feng Li, Laura Corneillie, Suzanne Faure-Dupuy, Oliver Gruenvogel, Danijela Heide, Ji-Young Lee, Cong Si Tran, Uta Merle, Maria Chironna, Florian F. W. Vondran, Katharina Esser-Nobis, Marco Binder, Ralf Bartenschlager, Mathias Heikenwaelder, Philip Meuleman, Volker Lohmann
Summary: This study investigated the immune activation and counteraction mechanisms of hepatitis A virus (HAV) and hepatitis C virus (HCV) in vivo and in vitro. The results showed that HAV induces innate immune response in hepatocytes via the MDA5/LGP2 pathway, with limited control by proteolytic cleavage. In contrast, HCV mainly activates the Toll-like receptor 3 pathway and lacks efficient TRIF cleavage. These findings provide new insights into the immune activation and counteraction by HAV and HCV.
JOURNAL OF HEPATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ga-Yul Min, Ji-Hye Kim, Tae-In Kim, Won-Kyung Cho, Ju-Hye Yang, Jin-Yeul Ma
Summary: This study investigated the therapeutic effects of IndigoPulverata Levis extract (CHD) on atopic dermatitis (AD). The results showed that CHD reduced skin thickness, inhibited immune cell infiltration, decreased proinflammatory cytokine expression, and suppressed NF-κB activation. These findings suggest that CHD may be a potential drug candidate for AD treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Edith Reuschel, Martina Toelge, Sebastian Haeusler, Ludwig Deml, Birgit Seelbach-Goebel, Maria Emilia Solano
Summary: This study highlights the importance of cytokine responses to bacterial infections during pregnancy, particularly in the pathogenesis of preterm birth and neonatal sepsis. The results suggest that IL-8 may be a valuable diagnostic marker for timely detection of infections in preterm neonates, and that MIP-1 beta could play a role in sex-specific immunity and vulnerability to preterm birth in males.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Paulo Anastacio Purtado Pacheco, Juliana Vieira Faria, Ana Claudia Silva, Natalia Lidmar von Ranke, Robson Coutinho Silva, Carlos Rangel Rodrigues, David Rodrigues da Rocha, Robson Xavier Faria
Summary: This study evaluated the pharmacological effects of eleven N, S-acetal juglone derivatives as P2x7R inhibitors. Through in vitro and in vivo experiments, we identified one derivative with promising inhibitory activity and low toxicity. Our in silico studies suggest that the 1,4-naphthoquinone moiety might be a valuable molecular scaffold for the development of novel P2x7R antagonists, consistent with our previous research.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Immunology
Binjie Lu, Xiaolei Chen, Hua Chen, Qian Li, Hongwei Li, Yue Xu, Yuwei Li, Xiaofeng Shen, Rilei Jiang
Summary: Demethoxycurcumin (DMC) can reduce the production of inflammatory cytokines in nucleus pulposus (NP) cells induced by inflammatory radicular pain, and this effect is mediated by inhibiting the activation of MAPKs and NF-kappa B signaling pathway. Further experiments in LDH model rats confirmed the in vitro results. These studies expand our knowledge of the effect of DMC on LDH, suggesting that DMC may be a viable alternative for the treatment of LDH.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Hao Huang, Jian Wang, Yingying Fan, Ibrahim Abdel Aziz Ibrahim, Vishnu Priya Veeraraghavan
Summary: The study demonstrates the therapeutic properties of rubusoside against LPS-induced acute lung injury (ALI) in mice. Rubusoside effectively inhibits inflammatory responses and shows potential as an effective drug for treating ALI in the future.
APPLIED NANOSCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Markus Waldeck-Weiermair, Shambhu Yadav, Fotios Spyropoulos, Christina Kruger, Arvind K. Pandey, Thomas Michel
Summary: Hydrogen peroxide (H2O2) serves as a versatile cell signaling molecule at low concentrations, but can be toxic at higher concentrations by promoting oxidative stress. The differential distribution of H2O2 in cells and the varied distribution of redox-active enzymes play crucial roles in cellular functions. Chemogenetic approaches utilizing D-amino acid oxidase (DAAO) have been developed to dynamically regulate H2O2 levels in specific subcellular locations, offering insights into the physiological and pathological oxidative pathways.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Immunology
Diego Ulisse Pizzagalli, Alain Pulfer, Marcus Thelen, Rolf Krause, Santiago F. Gonzalez
Summary: This review discusses the importance of immune cell migration in inflammation and the use of intravital microscopy to visualize cell migration. The comprehensive classification of immune cell motility patterns observed in vivo and their relevance to the inflammatory process is still lacking. The review provides a definition and consensus naming for different cell actions, along with an overview of computational methods used for quantification, aiming to foster interdisciplinary research on the immune system using imaging data.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rachel Johnston, Brendan Mathias, Stephanie J. Crowley, Haley A. Schmidt, Lynn S. White, Nima Mosammaparast, Abby M. Green, Jeffrey J. Bednarski
Summary: Developing B cells generate DNA double-stranded breaks (DSBs) to assemble immunoglobulin receptor (Ig) genes necessary for the expression of a mature B cell receptor. RAG-mediated DSBs in pre-B cells activate the ATM kinase, coordinating canonical and non-canonical DDR and triggering DSB repair and B cell developmental signals. Unique properties of the RAG endonuclease regulate the distinct cellular responses to RAG DSBs in B cells.
Article
Plant Sciences
Ji Hye Kim, Jae Gwang Park, Yo Han Hong, Kon Kuk Shin, Jin Kyeong Kim, Young-Dong Kim, Ki Dong Yoon, Kyung-Hee Kim, Byong Chul Yoo, Gi-Ho Sung, Jae Youl Cho
Summary: The study found that Sb-EE from Sauropus brevipes Mull. Arg. effectively suppressed nitric oxide release and modulated inflammatory responses in various inflammation models, indicating its potential as an anti-inflammatory agent.
PHARMACEUTICAL BIOLOGY
(2021)
Article
Immunology
Sanne G. S. Verberk, Hendrik J. P. van der Zande, Jeroen Baardman, Kyra E. de Goede, Karl J. Harber, Eelco D. Keuning, Joost M. Lambooij, Frank Otto, Anna Zawistowska-Deniziak, Helga E. de Vries, Menno P. J. de Winther, Bruno Guigas, Jan Van den Bossche
Summary: Research indicates that despite enhanced inflammatory responses in vitro, deficiency of macrophage Acly does not worsen acute and chronic inflammatory responses in vivo. Myeloid-specific Acly deletion has limited impact on inflammation and overall metabolic homeostasis in inflammatory disorders like multiple sclerosis and obesity.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Physiology
Brian K. McFarlin, David W. Hill, Jakob L. Vingren, John H. Curtis, Elizabeth A. Tanner
Summary: Research found that the greatest changes in immune pathways after all-out running efforts occurred at 2 hours for subjects receiving optimized curcumin, pomegranate ellagitannins, and MSM supplementation, followed by smaller changes at 4 and 8 hours. The group receiving R + MSM showed an increase in innate immunity and a reduction in damage-associated molecular pattern signaling/inflammation, along with previously unlinked changes in immune-associated mRNA.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Immunology
Brad T. Sherman, Xiaojun Hu, Kanal Singh, Lillian Haine, Adam W. Rupert, James D. Neaton, Jens D. Lundgren, Tomozumi Imamichi, Weizhong Chang, H. Clifford Lane
Summary: The study identified 11 loci associated with three biomarker levels in PLWH, including six known in the general population and five novel loci. These findings support the hypothesis that host genetics may partly contribute to chronic inflammation in PLWH.
Article
Immunology
Hongyan Sui, Qian Chen, Tomozumi Imamichi
Summary: Human Ku70 protein functions as a cytosolic DNA sensor, translocating from the nucleus to the cytoplasm upon DNA transfection or infection with DNA virus. This translocation predominantly induces interferon lambda1 (IFN-lambda 1) through a STING-dependent signalling pathway. The cytoplasmic translocation of Ku70 is an essential and early event for its cytosolic DNA sensing, with its acetylation status influencing DNA-mediated IFN-lambda 1 induction.
Article
Biochemistry & Molecular Biology
Tomozumi Imamichi, Suranjana Goswami, Xiaojun Hu, Sylvain Laverdure, Jun Yang, Ju Qiu, Qian Chen, Brad T. Sherman, Weizhong Chang
Summary: IL-27 treatment led to the discovery of 38 novel miRNAs in differentiated macrophages, with 16 of them being differentially regulated. Overexpression of miRAB40 showed potent HIV-inhibiting and autophagy-inducing properties, partially mediated via IFN-dependent and -independent pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Suranjana Goswami, Xiaojun Hu, Qian Chen, Ju Qiu, Jun Yang, Deepak Poudyal, Brad T. Sherman, Weizhong Chang, Tomozumi Imamichi
Summary: IL-27 pretreatment promotes CD4(+) T cells into HIV-1-resistant cells by inhibiting an uncoating step, and also leads to the discovery of 15 novel microRNAs. Among them, miRTC14 shows potent anti-HIV effects in macrophages through induction of IFN-α and IFN-λ 1 production, suggesting it may serve as a potential therapeutic target for HIV and other viral infections.
JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES
(2021)
Article
Biochemistry & Molecular Biology
Xiaojun Hu, Suranjana Goswami, Ju Qiu, Qian Chen, Sylvain Laverdure, Brad T. Sherman, Tomozumi Imamichi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Tomozumi Imamichi, John G. Bernbaum, Sylvain Laverdure, Jun Yang, Qian Chen, Helene Highbarger, Ming Hao, Hongyan Sui, Robin Dewar, Weizhong Chang, H. Clifford Lane
Summary: A genome-wide association study identified 14 naturally occurring SNPs in HIV associated with virus load, with a variant containing a Met-to-Ile change in integrase found to be impaired in replication. The study also showed that GagPol assembly, virus release, and autoprocessing are regulated not only by integrase but also by RNase H.
JOURNAL OF VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Sylvain Laverdure, Ziqiu Wang, Jun Yang, Takuya Yamamoto, Tima Thomas, Toyotaka Sato, Kunio Nagashima, Tomozumi Imamichi
Summary: IL-27 has been shown to induce autophagy in macrophages, particularly when combined with huAB. The induction of autophagy by IL-27 was found to be independent of the dephosphorylation of mTOR or lipidation of LC3, suggesting a novel non-canonical pathway. Additionally, this study demonstrated that IL-27 induces autophagy during monocyte-to-macrophage differentiation in a subtype-dependent manner.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Raphael M. Oguariri, Terrence W. Brann, Joseph W. Adelsberger, Qian Chen, Suranjana Goswami, Anthony R. Mele, Tomozumi Imamichi
Summary: S100A8 and S100A9 proteins play important roles in inflammation and infection, and they have been identified as novel biomarkers for SARS-CoV-2 infection. However, their role in HIV replication is still unclear. This study found that these proteins inhibit HIV in macrophages but have no impact on T cells.
AIDS RESEARCH AND HUMAN RETROVIRUSES
(2022)
Article
Medicine, General & Internal
J. D. Lundgren, R. L. Gottlieb, U. Sandkovsky, S. M. Brown, J. Baker, A. A. Ginde, C. C. Chang, A. L. Goodman, E. S. Higgs, D. D. Murray, T. A. Murray, R. Paredes, A. N. Phillips, H. Cao, A. G. Babiker, V. J. Davey, A. C. Gelijns, V. L. Kan, M. N. Polizzotto, B. T. Thompson, H. C. Lane, J. D. Neaton
Summary: The study aimed to evaluate the efficacy and safety of bamlanivimab in COVID-19 patients, with results indicating potential benefits in patients without neutralizing antibodies at baseline. However, due to the limited sample size, further research is needed to assess the effects of other types of passive immune therapies in the same patient setting.
ANNALS OF INTERNAL MEDICINE
(2022)
Article
Virology
Jun Yang, Ming Hao, Muhammad A. Khan, Muhammad T. Rehman, Helene C. Highbarger, Qian Chen, Suranjana Goswami, Brad T. Sherman, Catherine A. Rehm, Robin L. Dewar, Weizhong Chang, Tomozumi Imamichi
Summary: The study found that a recombinant HIV-1NL4.3 with IN:M50I mutation exhibits suppression of virus release and replication incompetence. Coexisting mutations at codon 17 of IN or codon 79 of RNaseH compensate for the defective phenotype. A combination of IN:M50I and IN:V151I mutations, but not IN:M50I alone, results in a defective virus.
Review
Immunology
Hongyan Sui, Ming Hao, Weizhong Chang, Tomozumi Imamichi
Summary: Ku70, known for its role in DNA repair, has been identified as a multifunctional protein involved in various cellular processes. This review focuses on its role as a cytosolic DNA sensor, inducing interferons and proinflammatory cytokines, and discusses the unique structure of Ku70 binding with DNA and its involvement in the innate immune response pathway. Strategies to modulate Ku70-mediated innate immune response are also discussed, along with potential physiological insights based on its role in innate immunity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Brad T. Sherman, Ming Hao, Ju Qiu, Xiaoli Jiao, Michael W. Baseler, H. Clifford Lane, Tomozumi Imamichi, Weizhong Chang
Summary: DAVID, a popular bioinformatics resource system, has undergone updates in 2021, including the rebuilding of the DAVID Gene system, increasing the number of gene-term records for annotation types, adding new annotations, assigning specific subgroups, and adding a species parameter for improved user experience.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Virology
Weizhong Chang, Xiaoli Jiao, Hongyan Sui, Suranjana Goswami, Brad T. Sherman, Caroline Fromont, Juan Manuel Caravaca, Bao Tran, Tomozumi Imamichi
Summary: This study assembled and annotated the complete genome of HSV-2 strain G using PacBio long sequencing reads. It analyzed the 'alpha' sequence and identified packaging signals homologous to HSV-1, as well as determined the termini and cleavage site of the linear genome. The study also visualized four HSV-2 genome isomers using Oxford Nanopore Technology sequencing reads. Additionally, the coding sequences of strain G were compared with other HSV-2 strains and a phylogenetic analysis was conducted.
Article
Multidisciplinary Sciences
Hongyan Sui, Qian Chen, Jun Yang, Selena Srirattanapirom, Tomozumi Imamichi
Summary: Study found that manganese enhances DNA-mediated interferon production and boosts antiviral immunity, potentially offering a new approach for nucleic-acid-based treatment of infectious diseases.
Article
Virology
Tomozumi Imamichi, Qian Chen, Ming Hao, Weizhong Chang, Jun Yang
Summary: The defect in HIV(IN:M50I/V151I) virus release and autoprocessing initiation is regulated by the C-terminal domains of RNase H and integrase. Specifically, aspartic acid at codon 109 of RNase H C-terminal domain and aspartic acid at the C terminus of integrase induce the defect.