期刊
JOURNAL OF SYNCHROTRON RADIATION
卷 20, 期 -, 页码 383-385出版社
WILEY-BLACKWELL
DOI: 10.1107/S0909049513001866
关键词
SAXS; protein-protein complex; home source; chromatography; hybrid pixel X-ray detector
资金
- Motor Neurone Disease Association
- University of Liverpool
This study analyses the potential for laboratory-based size-exclusion chromatography (SEC) integrated small-angle X-ray scattering (SAXS) instrumentation to characterize protein complexes. Using a high-brilliance home source in conjunction with a hybrid pixel X-ray detector, the efficacy of SAXS data collection at pertinent protein concentrations and exposure times has been assessed. Scattering data from SOD1 and from the complex of SOD1 with its copper chaperone, using 10 min exposures, provided data quality in the range 0.03 < q < 0.25 angstrom(-1) that was sufficient to accurately assign radius of gyration, maximum dimension and molecular mass. These data demonstrate that a home source with integrated SEC-SAXS technology is feasible and would enable structural biologists studying systems containing transient protein complexes, or proteins prone to aggregation, to make advanced preparations in-house for more effective use of limited synchrotron beam time.
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