Article
Multidisciplinary Sciences
Sam Horrell, Danny Axford, Nicholas E. Devenish, Ali Ebrahim, Michael A. Hough, Darren A. Sherrell, Selina L. S. Storm, Ivo Tews, Jonathan A. R. Worrall, Robin L. Owen
Summary: The user manual for fixed target data collection at I24, Diamond Light Source provides detailed step-by-step instructions, figures, and videos to assist users in smooth data collection with serial data collection technique.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Biochemical Research Methods
Kazuya Hasegawa, Seiki Baba, Takashi Kawamura, Masaki Yamamoto, Takashi Kumasaka
Summary: Synchrotron serial crystallography (SSX) is an emerging data-collection method for micro-crystallography on synchrotron macromolecular (MX) crystallography beamlines. By examining the feasibility of the fixed-target approach and developing the humid air and glue-coating (HAG) method, it was found that rotation is effective for efficient data collection and can be applied for room-temperature data collection in micro-crystallography. Absorbed doses up to 210 kGy were found to be tolerable, and an equation was proposed to relate achievable resolution to total photon flux used for data collection.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2021)
Article
Engineering, Electrical & Electronic
V. A. Pan, L. T. Tran, Z. Pastuovic, D. Hill, J. Williams, A. Kok, M. Povoli, A. Pogossov, S. Peracchi, D. Boardman, J. Davis, S. Guatelli, M. Petasecca, M. L. F. Lerch, A. B. Rosenfeld
Summary: A new batch of microdosimeters was studied for their charge collection efficiency (CCE) and radiation hardness for various applications. The microdosimeters were characterized with different ion beams and a gamma source, and a negative pulse was observed in addition to the positive pulses generated by incident ions. It was determined that the negative pulse originated from the support wafer due to the displacement current phenomenon. Radiation hardness study showed a slight CCE deficit, but overall the microdosimeters were found suitable for space and medical applications.
IEEE TRANSACTIONS ON NUCLEAR SCIENCE
(2023)
Article
Multidisciplinary Sciences
Hanano Yamada, Vanessa S. Dias, Andrew G. Parker, Hamidou Maiga, Carina Kraupa, Marc J. B. Vreysen, Wadaka Mamai, Marc F. Schetelig, Nanwintoum S. Bimbile Somda, Jeremy Bouyer
Summary: Reproductive sterility is crucial for the success of the sterile insect technique, and the interaction between dose and dose rate has significant effects on irradiated mosquitoes. The study found that dose rate not only alters the radiation-induced effects, but also that the interaction is non-linear and may vary with dose.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Niclas U. Lundsgaard, Rebecca L. Cramp, Craig E. Franklin
Summary: The study found that UVBR exposure has beneficial physiological effects on Australian green tree frog tadpoles, contrary to general assumptions. UVBR irradiance influences the effects of UVBR dose, suggesting they do not have a proportional relationship.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Engineering, Biomedical
Frank van den Heuvel, Anna Vella, Francesca Fiorini, Mark Brooke, Mark Hill, Anderson Ryan, Tim Maughan, Amato Giaccia
Summary: A methodology for predicting tissue sparing effects in pulsed ultra-high dose rate radiation exposures is introduced and illustrated using published experiments. The proposed system quantifies the effects by formalizing the variability of oxygen levels as an oxygen dose histogram and calculating the change in DNA-damage induction based on the oxygen fixation concept. The results show that the FLASH-effect depends on the initial oxygenation level in tissue, the total dose delivered, pulse length, and pulse repetition rate.
PHYSICS IN MEDICINE AND BIOLOGY
(2022)
Article
Environmental Sciences
Thoa Nguyen Phuong, Shingo Kaneko, Shishido Koya, Hajime Ohira, Hirofumi Tsukada
Summary: This study estimated the internal and external radiation doses to trees and plants near the Fukushima Dai-ichi Nuclear Power Station, finding that the total dose rates to cedars were within the reference levels, while the rates for plants were much lower compared to grasses and herbs. The research also revealed varying internal radiation dose rates among plant species, with considerable differences observed due to the presence of specific species.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Multidisciplinary Sciences
Leonie Wenz, Robert Devon Carr, Noah Koegel, Maximilian Kotz, Matthias Kalkuhl
Summary: This study introduces the DOSE database, which contains reported economic output data from 1,661 sub-national regions across 83 countries from 1960 to 2020. The database provides the opportunity for detailed analyses of economic development at the subnational level.
Article
Materials Science, Multidisciplinary
Hyosim Kim, Jonathan G. Gigax, Osman El-Atwani, Matthew R. Chancey, Jon K. Baldwin, Yongqiang Wang, Stuart A. Maloy
Summary: The irradiation of ferritic-martensitic (F/M) HT9 and fully ferritic HT9 alloys revealed different void swelling behaviors under carbon contamination, indicating a correlation with the stability of carbides within the materials.
MATERIALS CHARACTERIZATION
(2021)
Article
Biochemistry & Molecular Biology
Min Ji Bae, Min Kook Kang, Yong Uk Kye, Jeong-Hwa Baek, Ye-Ji Sim, Hae-June Lee, Yeong-Rok Kang, Wol Soon Jo, Joong Sun Kim, Chang Geun Lee
Summary: The study revealed that the radiation dose rate has different effects on the testes of mice. Low dose rate radiation caused more severe tissue toxicity and adverse effects on sperm production compared to high dose rate radiation. This suggests that the dose rate of radiation may have differential impacts on different organs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Energy & Fuels
Sophie Duzellier, Thierry Nuns, Romain Rey, Claude Pons, Soufian Yjjou, Athina Varotsou, Carsten Baur, Christel Noemayr, Ana Gras, Ainhoa Martinez de Olcoz
Summary: This paper presents extensive irradiation test campaigns conducted on triple-junction solar cells to compare the degradation caused by different configurations of irradiation. The results support the validation of a new tool developed to optimize the accuracy of solar cell degradation prediction. The study addresses the equivalence of degradation induced by electrons, protons, and combined electrons and protons, taking into account local shielding.
PROGRESS IN PHOTOVOLTAICS
(2023)
Article
Materials Science, Multidisciplinary
D. Cosic, G. Provatas, M. Jaksic, D. Begusic
Summary: The charge collection efficiency of a diamond detector was studied at different temperatures using ions of different energies. The efficiency decreased with decreasing temperature, showing a plateau at room temperature to 145 K followed by a sharp decrease in the range of 65 K < T < 145 K. Below 65 K, the efficiency reached another plateau with small variations. The decrease in collected charge was observed for all ions, but the degree of decrease varied. The reduction in efficiency is consistent with previous results and is associated with changes in exciton lifetime in diamond.
DIAMOND AND RELATED MATERIALS
(2022)
Article
Biology
Xiaoman Duan, Xiao Fan Ding, Naitao Li, Fang-Xiang Wu, Xiongbiao Chen, Ning Zhu
Summary: This study presents a novel low-dose imaging strategy called Sparse2Noise, which combines data from sparse-view CT scan and full-view CT scan using a convolutional neural network. Sparse2Noise effectively reduces noise and artifacts while maintaining high image quality.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Multidisciplinary Sciences
O. El Atwani, H. T. Vo, M. A. Tunes, C. Lee, A. Alvarado, N. Krienke, J. D. Poplawsky, A. A. Kohnert, J. Gigax, W. -Y Chen, M. Li, Y. Q. Wang, J. S. Wrobel, D. Nguyen-Manh, J. K. S. Baldwin, O. U. Tukac, E. Aydogan, S. Fensin, E. Martinez
Summary: The authors develop an irradiation resistant, thermally stable, and strong refractory high entropy alloy (RHEA) for nuclear application through a combination of simulation and experiments. This research is of great significance in the quest for new materials that can withstand severe irradiation and mechanical extremes for advanced applications.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Amr Ghaleb, Lucia Roa, Natalia Marchenko
Summary: The biological consequences of low-dose radiation (LDR) in breast cancer are unclear, while high-dose radiation (HDR) promotes DNA repair and cell cycle arrest. Mutant p53 promotes mammary tumorigenesis following LDR, but has negligible effects on tumors carrying the wild-type p53 allele.
Article
Instruments & Instrumentation
Isabelle Martiel, Aldo Mozzanica, Nadia L. Opara, Ezequiel Panepucci, Filip Leonarski, Sophie Redford, Istvan Mohacsi, Vitaliy Guzenko, Dmitry Ozerov, Celestino Padeste, Bernd Schmitt, Bill Pedrini, Meitian Wang
JOURNAL OF SYNCHROTRON RADIATION
(2020)
Article
Chemistry, Physical
Filip Leonarski, Aldo Mozzanica, Martin Bruckner, Carlos Lopez-Cuenca, Sophie Redford, Leonardo Sala, Andrej Babic, Heinrich Billich, Oliver Bunk, Bernd Schmitt, Meitian Wang
STRUCTURAL DYNAMICS-US
(2020)
Article
Multidisciplinary Sciences
Petr Skopintsev, David Ehrenberg, Tobias Weinert, Daniel James, Rajiv K. Kar, Philip J. M. Johnson, Dmitry Ozerov, Antonia Furrer, Isabelle Martiel, Florian Dworkowski, Karol Nass, Gregor Knopp, Claudio Cirelli, Christopher Arrell, Dardan Gashi, Sandra Mous, Maximilian Wranik, Thomas Gruhl, Demet Kekilli, Steffen Bruenle, Xavier Deupi, Gebhard F. X. Schertler, Roger M. Benoit, Valerie Panneels, Przemyslaw Nogly, Igor Schapiro, Christopher Milne, Joachim Heberle, Joerg Standfuss
Article
Chemistry, Multidisciplinary
Xiaopan Gao, Kaixiang Zhu, Justyna Wojdyla, Pu Chen, Bo Qin, Ziheng Li, Meitian Wang, Sheng Cui
Article
Biochemical Research Methods
Greta M. Assmann, Meitian Wang, Kay Diederichs
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2020)
Article
Biology
Xia Yu, Xiaopan Gao, Kaixiang Zhu, Han Yin, Xujian Mao, Justyna Aleksandra Wojdyla, Bo Qin, Hairong Huang, Meitian Wang, Yi-Cheng Sun, Sheng Cui
COMMUNICATIONS BIOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Karol Nass, Robert Cheng, Laura Vera, Aldo Mozzanica, Sophie Redford, Dmitry Ozerov, Shibom Basu, Daniel James, Gregor Knopp, Claudio Cirelli, Isabelle Martiel, Cecilia Casadei, Tobias Weinert, Przemyslaw Nogly, Petr Skopintsev, Ivan Usov, Filip Leonarski, Tian Geng, Mathieu Rappas, Andrew S. Dore, Robert Cooke, Shahrooz Nasrollahi Shirazi, Florian Dworkowski, May Sharpe, Natacha Olieric, Camila Bacellar, Rok Bohinc, Michel O. Steinmetz, Gebhard Schertler, Rafael Abela, Luc Patthey, Bernd Schmitt, Michael Hennig, Joerg Standfuss, Meitian Wang, Christopher J. Milne
Article
Chemistry, Multidisciplinary
Isabelle Martiel, Chia-Ying Huang, Pablo Villanueva-Perez, Ezequiel Panepucci, Shibom Basu, Martin Caffrey, Bill Pedrini, Oliver Bunk, Marco Stampanoni, Meitian Wang
Article
Multidisciplinary Sciences
Xiaopan Gao, Kaixiang Zhu, Bo Qin, Vincent Olieric, Meitian Wang, Sheng Cui
Summary: Orf9b, a unique accessory protein of SARS-CoV-2 and SARS-CoV, interacts with TOM70 to potentially interfere with immunity and suppress interferon responses by inhibiting the Hsp90/TOM70 interaction. The specific mechanisms of these interactions shed light on the pathogenesis of these coronaviruses.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Karol Nass, Camila Bacellar, Claudio Cirelli, Florian Dworkowski, Yaroslav Gevorkov, Daniel James, Philip J. M. Johnson, Demet Kekilli, Gregor Knopp, Isabelle Martiel, Dmitry Ozerov, Alexandra Tolstikova, Laura Vera, Tobias Weinert, Oleksandr Yefanov, Joerg Standfuss, Sven Reiche, Christopher J. Milne
Summary: This study demonstrates the use of large-bandwidth X-ray pulses at Swiss X-ray free-electron laser in serial femtosecond crystallography (SFX) to improve the partiality of Bragg spots and reduce sample consumption while maintaining data quality. By utilizing sensitive data-quality indicators, such as anomalous signals and de novo phasing results from microcrystals, the benefits of using pink X-ray pulses to obtain accurate structure-factor amplitudes were quantified.
Article
Biochemical Research Methods
Jakub W. Kaminski, Laura Vera, Dennis P. Stegmann, Jonatan Vering, Deniz Eris, Kate M. L. Smith, Chia Ying Huang, Nathalie Meier, Julia Steuber, Meitian Wang, Guenter Fritz, Justyna A. Wojdyla, May E. Sharpe
Summary: Fragment-based drug discovery has been proven to be an effective and efficient method for identifying new chemical scaffolds. X-ray crystallography can be used to validate and develop identified fragments, and recent technological advancements have enabled the development of dedicated platforms for FBDD using X-ray crystallography.
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sandra Mous, Guillaume Gotthard, David Ehrenberg, Saumik Sen, Tobias Weinert, Philip J. M. Johnson, Daniel James, Karol Nass, Antonia Furrer, Demet Kekilli, Pikyee Ma, Steffen Bruenle, Cecilia Maria Casadei, Isabelle Martiel, Florian Dworkowski, Dardan Gashi, Petr Skopintsev, Maximilian Wranik, Gregor Knopp, Ezequiel Panepucci, Valerie Panneels, Claudio Cirelli, Dmitry Ozerov, Gebhard F. X. Schertler, Meitian Wang, Chris Milne, Joerg Standfuss, Igor Schapiro, Joachim Heberle, Przemyslaw Nogly
Summary: By combining time-resolved crystallography, spectroscopy, and multiscale simulations, we have elucidated the molecular mechanism and structural dynamics of a chloride-pumping rhodopsin throughout the transport cycle. We identified transient anion-binding sites, provided evidence for the use of light energy in the pumping mechanism, and discovered molecular gates that ensure unidirectional transport. These findings reveal key mechanistic features that enable finely controlled chloride transport across the cell membrane in this light-powered chloride ion pump.
Article
Chemistry, Multidisciplinary
Andrea Cellini, Madan Kumar Shankar, Weixiao Yuan Wahlgren, Amke Nimmrich, Antonia Furrer, Daniel James, Maximilian Wranik, Sylvain Aumonier, Emma Beale, Florian Dworkowski, Jorg Standfuss, Tobias Weinert, Sebastian Westenhoff
Summary: This study presents the structure of a photoactivated (6-4) photolyase in its radical pair state, captured using serial crystallography. It explains how a conserved asparagine stabilizes the semiquinone FAD chromophore through hydrogen bonding, and how rearrangement of amino acids around the final tryptophan radical opens it up to the solvent. These findings highlight the importance of the protein environment in stabilizing the radical pair state for the function of (6-4) photolyases and cryptochromes.
CHEMICAL COMMUNICATIONS
(2022)
Meeting Abstract
Chemistry, Multidisciplinary
Karol Nass, Camila Bacellar, Claudio Cirelli, Florian Dworkowski, Yaroslav Gevorkov, Daniel James, Philip J. M. Johnson, Demet Kekilli, Gregor Knopp, Isabelle Martiel, Dmitry Ozerov, Alexandra Tolstikova, Laura Vera, Tobias Weinert, Oleksandr Yefanov, Jorg Standfuss, Sven Reiche, Christopher J. Milne
ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES
(2021)
Article
Chemistry, Inorganic & Nuclear
Marina Lucic, Amanda K. Chaplin, Tadeo Moreno-Chicano, Florian S. N. Dworkowski, Michael T. Wilson, Dimitri A. Svistunenko, Michael A. Hough, Jonathan A. R. Worrall
DALTON TRANSACTIONS
(2020)