Article
Veterinary Sciences
Francesca La Cava, Alberto Fringuello Mingo, Sonia Colombo Serra, Aldo Di Vito, Claudia Cabella, Paolo Oliva, Alessia Cordaro, Chiara Brioschi, Enzo Terreno, Luigi Miragoli
Summary: Breast cancer is the most common cancer among women worldwide, and CE-MRI is recommended as supplemental screening for high-risk women. This study developed a refined method to orthotopically induce breast cancer in mice and used CE-MRI to characterize the evolution of the tumoral lesion.
LABORATORY ANIMALS
(2021)
Article
Immunology
Mihaly Balogh, Jixiang Zhang, Caitlyn M. Gaffney, Neha Kalakuntla, Nicholas T. Nguyen, Ronnie T. Trinh, Clarissa Aguilar, Hoang Vu Pham, Bojana Milutinovic, James M. Nichols, Rajasekaran Mahalingam, Andrew J. Shepherd
Summary: This study demonstrates that colon cancer is associated with latent sensory neuron dysfunction and implicates cytokine/chemokine signaling in this process. These findings may have implications for determining risk factors and treatment responsiveness related to neuropathy in colorectal cancer.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Immunology
Jin Wang, Xingchen Liu, Junsong Ji, Jianhua Luo, Yuanyu Zhao, Xiaonan Zhou, Jianming Zheng, Meng Guo, Yanfang Liu
Summary: This study describes the construction of a novel orthotopic PDAC model through implantation of tissue slices and discusses the resistance to immunotherapy from the perspective of a PDAC microenvironment. Results show that elimination of MDSCs by NKG2D CAR can rescue impaired CAR-T cell activity.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Tiziano Lottini, Matteo Buonamici, Claudia Duranti, Annarosa Arcangeli
Summary: Pancreatic cancer is one of the deadliest cancer types globally due to its high resistance and malignant behavior. Developing mouse models that better mimic in vivo characteristics of PCa is critical for testing new drugs. Generating an orthotopic xenograft of PCa by ultrasound-guided injection of human pancreatic tumor cells provides reliable evidence of in vivo engraftment and tumor development.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Multidisciplinary Sciences
Navid Mohammad Mirzaei, Wenrui Hao, Leili Shahriyari
Summary: The complex network of cell and molecule interactions in the tumor microenvironment creates a diverse ecosystem. The proximity of cells and molecules to their activators and inhibitors is crucial for tumor progression. Using a combination of partial differential equations and linear elasticity, we investigate the impact of spatial interactions on the tumor microenvironment. Our findings reveal interesting patterns of cell and cytokine distribution influenced by macrophages. We also observe the recruitment and suppression of cytotoxic T cells at macrophage sites. Additionally, anti-tumor macrophages reorganize patterns in favor of a more spatially restricted cancer and necrotic core. Sensitivity analysis suggests that macrophages play a key role in controlling cancer cell population and spatial arrangement in the tumor microenvironment.
Article
Oncology
Hong-Tao Hu, Zhe Wang, Myung Ji Kim, Lu-Shang Jiang, Shi-Jun Xu, Jaeyun Jung, Eunji Lee, Jung-Hoon Park, Nader Bakheet, Sung Hwan Yoon, Kun Yung Kim, Ho-Young Song, Suhwan Chang
Summary: The study showed that using tissue adhesive to establish a colon cancer model resulted in increased graft success, decreased mortality, larger tumor size, and shorter operation time compared to the conventional surgical method. Additionally, the tissue adhesive group had similar tumor structure, immunohistochemistry staining, and most tumor markers as the suture group, with alterations in apoptosis and proliferation markers observed.
CANCER RESEARCH AND TREATMENT
(2021)
Article
Mathematical & Computational Biology
Ernesto A. B. F. Lima, Patrick N. Song, Kirsten Reeves, Benjamin Larimer, Anna G. Sorace, Thomas E. Yankeelov
Summary: The study aims to develop a mathematical model to capture the interaction between evofosfamide, immunotherapy, and the hypoxic landscape of tumors. The results show that evofosfamide enhances immune response and sensitizes hypoxic tumors to immunotherapy. However, the model fails to accurately predict the response of normoxic tumors to combination treatment with evofosfamide and immunotherapy.
MATHEMATICAL BIOSCIENCES AND ENGINEERING
(2023)
Article
Biology
Jacqui A. McGovern, Nathalie Bock, Abbas Shafiee, Laure C. Martine, Ferdinand Wagner, Jeremy G. Baldwin, Marietta Landgraf, Christoph A. Lahr, Christoph Meinert, Elizabeth D. Williams, Pamela M. Pollock, Jim Denham, Pamela J. Russell, Gail P. Risbridger, Judith A. Clements, Daniela Loessner, Boris M. Holzapfel, Dietmar W. Hutmacher
Summary: In this study, pre-seeding scaffolds with human osteoblasts increased the human cellular and extracellular matrix content of bone constructs, while the humanized prostate TME showed a trend to decrease metastasis of PC-3 cells to tissue-engineered bone. On the other hand, the humanized TME enhanced LNCaP tumor growth and metastasis to both humanized and murine bone. This emphasizes the importance of the TME in prostate cancer metastasis to bone.
COMMUNICATIONS BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Amal Senevirathne, Ram Prasad Aganja, Chamith Hewawaduge, John Hwa Lee
Summary: This study investigates the anticancer properties of SLURP1, an alpha 7-nAChR antagonist, and its role as an inflammation modulator on human colorectal cancer cell lines. The results demonstrate that SLURP1 can inhibit cell proliferation and impact cell cycle regulation and inflammatory responses. The study also confirms the potential cross-species anticancer effects of human SLURP1. Further investigations are needed to fully understand its mechanisms.
Article
Oncology
Weiyong Liu, Yunkai Zhu, Lei Ye, Yajuan Zhu, Yuhao Wang
Summary: This study successfully established an orthotopic LNCaP xenograft Balb/c athymic nude mouse model that mimics the pathophysiological process of prostate cancer in humans. Through ultrasound imaging and microscopic observation, we found that this model has good tumor characteristics and can be used for studying the angiogenesis process in prostate cancer and evaluating the clinical efficacy of anti-angiogenic therapies.
Article
Pharmacology & Pharmacy
Yunjian Li, Yupei Su, Hao Pan, Wenbin Deng, Jiahui Wang, Dandan Liu, Weisan Pan
Summary: A novel oral drug delivery system with colon localization and tumor targeting functions was designed for orthotopic colon cancer chemotherapy and photothermal combinational therapy. The system utilized nanoparticles as carriers for drug delivery, and demonstrated good photothermal effect and drug release behavior. In vivo experiments showed that this system had prolonged drug retention and exhibited satisfactory therapeutic effects.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Microbiology
Jessica Queen, Jada C. Domingue, James Robert White, Courtney Stevens, Barath Udayasuryan, Tam T. D. Nguyen, Shaoguang Wu, Hua Ding, Hongni Fan, Madison McMann, Alina Corona, Tatianna C. Larman, Scott S. Verbridge, Franck Housseau, Daniel J. Slade, Julia L. Drewes, Cynthia L. Sears
Summary: This study aimed to establish a stable murine model for the colonization of Fusobacterium nucleatum and to understand its inflammatory potential and virulence genes. The results showed that F. nucleatum subspecies did not induce tumorigenesis in mice and the level of inflammation varied among different subspecies. No distinct virulence genes associated with human colorectal cancer strains were identified. The study suggests that the difference in biology between humans and mice, as well as a synergistic role of F. nucleatum with other bacteria, may contribute to the lack of tumorigenesis in the model.
Article
Microbiology
Jessica Queen, Jada C. Domingue, James Robert White, Courtney Stevens, Barath Udayasuryan, Tam T. D. Nguyen, Shaoguang Wu, Hua Ding, Hongni Fan, Madison McMann, Alina Corona, Tatianna C. Larman, Scott S. Verbridge, Franck Housseau, Daniel J. Slade, Julia L. Drewes, Cynthia L. Sears
Summary: This study investigated the inflammatory and tumorigenic potential of several clinical isolates of F. nucleatum using multiple murine models, in vitro assays with a human colon cancer cell line, and whole-genome sequencing analysis. The results showed that F. nucleatum subspecies did not induce tumorigenesis in murine models, but did induce greater cytokine responses in a human colorectal cell line.
Article
Anatomy & Morphology
Kentaro Miyake, Qinghong Han, Takashi Murakami, Tasuku Kiyuna, Kei Kawaguchi, Kentaro Igarashi, Thinzar M. Lwin, Masuyo Miyake, Jun Yamamoto, Michael Bouvet, Itaru Endo, Robert M. Hoffman
Summary: The efficacy of recombinant methioninase (rMETase) on a mouse model of colorectal cancer liver metastasis was investigated in this study. The results showed that rMETase significantly inhibited the increase of liver metastasis. Therefore, rMETase may have future potential as a therapy for colorectal cancer liver metastasis.
Article
Surgery
Keegan Guidolin, Deanna Ng, Sami Chadi, Fayez A. Quereshy
Summary: This study aimed to compare perioperative outcomes of different surgical approaches in patients with T4aN2 colon cancer, and evaluate the influence of conversion on perioperative outcomes. The results showed that planned open surgery conferred the highest risk of complications, while surgical approach was not associated with mortality. These findings challenge the notion that conversion is associated with the worst perioperative outcomes and suggest that a minimally invasive approach should be considered for patients with locally advanced colon cancer.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2022)
Editorial Material
Surgery
Meghal Shah, Tejas S. Sathe, Sukriti Bansal, Anai N. Kothari, Sophie Dream
JOURNAL OF SURGICAL RESEARCH
(2024)
Letter
Surgery
Zafer Turkyilmaz, Ramazan Karabulut, Kaan Sonmez
JOURNAL OF SURGICAL RESEARCH
(2024)
Letter
Surgery
Priyanka Jadhav, Gerald Gollin
JOURNAL OF SURGICAL RESEARCH
(2024)
Review
Surgery
Kheira Hireche, Ludovic Canaud, Pierre Antoine Peyron, Linda Sakhri, Isabelle Serres, Sanaa Kamel, Youcef Lounes, Thomas Gandet, Pierre Alric
Summary: This study evaluated the elastic properties of commonly used vascular substitutes for pulmonary artery replacement and compared their compliance and stiffness indexes to human pulmonary artery. The results showed that allogenic arterial grafts appeared to be the most suitable vascular substitutes in terms of compliance and stiffness for PA replacement.
JOURNAL OF SURGICAL RESEARCH
(2024)
Article
Surgery
Margaret Siu, Aixa Perez Coulter, Heather M. Grant, Reginald Alouidor, Michael Tirabassi
Summary: There is no significant difference in adverse respiratory events between intubated, critically ill patients requiring operative intervention who are kept NPO for 6 hours or longer compared to those kept NPO for less than 6 hours. Patients commonly experience periods of fasting much longer than the recommended 6-hour period by the American Society of Anesthesiologists.
JOURNAL OF SURGICAL RESEARCH
(2024)
Article
Surgery
Whitney Elks, Allison G. McNickle, Matthew Kelecy, Kavita Batra, Shirley Wong, Shawn Wang, Lisa Angotti, Deborah A. Kuhls, Charles St Hill, Syed F. Saquib, Paul J. Chestovich, Douglas R. Fraser
Summary: This study aimed to compare the effects of early and late enteral feeding after PEG placement on achieving nutritional therapy goals and adverse outcomes. The results showed that patients with early initiation of feeds achieved a higher percentage of goals on day 0 without an increased rate of adverse events.
JOURNAL OF SURGICAL RESEARCH
(2024)
Article
Surgery
Manisha B. Bhatia, Cassandra M. Anderson, Abdiwahab N. Hussein, Brian Opondo, Nereah Aruwa, Otieno Okumu, Sarah G. Fisher, Tasha Sparks Joplin, JoAnna L. Hunter-Squires, Brian W. Gray, Peter W. Saula
Summary: This study aimed to understand postoperative pediatric nutrition practices in Kenya and the United States. The results showed that in the United States, patients initiated enteral nutrition earlier and had shorter hospital stays. However, in Kenya, patients initiated enteral nutrition earlier but had no significant difference in hospital stays.
JOURNAL OF SURGICAL RESEARCH
(2024)
Article
Surgery
David P. Stonko, Joseph Edwards, Hossam Abdou, Rebecca Treffalls, Patrick Walker, Jonathan J. Morrison
Summary: Raising mean arterial pressure (MAP) >90 mmHg with norepinephrine can increase gastroduodenal artery (GDA) flow and delay bowel ischemia.
JOURNAL OF SURGICAL RESEARCH
(2024)
Article
Surgery
David R. Mann, Kathryn E. Engelhardt, Barry C. Gibney, Macelyn E. Batten, Eric C. Klipsch, Rupak Mukherjee, Ian C. Bostock
Summary: Pathologic upstaging is associated with decreased overall survival in cT1b esophageal cancer. Esophagectomy has better survival outcomes compared to endoscopic local tumor excision. Neoadjuvant chemoradiation therapy does not improve overall survival in cT1b lesions.
JOURNAL OF SURGICAL RESEARCH
(2024)
Article
Surgery
Ross Mudgway, Zachary Tran, Juan C. Quispe Espiritu, Woo Bin Bong, Hayden Schultz, Vamsi Vemireddy, Aarthy Kannappan, Marcos Michelotti, Kaushik Mukherjee, Jeffrey Quigley, Keith Scharf, Daniel Srikureja, Sharon S. Lum, Esther Wu
Summary: Comparison of medium-term outcomes between robotic-assisted cholecystectomy (RC) and laparoscopic cholecystectomy (LC) using validated quality of life (QoL) and pain assessments did not show significant differences.
JOURNAL OF SURGICAL RESEARCH
(2024)
Article
Surgery
Ningjie Chen, Haitao Wang, Yang Shao, Jincun Yang, Guodong Song
Summary: This study aimed to compare the therapeutic effects of activated platelet-rich plasma (PRP) prepared from elderly individuals and young adults in treating pressure ulcers (PUs). The results showed that PRP from young adults had higher platelet concentrations and greater production of growth factors, leading to better wound healing.
JOURNAL OF SURGICAL RESEARCH
(2024)
Article
Surgery
Brendin R. Beaulieu-Jones, Margaret T. Berrigan, Kortney A. Robinson, Jayson S. Marwaha, Tara S. Kent, Gabriel A. Brat
Summary: Introduction: Prescription opioids, including those prescribed after surgery, have greatly contributed to the US opioid epidemic. Educating opioid prescribers is a crucial component of ensuring the safe use of opioids among surgical patients. This study implemented an annual education curriculum for new surgical prescribers, resulting in significant improvements in knowledge and comfort levels. However, there remains a persistent knowledge and comfort gap among these prescribers.
JOURNAL OF SURGICAL RESEARCH
(2024)
Article
Surgery
Sneha G. Bhat, Madhuri Nagaraj, Courtney Balentine, Timothy Hogan, Jennie Meier, Hillary Prince, Kareem Abdelfattah, Herbert Zeh, Benjamin Levi
Summary: This pilot study examined the effects of a structured mental fitness program on academic surgeons and found significant improvement in Positive Intelligence (PQ) scores, as well as increased connectedness and shared language among participants. However, there were no significant changes in sleep, well-being, or teaching evaluations.
JOURNAL OF SURGICAL RESEARCH
(2024)
Article
Surgery
Kristin E. Cox, Michael A. Turner, Siamak Amirfakhri, Thinzar M. Lavin, Mojgan Hosseini, Pradipta Ghosh, Marygorret Obonyo, Takashi Murakami, Robert M. Hoffman, Paul J. Yazaki, Michael Bouvet
Summary: This study demonstrates the potential of using humanized anti-carcinoembryonic antigen (CEA) antibodies conjugated with near-infrared dyes to specifically label gastric cancers in mouse models. Orthotopic models showed bright and specific labeling with more than ten times higher tumor-to-background ratios compared to the control. This tumor-specific fluorescent antibody has promising potential as a clinical tool for improving visualization of gastric cancer margins during surgical resection.
JOURNAL OF SURGICAL RESEARCH
(2024)
Review
Surgery
Sarah Maki, Melissa Leon, Emily Glenn, Tiffany Tanner, Crystal Krause
Summary: This scoping review analyzed the literature on the use of broadband personality tests in the bariatric surgical population to optimize weight loss outcomes. The study found significant associations between personality scales and weight loss, but inconsistent reporting of outcome measures made it challenging to draw concrete conclusions. The American Society for Metabolic and Bariatric Surgery recommends standardization of outcome reporting to improve the reliability of predicting weight loss outcomes.
JOURNAL OF SURGICAL RESEARCH
(2024)