Article
Multidisciplinary Sciences
Leire Torices, Caroline Nunes-Xavier, Jose I. Lopez, Rafael Pulido
Summary: PTEN is a tumor suppressor gene frequently mutated in human tumors, and its mutations are the diagnostic marker for PTEN Hamartoma Tumor Syndrome (PHTS), a disorder characterized by multiple hamartomas, cancer predisposition, and neurodevelopmental alterations. This study generated and characterized anti-PTEN monoclonal antibodies recognizing the PTEN C2-domain, which can be used to study the expression and function of PTEN isoforms and mutations associated with disease. These antibodies are suitable for investigating the pathogenicity of PTEN C-terminal truncations that have lost the PTEN C-terminal epitopes but retain stability and function.
Article
Biochemistry & Molecular Biology
Rohit Karn, Isaac Arnold Emerson
Summary: This study focused on the most frequently occurring PTEN mutation in breast cancer and found that frameshift mutations are the most damaging to the C2 domain. Substitution mutations D92H and R130Q were found to interfere with the dephosphorylation process of normal PTEN. The C2 domain is more frequently mutated and shows greater fluctuations in amino acid residues compared to other regions. The study provides a basis for selecting the frequently mutated C2 domain as a potential therapeutic marker.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Laura H. John, Fiona B. Naughton, Mark S. P. Sansom, Andreas Haahr Larsen
Summary: Phosphatase and tensin homologue (PTEN) and SH2-containing inositol 5 '-phosphatase 2 (SHIP2) are structurally and functionally similar, but their C2 domains have different roles in membrane interactions. The C2 domain of PTEN strongly interacts with anionic lipids and contributes to membrane recruitment, while the C2 domain of SHIP2 has weaker binding affinity for anionic membranes. The C2 domain in SHIP2 enhances the catalytic activity of the phosphatase domain through allosteric interdomain changes.
Review
Oncology
Anne Liu, Yanyu Zhu, Weiping Chen, Glenn Merlino, Yanlin Yu
Summary: PTEN is a critical tumor suppressor gene with multifunctional phosphatase activity, and its protein-phosphatase activity plays a crucial role in tumor progression, although its mechanism is not fully understood.
Article
Biotechnology & Applied Microbiology
Haijun Kang, Dongmei Huang, Heng Li, Xuejun Deng, Siyuan Liu, Wenjun Gou, Linglin Liu, Yuyan Qiu, Xu Yang
Summary: This study found that the long non-coding RNA lncNALT plays an important role in the pathogenesis of hypertensive retinopathy. Knockdown of lncNALT enhances the viability, proliferation, migration, and invasion of human retinal microvascular endothelial cells (RMECs) via the PTEN/PI3K/AKT pathway.
Review
Biochemistry & Molecular Biology
Danai Maria Kotzampasi, Kyriaki Premeti, Alexandra Papafotika, Vasiliki Syropoulou, Savvas Christoforidis, Zoe Cournia, George Leondaritis
Summary: This article investigates the interplay between PI3Ka and PTEN in cellular signaling and membrane trafficking, discusses the impact of their structure and catalytic activity on signaling, and explores the potential for pharmacological targeting of these proteins.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Anatomy & Morphology
Yongbo Hu, Yiming He, Wei Liu, Shun Yu, Yabing Wei, Shaohua Bai, Yu Su, Bin Xiao
Summary: SIAH2 acts as an oncogene in colorectal cancer, promoting tumor proliferation, migration, and invasion. It activates the PI3K/AKT signaling pathway and is regulated by PTEN loss.
Article
Oncology
Fabiana Conciatori, Erica Salvati, Ludovica Ciuffreda, Senji Shirasawa, Italia Falcone, Francesco Cognetti, Gianluigi Ferretti, Massimo Zeuli, Donatella Del Bufalo, Chiara Bazzichetto, Michele Milella
Summary: Soluble factors produced by stromal fibroblasts can influence the sensitivity of tumor cells to molecularly targeted drugs. This influence depends on the genetic mutations and PTEN protein status of the tumor cells.
FRONTIERS IN ONCOLOGY
(2022)
Article
Multidisciplinary Sciences
Feng Zhi, Bowen Li, Chuanxia Zhang, Fan Xia, Rong Wang, Weihong Xie, Sihui Cai, Dawei Zhang, Ren Kong, Yiqiao Hu, Yilin Yang, Ya Peng, Jun Cui
Summary: NLRP6 enhances the PI3K/AKT pathway by binding and degrading p85 alpha, promoting tumorigenesis. The interaction between NLRP6 and p85 alpha offers a potential therapeutic target for tumor treatment.
NATURE COMMUNICATIONS
(2023)
Article
Oncology
Wei-Pang Chung, Wei-Lun Huang, Chun-Hui Lee, Hui-Ping Hsu, Wan-Ling Huang, You-Yu Liu, Wu-Chou Su
Summary: The activation of the PI3K signaling pathway is associated with the development and resistance of breast cancer. Mutations in PIK3CA and loss of PTEN occur in all subtypes of breast cancer. The combination of PI3K inhibitors and trastuzumab shows potential in treating HER2-positive breast cancer, but its effectiveness in HER2-positive breast cancer with PIK3CA mutations and/or PTEN loss is still unclear. In this study, a novel alpha isoform-specific PI3K inhibitor was shown to effectively target breast cancer cells with PIK3CA mutations or PTEN deficiency when combined with trastuzumab. The combination therapy was found to be ineffective in breast cancer cells without PI3K pathway alterations, indicating a biomarker-specific treatment. In vivo testing in a mouse model confirmed the efficacy of the combination therapy in reducing tumor volume. This cytotoxic chemotherapy-free regimen, as a biomarker-driven strategy, warrants further investigation for the treatment of HER2-positive breast cancer.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Multidisciplinary Sciences
Yanlin Yu, Meng Dai, Liping Huang, Weiping Chen, Ellen Yu, Arnulfo Mendoza, Helen Michael, Chand Khanna, Marcus Bosenberg, Martin McMahon, Glenn Merlino
Summary: This study found that PTEN expression negatively correlates with the metastatic progression of melanoma. Wildtype PTEN expression inhibits melanoma cell invasiveness and metastasis in a dose-dependent manner, which specifically requires PTEN protein phosphatase activity. PTEN phosphatase activity inhibits metastasis by regulating the Entpd5/IGF1R pathway through ATF6.
Article
Agriculture, Dairy & Animal Science
Soo-Hyeon Kim, Byung-Joon Seung, Seung-Hee Cho, Ha-Young Lim, Min-Kyung Bae, Jung-Hyang Sur
Summary: The frequency of PIK3CA H1047R mutation in canine mammary tumors is 14.3%, leading to dysregulation of downstream molecules Akt2, p-Akt, and PTEN, with the exception of a higher level of PTEN in mutated tumors. No significant correlation was found between PIK3CA H1047R mutation and expression of downstream molecules, suggesting the dysregulation of components in the PI3K/Akt/PTEN pathway is a feature in canine mammary tumors distinct from the mutation.
Article
Emergency Medicine
Dongming Lv, Zhongye Xu, Pu Cheng, Zhicheng Hu, Yunxian Dong, Yanchao Rong, Hailin Xu, Zhiyong Wang, Xiaoling Cao, Wuguo Deng, Bing Tang
Summary: This study demonstrated the involvement of the SNO-DJ-1/SNO-PTEN/PI3K/AKT/mTOR axis in keloid formation, suggesting that SNO of DJ-1 could be a novel therapeutic target for keloid treatment.
Article
Biotechnology & Applied Microbiology
Qin Zhu, Xian Zhang, Hong-Yan Zai, Wei Jiang, Ke-Jing Zhang, Yu-Qiong He, Yu Hu
Summary: The study suggests that circSLC8A1 plays a role in breast cancer by regulating the miR-671 and PTEN/PI3k/Akt signaling pathways, leading to inhibition of tumor growth and metastasis.
Review
Cell Biology
Santiago Vidal, Yanis Hichem Bouzaher, Ahmed El Motiam, Rocio Seoane, Carmen Rivas
Summary: The PI3K/AKT pathway, regulated by SUMOylation, plays a crucial role in various physiological processes and may be involved in human diseases.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Virology
Robert A. Dick, Marilia Barros, Danni Jin, Mathias Loesche, Volker M. Vogt
JOURNAL OF VIROLOGY
(2016)
Article
Biochemical Research Methods
Hirsh Nanda, Frank Heinrich, Mathias Loesche
Article
Biochemistry & Molecular Biology
Frank Heinrich, Srinivas Chakravarthy, Hirsh Nanda, Antonella Papa, Pier Paolo Pandolfi, Alonzo H. Ross, Rakesh K. Harishchandra, Arne Gericke, Mathias Loesche
Article
Biochemistry & Molecular Biology
Lauren O'Neil, Kathryn Andenoro, Isabella Pagano, Laura Carroll, Leah Langer, Zachary Dell, Davina Perera, Bradley W. Treece, Frank Heinrich, Mathias Losche, John F. Nagle, Stephanie Tristram-Nagle
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2016)
Article
Virology
Marilia Barros, Frank Heinrich, Siddhartha A. K. Datta, Alan Rein, Ioannis Karageorgos, Hirsh Nanda, Mathias Loesche
JOURNAL OF VIROLOGY
(2016)
Article
Biology
Yang Shen, Marilia Barros, Tarek Vennemann, D. Travis Ggallagher, Yizhou Yin, Sara B. Linden, Ryan D. Heselpoth, Dennis J. Spencer, David M. Donovan, John Moult, Vincent A. Fischetti, Frank Heinrich, Mathias Losche, Daniel C. Nelson
Article
Biochemistry & Molecular Biology
Kerstin Zimmermann, Rebecca Eells, Frank Heinrich, Stefanie Rintoul, Brian Josey, Prabhanshu Shekhar, Mathias Losche, Lawrence J. Stern
JOURNAL OF BIOLOGICAL CHEMISTRY
(2017)
Article
Biophysics
Rebecca Eells, Marilia Barros, Kerry M. Scott, Ioannis Karageorgos, Frank Heinrich, Mathias Losche
Article
Biochemical Research Methods
Rana Ashkar, Hassina Z. Bilheux, Heliosa Bordallo, Robert Briber, David J. E. Callaway, Xiaolin Cheng, Xiang-Qiang Chu, Joseph E. Curtis, Mark Dadmun, Paul Fenimore, David Fushman, Frank Gabel, Kushol Gupta, Frederick Herberle, Frank Heinrich, Liang Hong, John Katsaras, Zvi Kelman, Eugenia Kharlampieva, Gerald R. Kneller, Andrey Kovalevsky, Susan Krueger, Paul Langan, Raquel Lieberman, Yun Liu, Mathias Losche, Edward Lyman, Yimin Mao, John Marino, Carla Mattos, Flora Meilleur, Peter Moody, Jonathan D. Nickels, William B. O'Dell, Hugh O'Neill, Ursula Perez-Salas, Judith Peters, Loukas Petridis, Alexei P. Sokolov, Christopher Stanley, Norman Wagner, Michael Weinrich, Kevin Weiss, Troy Wymore, Yang Zhang, Jeremy C. Smith
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
(2018)
Article
Chemistry, Multidisciplinary
Bradley W. Treece, Paul A. Kienzle, David P. Hoogerheide, Charles F. Majkrzak, Mathias Losche, Frank Heinrich
JOURNAL OF APPLIED CRYSTALLOGRAPHY
(2019)
Article
Biochemistry & Molecular Biology
Matthew P. Pond, Rebecca Eells, Bradley W. Treece, Frank Heinrich, Mathias Losche, Benoit Roux
JOURNAL OF MOLECULAR BIOLOGY
(2020)
Article
Chemistry, Physical
Bradley W. Treece, Frank Heinrich, Arvind Ramanathan, Mathias Losche
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2020)
Article
Chemistry, Multidisciplinary
Frank Heinrich, Paul A. Kienzle, David P. Hoogerheide, Mathias Losche
JOURNAL OF APPLIED CRYSTALLOGRAPHY
(2020)
Article
Multidisciplinary Sciences
Que N. Van, Cesar A. Lopez, Marco Tonelli, Troy Taylor, Ben Niu, Christopher B. Stanley, Debsindhu Bhowmik, Timothy H. Tran, Peter H. Frank, Simon Messing, Patrick Alexander, Daniel Scott, Xiaoying Ye, Matt Drew, Oleg Chertov, Mathias Losche, Arvind Ramanathan, Michael L. Gross, Nicolas W. Hengartner, William M. Westler, John L. Markley, Dhirendra K. Simanshu, Dwight Nissley, William K. Gillette, Dominic Esposito, Frank McCormick, S. Gnanakaran, Frank Heinrich, Andrew G. Stephen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Olivier Soubias, Shashank Pant, Frank Heinrich, Yue Zhang, Neeladri Sekhar Roy, Jess Li, Xiaoying Jian, Marielle E. Yohe, Paul A. Randazzo, Mathias Losche, Emad Tajkhorshid, R. Andrew Byrd