期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 174, 期 1, 页码 164-172出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2010.12.007
关键词
Luffin P1; Ribosome-inactivating peptide; Nuclear magnetic resonance; Anti-HIV-1
资金
- Research Grants Council [HKU 7533/06M, 7755/08M, CUHK 4606/06M]
- Chinese University of Hong Kong
- University Grants Council of Hong Kong SAR [SEG CUHKO8, CUHKO9]
- National Basic Research Program of China [2009CB5223006]
- Eleventh Five-Year Key Scientific and Technological Program of China [2009ZX09501-029, 2008ZX10001-002, 2008ZX10001-015]
- Chinese Academy of Sciences [KSCX2-YW-R-185]
Luffin P1, the smallest ribosome-inactivating peptide from the seeds of Luffa cylindrica was found to have anti-HIV-1 activity in HIV-1 infected C8166 T-cell lines and be able to bind with HIV Rev Response Element. Nuclear magnetic resonance spectroscopy revealed that the Luffin P1 comprises a helix-loop-helix motif, with the two alpha helices tightly associated by two disulfide bonds. Based on our findings, we conclude that unlike the well-studied ribosome-inactivating proteins, which exert their action through N-glycosidase activities, Luffin P1 demonstrates a novel inactivation mechanism probably through the charge complementation with viral or cellular proteins. Our work also provides a new scaffold for the design of novel inhibitors from a simple helical motif. (C) 2010 Elsevier Inc. All rights reserved.
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