期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 171, 期 3, 页码 382-388出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2010.06.001
关键词
Structured illumination microscopy; Liver sinusoidal endothelial cell; Fenestrations; Nitric oxide synthase; Actin
资金
- American Heart Association
- National Science Foundation
- National Center for Research Resources (NCRR) [UL1 RR024146]
- National Institutes of Health [DK66423]
- National Institutes of Health and National Institute of Aging [AG026582]
- Australian National Health and Medical Research Council
- Ageing and Alzheimers Research Foundation
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK066423] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R21AG026582] Funding Source: NIH RePORTER
Fenestrations are pores in liver sinusoidal endothelial cells that filter substrates and debris between the blood and hepatocytes. Fenestrations have significant roles in aging and the regulation of lipoproteins. However their small size (<200 nm) has prohibited any functional analysis by light microscopy. We employed structured illumination light microscopy to observe fenestrations in isolated rat liver sinusoidal endothelial cells with great clarity and spatial resolution. With this method, the three-dimensional structure of fenestrations (diameter 123 +/- 24 nm) and sieve plates was elucidated and it was shown that fenestrations occur in areas of abrupt cytoplasmic thinning (165 +/- 54 nm vs. 292 +/- 103 nm in non-fenestrated regions, P < 0.0001). Sieve plates were not preferentially co-localized with fluorescently labeled F-actin stress fibers and endothelial nitric oxide synthase but appeared to occur in primarily attenuated non-raft regions of the cell membrane. Labyrinthine structures were not seen and all fenestrations were short cylindrical pores. In conclusion, three-dimensional structured illumination microscopy has enabled the unlimited power of fluorescent immunostaining and co-localization to reveal new structural and functional information about fenestrations and sieve plates. (C) 2010 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据