Review
Cell Biology
Nishit Srivastava, Guilherme Pedreira de Freitas Nader, Alice Williart, Romain Rollin, Damien Cuvelier, Alexis Lomakin, Matthieu Piel
Summary: The cell nucleus can adopt a variety of shapes in tissues due to cell deformities caused by cell crowding or migration through dense matrices. Recent studies have shown that the nuclear envelope can form blebs, leading to nuclear envelope opening and uncontrolled nucleocytoplasmic mixing. While blebs are a major source of nuclear instability, they are poorly understood and require more in-depth research.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Materials Science, Ceramics
Lei Yuan, Endong Jin, Chaoyue Li, Zhenli Liu, Chen Tian, Beiyue Ma, Jingkun Yu
Summary: High-purity calcium hexaluminate (CA(6)) porous ceramics were prepared using a novel pectin based gelcasting freeze-drying method, with investigations on the effects of solid content on material properties. The pores in CA(6) ceramics were mainly formed through water molecules sublimation and organic decomposition during freeze-drying. The ceramics exhibited high apparent porosity, low bulk density, low thermal conductivity, and relatively high compressive strength, meeting requirements for functional and structural applications.
CERAMICS INTERNATIONAL
(2021)
Review
Biotechnology & Applied Microbiology
Fabrizio A. Pennacchio, Paulina Nastaly, Alessandro Poli, Paolo Maiuri
Summary: Cells sense and react to various mechanochemical stimuli, reshaping their morphology and adapting their structural organization. Mechanotransduction plays a crucial role in cellular functions, and studies have developed tools to control cell mechanical state. The nucleus serves as an important mechanosensor organelle, governing cell mechanoresponse, and perturbations in nucleus-cytoskeleton connections can influence health and disease.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Yongkai Yuan, Ming Yin, Ling Chen, Fei Liu, Maoshen Chen, Fang Zhong
Summary: This study investigates the impact of calcium ions on the survival rate of probiotics encapsulated in alginate hydrogel. The results show that the freeze-drying survival rate of Lactobacillus rhamnosus GG (LGG) encapsulated in calcium alginate is lower compared to sodium alginate. The loss of survival mainly occurs during the drying process and is positively correlated with the increase of calcium ions concentration. Calcium ions cause damage to the cell wall/membrane integrity, suppress metabolic activity, and reduce intracellular calcium ion concentration, leading to the loss of probiotic viability.
FOOD HYDROCOLLOIDS
(2022)
Article
Engineering, Chemical
Sachchidanand Pandey, Yashaswini Premjit, Anjali Khuntia, Amaresh Kadival, Jayeeta Mitra
Summary: The study aimed to encapsulate Lactobacillus rhamnosus in calcium alginate-maltodextrin hydrogel systems using electrospraying and freeze-drying techniques. The addition of maltodextrin significantly increased encapsulation efficiency and decreased cell viability.
Article
Engineering, Chemical
Mokhtar Dabbour, Rokkaya Sami, Benjamin K. Mintah, Ronghai He, Hafida Wahia, Ebtihal Khojah, Anka Trajkovska Petkoska, Mohammad Fikry
Summary: The influence of freeze and convection drying on the physical, functional, and rheological attributes of sunflower protein and its hydrolysate was studied. Freeze-dried samples had higher lightness, turbidity, bulk density, and particle size values, while having lower browning index. They also showed good solubility and foaming characteristics. Convectively-dried powders exhibited greater viscosity and consistency coefficient, and lower flow behavior index of dispersions.
Review
Biochemistry & Molecular Biology
Ghassan Bkaily, Danielle Jacques
Summary: Calcium is a positively charged ionic species that regulates various cell functions and acts as a second messenger. Abnormal calcium homeostasis can lead to diseases such as cardiovascular disorders, skeletal disorders, immune disorders, secretion disorders, and cancer. Therefore, pharmacological control of calcium transport is important in treating these conditions, particularly in the cardiovascular system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Anna Selezneva, Alasdair J. Gibb, Dean Willis
Summary: The traditional view of the nuclear envelope as an inert physical barrier has been challenged by recent research, which suggests that it plays important roles in regulating cellular functions, particularly in immune cells. The nuclear envelope may serve as a sensor/transducer of mechanical signals and an integrator of mechanical and chemical signals, allowing precise regulation of gene transcription and functionality in immune cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Jennifer M. Roehrl, Rouven Arnold, Karima Djabali
Summary: In Hutchinson-Gilford progeria syndrome (HGPS), NPC clustering mainly occurs in dysmorphic nuclei, with a higher frequency in early passages compared to control cells. Progerin does not disrupt the post-mitotic reassembly of NPCs, but leads to NPC clustering in nuclei with high progerin content. Additionally, nuclear envelope defects during replicative senescence result in NPC clustering in senescent cells with dysmorphic nuclei.
Article
Pharmacology & Pharmacy
Tugrul Mert Serim, Jan Kozak, Annika Rautenberg, Ayse Nurten Ozdemir, Yann Pellequer, Alf Lamprecht
Summary: A new approach using SFD lyospheres(R) for the preparation of fast dissolving porous particles for nasal administration of insulin was proposed in this study, showing promising potential as an effective delivery system. The particles demonstrated low density and high porosity, leading to excellent performance in nasal administration, indicating a highly prospective platform for nasal peptide delivery.
Article
Plant Sciences
Joseph F. McKenna, Hardeep K. Gumber, Zachary M. Turpin, Alexis M. Jalovec, Andre C. Kartick, Katja Graumann, Hank W. Bass
Summary: The study demonstrates that nucleoskeletal proteins NCH1, NCH2, and MKAKU41 in maize have characteristic properties of LINC-associated plant nucleoskeletal proteins, impacting the nuclear periphery structure and overall nuclear architecture.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Haiyue Liu, Jie-Shun Lin, Zhenpeng Luo, Jongho Sun, Xiaowei Huang, Yang Yang, Ji Xu, Yong-Fei Wang, Peng Zhang, Giles E. D. Oldroyd, Fang Xie
Summary: Nuclear Ca2+ oscillations play a crucial role in plant recognition of beneficial microsymbionts, and DMI1 and CNGC15 coordinate the oscillations. Gain-of-function mutations in DMI1 activate the channel complex, leading to spontaneous nuclear Ca2+ oscillations and nodulation. These mutations destabilize the hydrogen bond or salt-bridge network between RCK domains, resulting in structural changes and activation of the channel complex.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Salla Mattola, Vesa Aho, Luisa F. Bustamante-Jaramillo, Edoardo Pizzioli, Michael Kann, Maija Vihinen-Ranta
Summary: Parvoviruses are small non-enveloped single-stranded DNA viruses that rely on the host cell's nuclear transcriptional and replication machinery. They enter the nucleus through nuclear pore complexes or an alternative pathway and undergo replication and infection. Nuclear egress of capsids occurs during nuclear envelope degradation or possibly through other unknown pathways.
MOLECULAR MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Asier Echarri
Summary: Cells have mechanisms to sense and adapt to mechanical forces, with the nucleus being an essential part of this process. It is physically connected to the cytoskeleton and has additional structures within and outside the nucleus that contribute to its mechanosensitivity. This network of mechanosensitive structures ensures that the nucleus can respond to mechanical cues.
Article
Multidisciplinary Sciences
Emily A. Schmitz, Hirohide Takahashi, Erkan Karakas
Summary: This study presents cryo-EM structures of human type-3 IP3 receptors, revealing the molecular mechanism of receptor activation. The structures demonstrate how IP3-induced conformational changes prime the receptor for activation by Ca2+, and how ATP modulates the activity.
NATURE COMMUNICATIONS
(2022)
Article
Dermatology
C. Lecaros, J. Dunstan, F. Villena, D. M. Ashcroft, R. Parisi, C. E. M. Griffiths, S. Hartel, J. T. Maul, C. De la Cruz
Summary: This study estimated the incidence rates of psoriasis in Chile during 2016 and 2017, with national incidence rates of 22.1 and 22.7 per 100,000 person-years, respectively. Late-onset psoriasis was found to be the most common type, and there was a high variation in incidence rates across different regions of the country.
CLINICAL AND EXPERIMENTAL DERMATOLOGY
(2021)
Editorial Material
Biochemical Research Methods
Jason R. Swedlow, Pasi Kankaanpaa, Ugis Sarkans, Wojtek Goscinski, Graham Galloway, Leonel Malacrida, Ryan P. Sullivan, Steffen Hartel, Claire M. Brown, Christopher Wood, Antje Keppler, Federica Paina, Ben Loos, Sara Zullino, Dario Livio Longo, Silvio Aime, Shuichi Onami
Summary: Imaging technologies play a crucial role in understanding biological mechanisms and in diagnosis and therapy in animal and human medicine. Establishing globally applicable guidelines for open image data tools and resources can help advance the rapidly developing fields of biological and biomedical imaging.
Article
Immunology
Amy Monaco, Nicole Canales-Huerta, Jorge Jara-Wilde, Steffen Hartel, Jose Alejandro Chabalgoity, Maria Moreno, Paola Scavone
Summary: Salmonella, a pathogen responsible for a wide range of diseases, triggers host inflammatory responses and recruits innate immune cells. Research has shown that macrophages can release extracellular DNA traps, known as Macrophage Extracellular Traps (METs), which play a crucial role in immobilizing and reducing Salmonella survival, offering a novel immune-mediated defense mechanism against Salmonella infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Cell Biology
Omar A. Ramirez, Alex Cordova, Mauricio Cerda, Pedro Lobos, Steffen Hartel, Andres Couve, Cecilia Hidalgo
Summary: Neuronal Ca2+ signals are essential for synaptic transmission and gene expression changes. Voltage-gated Ca2+ channels and N-methyl-D-aspartate receptors are important for Ca2+ entry during action potential firing. The endoplasmic reticulum (ER), including RyR and IP3R channels, contributes to Ca2+ signal generation in response to neuronal activity. The study investigates the role of RyR-mediated Ca2+ release in IP3-generated Ca2+ signals in dendrites and the potential impact of atlastin on Ca2+ signaling and ER-resident Ca2+ channel distribution.
Article
Multidisciplinary Sciences
Pedro Lobos, Alex Cordova, Ignacio Vega-Vasquez, Omar A. Ramirez, Tatiana Adasme, Jorge Toledo, Mauricio Cerda, Steffen Hartel, Andrea Paula-Lima, Cecilia Hidalgo
Summary: In the hippocampus, the expression of genes involved in learning and memory processes is influenced by Ca2+ signals, which are propagated to the nucleus through Ca2+ release mediated by the ryanodine receptor. This mechanism contributes to CREB phosphorylation, Npas4, and RyR2 expression, playing a crucial role in hippocampal synaptic plasticity and memory processes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biology
Eduardo Pulgar, Cornelia Schwayer, Nestor Guerrero, Loreto Lopez, Susana Marquez, Steffen Hartel, Rodrigo Soto, Carl-Philipp Heisenberg, Miguel L. Concha
Summary: The study uncovers a mechanism where progenitor cells coordinate their movement with adjacent tissues by incomplete delamination during development. This process ensures the collective fate and allocation of cells at the site of differentiation.
Article
Medicine, General & Internal
Camilo G. Sotomayor, Marcelo Mendoza, Victor Castaneda, Humberto Farias, Gabriel Molina, Gonzalo Pereira, Steffen Hartel, Mauricio Solar, Mauricio Araya
Summary: The project aims to address the challenges in medical imaging consumption by developing a content-based image retrieval system and an intelligent interactive visual browser that can display a set of imaging examinations with similar visual content, allowing for efficient search and navigation through large-scale medical imaging repositories. Professionals in the healthcare sector have emphasized the usefulness of this system and further studies are needed for a comprehensive assessment of its performance by medical imaging specialists.
Article
Cell Biology
Maria de los Angeles Juricic Urzua, Javiera Gallardo Rojas, Andres Couve Correa, Mauricio Cerda, Steffen Hartel Grundler, Carolina Gonzalez-Silva
Summary: This study characterized the distribution and dynamics of neuronal ERGIC structures. Long-distance ERGIC transport in dendrites occurs via an intermittent mechanism, with both stationary and mobile structures. Interestingly, this movement depends not only on the integrity of the microtubule cytoskeleton, but also on the actin cytoskeleton.
Article
Immunology
Romina Ulloa, Oreste Corrales, Fernanda Cabrera-Reyes, Jorge Jara-Wilde, Juan Jose Saez, Christopher Rivas, Jonathan Lagos, Steffen Haertel, Clara Quiroga, Maria-Isabel Yuseff, Jheimmy Diaz-Munoz
Summary: This study reveals that changes in nuclear morphology and positioning are critical for the formation of immune synapse and efficient antigen extraction in B cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Clinical Neurology
Julian Henao-Restrepo, Carolina Lopez-Murillo, Pablo Valderrama-Carmona, Natalia Orozco-Santa, Johana Gomez, Johanna Gutierrez-Vargas, Renato Moraga, Jorge Toledo, Jessica Lisa Littau, Steffen Haertel, Joseph F. Arboleda-Velasquez, Diego Sepulveda-Falla, Francisco Lopera, Gloria Patricia Cardona-Gomez, Andres Villegas, Rafael Posada-Duque
Summary: This study provides insights into the role of astrocytes in the development and protection of Alzheimer's disease. The study reveals differences in astrocyte reactions in different forms of the disease and suggests the potential relevance of the gliovasculature.
Article
Cell Biology
Karina Palma, Iskra A. Signore, Margarita M. Meynard, Jazmin Ibarra, Lorena Armijo-Weingart, Marcos Cayuleo, Steffen Hartel, Miguel L. Concha
Summary: This study demonstrates that the parapineal organ, while morphologically lost during ontogeny in zebrafish, its cells are integrated into the dorsal habenular nucleus and retain their structural, neurochemical, and connective features. The findings suggest that parapineal cells form an integral part of a neural circuit associated with the left habenula and may play a role as local modulators. This study provides insights into the evolutionary developmental mechanism underlying the presence/absence of the parapineal organ in teleosts and vertebrates.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Zhongming Ma, Usha Paudel, J. Kevin Foskett
Summary: The intensity of taste is strongly influenced by temperature, but there is a lack of comprehensive understanding about the physiological, hedonic, and commercial implications of this relationship. This study used patch-clamp electrophysiology to investigate the effects of temperature on taste-bud cell electrical excitability, revealing that temperature significantly affects the electrical activity of type II taste-bud cells. These findings provide a mechanism for understanding how temperature influences taste sensitivity and perception.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Microbiology
Paola Scavone, Victoria Iribarnegaray, Maria Jose Gonzalez, Nicolas Navarro, Nicole Caneles-Huerta, Jorge Jara-Wilde, Steffen Hartel, Pablo Zunino
Summary: Flagella of Proteus mirabilis play a role in biofilm formation, although their lack does not completely prevent biofilm generation. Impairment of flagellar function can contribute to biofilm prevention.
REVISTA ARGENTINA DE MICROBIOLOGIA
(2023)
Article
Oncology
Liz Maria de Almeida, Sandra Cortes, Marta Vilensky, Olivia Valenzuela, Laura Cortes-Sanabria, Mirian de Souza, Rafael Alonso Barbeito, Eliana Abdelhay, Nora Artagaveytia, Adrian Daneri-Navarro, Andrea S. Llera, Bettina Muller, Osvaldo L. Podhajcer, Carlos Velazquez, Elsa Alcoba, Isabel Alonso, Alicia Bravo, Natalia Camejo, Dirce Maria Carraro, Monica Castro, Sandra Cataldi, Alfonso Cayota, Mauricio Cerda, Alicia Colombo, Susanne Crocamo, Alicia Del Toro-Arreola, Raul Delgadillo-Cristerna, Lucia Delgado, Marisa Dreyer Breitenbach, Elmer Fernandez, Jorge Fernandez, Wanda Fernandez, Ramon A. Franco-Topete, Fancy Gaete, Jorge Gomez, Leivy P. Gonzalez-Ramirez, Marisol Guerrero, Susan A. Gutierrez-Rubio, Beatriz Jalfin, Alejandra Lopez-Vazquez, Dora Loria, Silvia Miguez, Andres de J. Moran-Mendoza, Gilberto Morgan-Villela, Carina Mussetti, Maria Aparecida Nagai, Antonio Oceguera-Villanueva, Rui M. Reis, Javier Retamales, Robinson Rodriguez, Cristina Rosales, Efrain Salas-Gonzalez, Laura Segovia, Juan M. Sendoya, Aida A. Silva-Garcia, Stella Vina, Livia Zagame, Beth Jones, Moyses Szklo
Summary: The molecular profile of breast cancer in Latin-American women was studied in five countries. The study described the socioeconomic characteristics, risk factors, prognostic factors, and molecular subtypes, and estimated the 60-month overall cumulative survival probabilities. Factors such as country of residence, intrinsic subtype, age, and tumor stage were found to be associated with overall survival.
FRONTIERS IN ONCOLOGY
(2022)
Proceedings Paper
Computer Science, Artificial Intelligence
V Castaneda, C. A. Figueroa, F. Horta, S. Vargas, A. Garcia, J. Jara-Wilde, S. Hartel
Summary: Over the past decade, there has been substantial growth in the use of Sperm Chromatin Dispersion tests (SCDt) to assess DNA fragmentation in human sperm, with SCDt showing clear advantages over other techniques. However, visual evaluation in SCDt remains subjective. The goal of this study was to develop an automated and objective image analysis method for evaluating DNA fragmentation in human sperm using SCDt images.
INTELLIGENT COMPUTING SYSTEMS (ISICS 2022)
(2022)
