期刊
PLANT SCIENCE
卷 238, 期 -, 页码 64-72出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2015.06.001
关键词
Arabidopsis; Anthocyanin; PIFs; Red light
资金
- National Natural Science Foundation of China [31170225, 31201145, 31360296]
- China Postdoctoral Science Foundation [2011M501489]
- Fundamental Research Funds for the Central Universities [lzujbky-2011-36, lzujbky-2015-91]
Light is an important environmental factor inducing anthocyanin accumulation in plants. Phytochrome-interacting factors (PIFs) have been shown to be a family of bHLH transcription factors involved in light signaling in Arabidopsis. Red light effectively increased anthocyanin accumulation in wild-type Col-0, whereas the effects were enhanced in pif4 and pif5 mutants but impaired in overexpression lines PIF4OX and PIF5OX, indicating that PIF4 and PIF5 are both negative regulators for red light-induced anthocyanin accumulation. Consistently, transcript levels of several genes involved in anthocyanin biosynthesis and regulatory pathway, including CHS,F3'H,DFR,LDOX,PAP1 and 778, were significantly enhanced in mutants pif4 and pif5 but decreased in PIF4OX and PIE5OX compared to in Col-0, indicating that PIF4 and PIF5 are transcriptional repressor of these gene. Transient expression assays revealed that PIF4 and PIF5 could repress red light-induced promoter activities of F3'H and DFR in Arabidopsis protoplasts. Furthermore, chromatin immunoprecipitation-quantitative PCR (ChIP-OCR) test and electrophoretic mobility shift assay (EMSA) showed that PIF5 could directly bind to G-box motifs present in the promoter of DFR. Taken together, these results suggest that PIF4 and PIF5 negatively regulate red light-induced anthocyanin accumulation through transcriptional repression of the anthocyanin biosynthetic genes in Arabidopsis. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据