Article
Optics
Ying Li, Xin Liu, Zhongming Yang, Zhaojun Liu
Summary: This paper proposes a high-precision phase retrieval method based on deep learning to extract the Zernike coefficients from a single-frame interferogram with an irregular-shaped aperture. Simulations and experiments demonstrate that the proposed method has high measurement accuracy and robustness, making it useful for measuring optical surfaces with irregular apertures.
Article
Optics
Ying Li, Xin Liu, Zhongming Yang, Zhaojun Liu
Summary: This paper proposes a high-precision phase retrieval method based on deep learning to extract the Zernike coefficients from a single-frame interferogram with an irregular-shaped aperture. The results show that the proposed method has high measurement accuracy and robustness, comparable to commercial phase-shifting interferometers, and is useful for measuring optical surfaces with irregular apertures.
Article
Biochemical Research Methods
Ivan Lazic, Maarten Wirix, Max Leo Leidl, Felix de Haas, Daniel Mann, Maximilian Beckers, Evgeniya Pechnikova, Knut Muller-Caspary, Ricardo Egoavil, Eric G. T. Bosch, Carsten Sachse
Summary: This paper explores the use of scanning transmission electron microscopy (STEM) and integrated differential phase contrast mode (iDPC-STEM) for biomolecular structure elucidation of vitrified biological samples. It demonstrates successful cryo-EM structure determination at 6.5 and 3.5 angstrom resolution for keyhole limpet hemocyanin and tobacco mosaic virus, respectively, using STEM imaging.
Article
Computer Science, Information Systems
Shiyu Zhang, Kai Lin, Hongsong Li, Lu Lu
Summary: In this study, a Zernike SPI (ZSPI) technique is proposed to reduce the number of illumination patterns and reconstruction time while maintaining robustness. Numerical simulations show that ZSPI can achieve satisfactory reconstruction results at low sample ratios. Further simulations and experiments demonstrate that ZSPI has higher imaging quality and requires less imaging time, especially for low-frequency targets.
Article
Optics
Oriol Roche I. Morgo, Jure Aleksejev, Alberto Astolfo, Savvas Savvidis, Mattia F. M. Gerli, Silvia Cipiccia, Alessandro Olivo, Charlotte K. Hagen
Summary: Cycloidal computed tomography combines beam modulation with under-sampling to provide high-resolution images in short scan times. A new position tracking technique using a sharp edge has been developed, which improves the reconstruction process and enables the implementation of innovative sampling schemes.
Article
Nanoscience & Nanotechnology
Yinxu Bian, Yannan Jiang, Weijie Deng, Renbing Shen, Hua Shen, Cuifang Kuang
Summary: A novel singlet virtual Zernike phase contrast microscopy setup is reported in this manuscript, which achieves Zernike phase contrast without the need for a Zernike phase plate through deep learning computational imaging method, with a computational time less than 100 ms. This method is proven to be competitive with research-level conventional Zernike phase contrast microscope and effective for unstained transparent pathological tumor tissue slides.
Article
Multidisciplinary Sciences
Mikolaj Rogalski, Jose Angel Picazo-Bueno, Julianna Winnik, Piotr Zdankowski, Vicente Mico, Maciej Trusiak
Summary: Building on Gabor's principle, digital in-line holographic microscopy allows efficient investigations of large volumes in space and time. This has a significant impact on particle tracking in various fields of science and technology. The DarkTrack algorithm presented in this work offers a versatile, fast, precise, and robust 4D holographic tracking method based on high-contrast dark fields. It fills an important gap in holographic particle tracking technology and enables benchmarking and assessment of tracking algorithms.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Matteo Busi, Jiazhou Shen, Michael Bacak, Marie Christine Zdora, Jan Capek, Jacopo Valsecchi, Markus Strobl
Summary: This study introduces a novel multi-directional neutron dark-field imaging approach that utilizes a single absorption grating to simultaneously probe multiple scattering directions. The method successfully resolves the microstructural properties of materials and overcomes limitations of conventional neutron dark-field imaging in assessing complex heterogeneous anisotropic microstructures.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
D. Herreros, J. Kiska, E. Ramirez-Aportela, J. Filipovic, J. M. Carazo, C. O. S. Sorzano
Summary: The main purpose of CryoEM Single Particle Analysis is to reconstruct the three-dimensional structure of a macromolecule. ZART is a new Algebraic Reconstruction Technique that can include continuous flexibility information to improve resolution and reduce motion blurring.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Teun A. P. M. Huijben, Hamidreza Heydarian, Alexander Auer, Florian Schueder, Ralf Jungmann, Sjoerd Stallinga, Bernd Rieger
Summary: Particle fusion in single molecule localization microscopy can improve signal-to-noise ratio, but is limited by structural heterogeneity. The authors demonstrate an unsupervised classification method that can differentiate structurally different DNA origami structures without prior knowledge.
NATURE COMMUNICATIONS
(2021)
Article
Optics
Valerio Bellucci, Marie-Christine Zdora, Ladislav Mikes, Sarlota Birnsteinova, Peter Oberta, Marco Romagnoni, Andrea Mazzolari, Pablo Villanueva-Perez, Rajmund Mokso, Christian David, Mikako Makita, Silvia Cipiccia, Jozef Ulicny, Alke Meents, Adrian P. Mancuso, Henry N. Chapman, Patrik Vagovic
Summary: The characterisation of fast phenomena at the microscopic scale is necessary for understanding various key technologies and processes, such as the responses of materials to loads and shocks, material processing, additive manufacturing, microfluidics, and fuel mixing in combustion. This paper demonstrates a method to record three-dimensional X-ray movies of irreversible processes with high resolutions and frame rates by combining stereo phase-contrast images. The method can be further extended to include more than two simultaneous views, and when combined with X-ray free-electron lasers (XFELs), it can achieve movies capable of resolving 3D trajectories at high velocities.
Article
Optics
Michelle K. Croughan, Ying Ying How, Allan Pennings, Kaye S. Morgan
Summary: Directional dark-field imaging is a sensitive x-ray modality that captures anisotropic scattering from sub-pixel sample microstructures. A single-grid imaging setup and a retrieval algorithm can extract dark-field parameters, even in the presence of high image noise. This method allows for low-dose and time-sequence imaging.
Article
Materials Science, Multidisciplinary
Hamish G. Brown, Philipp M. Pelz, Shang-Lin Hsu, Zimeng Zhang, Ramamoorthy Ramesh, Katherine Inzani, Evan Sheridan, Sinead M. Griffin, Marcel Schloz, Thomas C. Pekin, Christoph T. Koch, Scott D. Findlay, Leslie J. Allen, Mary C. Scott, Colin Ophus, Jim Ciston
Summary: The increasing interest in three-dimensional nanostructures has led to the development of electron microscopy techniques capable of imaging at or below the nanoscale in three dimensions. In this study, a reconstruction algorithm is presented that uses a focal series of four-dimensional scanning transmission electron microscopy (4D-STEM) data. The algorithm is applied to a lead iridate and yttrium-stabilized zirconia heterostructure, demonstrating the visibility of specific atomic columns in the reconstructed volume. A comparison is made with alternative techniques such as depth sectioning using differential phase contrast scanning transmission electron microscopy (DPC-STEM) and multislice ptychographic reconstruction.
MICROSCOPY AND MICROANALYSIS
(2022)
Article
Materials Science, Multidisciplinary
L. Bondarenko, A. Y. Tupchaya, Y. E. Vekovshinin, D. Gruznev, A. N. Mihalyuk, N. Denisov, A. Matetskiy, A. Zotov, A. A. Saranin
Summary: Atomic sandwiches consisting of In layers with different thicknesses have been grown on Si(111) and their properties have been studied using experimental techniques and theoretical calculations. The results show that the In phases formed on the NiSi2/Si(111) substrate have counterparts on the bare Si(111) surface. The structural, electronic, and transport properties of the In layers have been elucidated in detail.
Article
Physics, Multidisciplinary
Riccardo Scheda, Silvia Vitali, Enrico Giampieri, Gianni Pagnini, Isabella Zironi
Summary: Cellular contacts influence the way cells migrate in a cohesive group compared to a single free cell. A novel pipeline is proposed for analyzing phase contrast images of wound healing scratch assays, with the goal of extracting single particle trajectories describing wound closure dynamics. The method involves using the cell membrane at the wound edge as a unicum for stochastic motion analysis.
