期刊
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 118, 期 1-2, 页码 29-40出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jsbmb.2009.09.015
关键词
TCTP; VDR; Yeast two-hybrid; Protein-protein interaction; Skin
资金
- EC [512020]
- Austrian Science Fund FWF (Vienna, Austria) [S9302-B05]
- PMU (Salzburg, Austria) [06/03/020]
- Austrian Academy of Sciences (Vienna, Austria)
Translationally controlled tumour protein (TCTP) is an evolutionarily highly conserved molecule implicated in many processes related to cell cycle progression, proliferation and growth, to the protection against harmful conditions including apoptosis and to the human allergic response. We are showing here that after application of mild oxidative stress, human TCTP relocates from the cytoplasm to the nuclei of HaCaT keratinocytes where it directly associates with the ligand-binding domain of endogenous vitamin D-3 receptor (VDR) through its helical domain 2 (AA 71-132). Interestingly, the latter harbours a putative nuclear hormone receptor coregulatory LxxLL-like motif which seems to be involved in the interaction. Moreover, we demonstrate that VDR transcriptionally induces the expression of TCTP by binding to a previously unknown VDR response element within the TCTP promotor. Conversely, ectopically over-expressed TCTP downregulates the amount of VDR on both mRNA as well as protein level. These data, to conclude, suggest a kind of feedback regulation between TCTP and VDR to regulate a variety of (Ca2+ dependent) cellular effects and in this way further underscore the physiological relevance of this novel protein-protein interaction. (C) 2009 Elsevier Ltd. All rights reserved.
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