期刊
PLANT MOLECULAR BIOLOGY
卷 89, 期 3, 页码 229-242出版社
SPRINGER
DOI: 10.1007/s11103-015-0357-3
关键词
Dof; OsC4PPDK; Transcription factor; Rice
资金
- CAS-KNAW Joint Ph.D. Training Programme of the Royal Netherlands Academy of Arts and Sciences (KNAW) [05-PhD-02]
- KNAW Programme Scientific Alliance [04-PSA-BD-04]
- KNAW China Exchange Programmes (CEP) [04CD022, 06CDO033, 07CDP005, 08CDP042, 10CDP023]
Relative little is known about the functions of the so-called Dof zinc factors in plants. Here we report on the analysis of OsDof25 and show a function in regulation of the important C4 photosynthesis gene, OsC4PPDK in rice. Over-expression of OsDof25 enhanced the expression of OsC4PPDK in transient expression experiments by binding in a specific way to a conserved Dof binding site which was confirmed by yeast and in vitro binding studies. Expression studies using promoter GUS plants as well as qPCR experiments showed that OsDof25 expressed in different tissues including both photosynthetic and non-photosynthetic organs and that expression of OsDof25 was partially overlapping with the OsC4PPDK gene. Conclusive evidence for a role of OsDof25 in regulation of C4PPDK came from loss-of-function and gain-of-function experiments with transgenic rice, which showed that down-regulation or over-expression of OsDof25 correlated with OsC4PPDK expression and that OsDof25 has functions as transcriptional activator.
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